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Literature summary for 3.4.11.3 extracted from

  • Roy, A.C.; Sen, D.K.; Ratnam, S.S.
    Fractionation and characterization of oxytocinases in human semen (1989), J. Reprod. Fertil., 87, 163-168.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Co2+ reverses EDTA inhibition Homo sapiens
Cu2+
-
Homo sapiens
EDTA
-
Homo sapiens
prostaglandin E-1 at pH 6.2, not at pH 6.8 and 7. 4 Homo sapiens
prostaglandin E2 at pH 6.2, not at pH 6.8 and 7. 4 Homo sapiens
prostaglandin F-2alpha at pH 6.2, not at pH 6.8 and 7. 4 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
S-benzyl-L-cysteine-p-nitroanilide pH 6.2, SP I Homo sapiens
0.236
-
S-benzyl-L-cysteine-p-nitroanilide pH 6.2, SP II Homo sapiens
0.405
-
S-benzyl-L-cysteine-p-nitroanilide pH 7.4, SP I Homo sapiens
0.441
-
S-benzyl-L-cysteine-p-nitroanilide pH 7.4, SP II Homo sapiens
0.504
-
L-leucine-p-nitroanilide pH 7.4 Homo sapiens
0.647
-
L-leucine-p-nitroanilide pH 7.4, SP I Homo sapiens
0.672
-
L-leucine-p-nitroanilide pH 6.2, SP II Homo sapiens
0.867
-
L-leucine-p-nitroanilide pH 6.2, SP I Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ reverses EDTA inhibition Homo sapiens
Cu2+ partly reverses EDTA inhibition Homo sapiens
Zn2+ metalloenzyme Homo sapiens
Zn2+ reverses EDTA inhibition Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
300000
-
gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
2 isoenzymes: SP I, SP II
-

Source Tissue

Source Tissue Comment Organism Textmining
semen
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-benzyl-L-cysteine-p-nitroanilide + H2O
-
Homo sapiens L-benzyl-L-cysteine + p-nitroaniline
-
?
S-benzyl-L-cysteine-p-nitroanilide + H2O
-
Homo sapiens S-benzyl-L-cysteine + p-nitroaniline
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
S-benzyl-L-cysteine-p-nitroanilide, SP I Homo sapiens
7
-
S-benzyl-L-cysteine-p-nitroanilide, SP II Homo sapiens
7.2
-
L-leucine-p-nitroanilide, SP I Homo sapiens
7.4
-
L-leucine-p-nitroanilide, SP II Homo sapiens