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Literature summary for 3.4.11.3 extracted from
- Gene cloning and characterization of PepC, a cysteine aminopeptidase from Streptococcus thermophilus, with sequence similarity to the eucaryotic bleomycin hydrolase (1994), J. Biochem., 224, 497-506.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Streptococcus thermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | - |
Streptococcus thermophilus |  |
| iodoacetic acid | - |
Streptococcus thermophilus | |
| L-trans-epoxysuccinyl-leucinamido(4-guanidino)-butane | E64 | Streptococcus thermophilus | |
| N-alpha-tosyl-L-lysylchloromethane | - |
Streptococcus thermophilus | |
| N-ethylmaleimide | - |
Streptococcus thermophilus | |
| p-hydroxymercuribenzoate | - |
Streptococcus thermophilus | |
| tosylphenylalanine-chloromethane | - |
Streptococcus thermophilus | |
| Zn2+ | - |
Streptococcus thermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3 | - |
L-Lysine-p-nitroanilide | - |
Streptococcus thermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
6 * 50000, SDS-PAGE | Streptococcus thermophilus |
| 300000 | - |
gel filtration | Streptococcus thermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus thermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Streptococcus thermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Streptococcus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-lysine-p-nitroanilide + H2O | - |
Streptococcus thermophilus | L-lysine + p-nitroaniline | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 50000, SDS-PAGE | Streptococcus thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Streptococcus thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Streptococcus thermophilus |
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Release 2023.1 (January 2023)
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