Cloned (Comment) | Organism |
---|---|
expression in HEK293 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A429G | the ratio of maximal velocity to Km-value is 75% of the value for the wild-type enzyme | Homo sapiens |
E431A | strong decrease in aminopeptidase activity towards Leu-4-nitroanilide | Homo sapiens |
E431D | strong decrease in aminopeptidase activity towards Leu-4-nitroanilide | Homo sapiens |
E465D | strong decrease in aminopeptidase activity towards Leu-4-nitroanilide | Homo sapiens |
E465Q | strong decrease in aminopeptidase activity towards Leu-4-nitroanilide | Homo sapiens |
E487D | strong decrease in aminopeptidase activity towards Leu-4-nitroanilide | Homo sapiens |
E487Q | inactive mutant enzyme | Homo sapiens |
G428A | the ratio of maximal velocity to Km-value is 90% of the value for the wild-type enzyme | Homo sapiens |
H464Y | strong decrease in aminopeptidase activity towards Leu-4-nitroanilide | Homo sapiens |
H468Y | strong decrease in aminopeptidase activity towards Leu-4-nitroanilide | Homo sapiens |
M430E | strong decrease in aminopeptidase activity towards Leu-4-nitroanilide | Homo sapiens |
M430I | the ratio of maximal velocity to Km-value is 6.2% of the value for the wild-type enzyme | Homo sapiens |
M430K | the ratio of maximal velocity to Km-value is 3.3% of the value for the wild-type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.243 | - |
Leu-4-nitroanilide | pH 7.4, 37°C, mutant enzyme A429G | Homo sapiens | |
0.331 | - |
Cys-4-nitroanilide | pH 7.4, 37°C, wild-type enzyme | Homo sapiens | |
0.4567 | - |
Leu-4-nitroanilide | pH 7.4, 37°C, mutant enzyme M430I | Homo sapiens | |
0.488 | - |
Leu-4-nitroanilide | pH 7.4, 37°C, wild-type enzyme | Homo sapiens | |
0.529 | - |
Arg-4-nitroanilide | pH 7.4, 37°C, wild-type enzyme | Homo sapiens | |
0.593 | - |
Leu-4-nitroanilide | pH 7.4, 37°C, mutant enzyme G428A | Homo sapiens | |
0.9148 | - |
Leu-4-nitroanilide | pH 7.4, 37°C, mutant enzyme M430K | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | contains the characteristic Zn2+-coordination sequence element, HEXXH-(18-64X)-E | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Arg-4-nitroanilide + H2O | - |
Homo sapiens | Arg + 4-nitroaniline | - |
? | |
Cys-4-nitroanilide + H2O | - |
Homo sapiens | Cys + 4-nitroaniline | - |
? | |
Leu-4-nitroanilide + H2O | - |
Homo sapiens | Leu + 4-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
insulin-regulated aminopeptidase | - |
Homo sapiens |
IRAP | - |
Homo sapiens |
oxytocinase | - |
Homo sapiens |