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Literature summary for 3.4.11.25 extracted from
- Mutation of active site serine residue with cysteine displays change in acyl-acceptor preference of beta-peptidyl aminopeptidase from Pseudomonas aeruginosa PAO1 (2014), Appl. Microbiol. Biotechnol., 98, 1631-1640.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Pseudomonas aeruginosa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S237C | mutant synthesizes beta-homoGly-beta-homoGly-4-nitroanilide from beta-homoGly-4-nitroanilide efficiently without degradation, indicating that because of the mutation, the enzyme came to recognize only the amine group as an acyl acceptor instead of water. When using cysteamine as an acyl acceptor, beta-homoGly-cysteamine is synthesized by the mutant only in the reaction using S237C. Mutant S237C is unable to synthesize beta-homoGly-cystamine when using cystamine as an acyl acceptor, although it is synthesized by wild-type | Pseudomonas aeruginosa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0073 | - |
beta-homoGly 4-nitroanilide | wild-type, pH 9.0, 25°C | Pseudomonas aeruginosa |  |
| 0.56 | - |
beta-homoGly 4-nitroanilide | wild-type, pH 6.0, 25°C | Pseudomonas aeruginosa | |
| 0.72 | - |
beta-homoGly 4-nitroanilide | mutant S237C, pH 9.0, 25°C | Pseudomonas aeruginosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 14000 | - |
x * 25000, and x * 14000, SDS-PAGE | Pseudomonas aeruginosa |
| 25000 | - |
x * 25000, and x * 14000, SDS-PAGE | Pseudomonas aeruginosa |
| 42000 | - |
gel filtration | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | Q9I3M6 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 beta-homoGly 4-nitroanilide | - |
Pseudomonas aeruginosa | beta-homoGly-beta-homoGly 4-nitroanilide + 4-nitroaniline | product of mutant S237C, synthesized efficiently without degradation | ? | |
| beta-homoGly 4-nitroanilide + H2O | - |
Pseudomonas aeruginosa | beta-homoGly + 4-nitroaniline | wild-type enzyme shows beta-homoGly-beta-homoGly-4-nitroanilide synthetic activity, but the product vanishes in a few min and converts to free beta-homoGly | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | x * 25000, and x * 14000, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BapF | - |
Pseudomonas aeruginosa |
| PA1486 | - |
Pseudomonas aeruginosa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
wild-type, stable up to | Pseudomonas aeruginosa |
| 60 | - |
mutant S237C, stable up to | Pseudomonas aeruginosa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1090 | - |
beta-homoGly 4-nitroanilide | wild-type, pH 9.0, 25°C | Pseudomonas aeruginosa | |
| 1410 | - |
beta-homoGly 4-nitroanilide | wild-type, pH 6.0, 25°C | Pseudomonas aeruginosa | |
| 4540 | - |
beta-homoGly 4-nitroanilide | mutant S237C, pH 9.0, 25°C | Pseudomonas aeruginosa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | wild-type | Pseudomonas aeruginosa |
| 8 | 9.5 | mutant S237C | Pseudomonas aeruginosa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | - |
Pseudomonas aeruginosa |
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