Bacterial beta-peptidyl aminopeptidases with unique substrate specificities for beta-oligopeptides and mixed beta,alpha-oligopeptides
Geueke, B.; Heck, T.; Limbach, M.; Nesatyy, V.; Seebach, D.; Kohler, H.P.; FEBS J. 273, 5261-5272 (2006) 
Data extracted from this reference:
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
no inhibition: EDTA (0.1 mM, 1 mM and 10 mM), leupeptin (0.01 mM, 0.1 mM and 1 mM), phenylmethanesulfonyl fluoride (1 mM and 10 mM), bestatin (0.01 mM and 0.1 mM), and 1,10-phenanthroline (1 mM and 10 mM)
Sphingosinicella microcystinivorans
additional information
no inhibition: EDTA (0.1 mM, 1 mM and 10 mM), leupeptin (0.01 mM, 0.1 mM and 1 mM), phenylmethanesulfonyl fluoride (1 mM and 10 mM), bestatin (0.01 mM and 0.1 mM), and 1,10-phenanthroline (1 mM and 10 mM)
Sphingosinicella xenopeptidilytica
Pefabloc SC
0.4 mM and 4 mM, complete inhibition of hydrolysis of beta-homoVal-beta-homoAla-beta-homoLeu
Sphingosinicella microcystinivorans
Pefabloc SC
0.4 mM and 4 mM, complete inhibition of hydrolysis of beta-homoVal-beta-homoAla-beta-homoLeu
Sphingosinicella xenopeptidilytica
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.4
-
beta-Ala-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella microcystinivorans
8.2
-
beta-Ala-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
9
-
beta-homoVal-beta-homoAla-beta-homoLeu
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
20
-
beta-homoAla-beta-homoLeu
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
39
-
beta-homoVal-beta-homoAla-beta-homoLeu
pH 8.0, 25°C
Sphingosinicella microcystinivorans
41
-
beta-homoAla-beta-homoLeu
pH 8.0, 25°C
Sphingosinicella microcystinivorans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
13144
-
alpha4beta4, 4 * 13144 + 4 * 25333, calculated from sequence
Sphingosinicella microcystinivorans
13168
-
alpha4beta4, 4 * 13168 + 4 * 25465, MALDI-TOF MS
Sphingosinicella microcystinivorans
25333
-
alpha4beta4, 4 * 13144 + 4 * 25333, calculated from sequence
Sphingosinicella microcystinivorans
25465
-
alpha4beta4, 4 * 13168 + 4 * 25465, MALDI-TOF MS
Sphingosinicella microcystinivorans
130000
-
gel filtration
Sphingosinicella microcystinivorans
130000
-
gel filtration
Sphingosinicella xenopeptidilytica
150200
-
electrospray ionisation mass spectrometry
Sphingosinicella xenopeptidilytica
155800
-
electrospray ionisation mass spectrometry
Sphingosinicella microcystinivorans
Organism
Organism
UniProt
Commentary
Textmining
Sphingosinicella microcystinivorans
A0MTQ2
-
-
Sphingosinicella microcystinivorans Y2
A0MTQ2
-
-
Sphingosinicella xenopeptidilytica
Q52VH2
-
-
Sphingosinicella xenopeptidilytica 3-2W4
Q52VH2
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
proteolytic modification
cleavage of the Y2 BapA preprotein between the conserved residues N275 and S276
Sphingosinicella microcystinivorans
Purification (Commentary)
Purification (Commentary)
Organism
recombinant
Sphingosinicella microcystinivorans
recombinant
Sphingosinicella xenopeptidilytica
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.2
-
pH 8.0, 25°C, substrate: beta-Ala-p-nitroanilide
Sphingosinicella microcystinivorans
0.62
-
pH 8.0, 25°C, substrate: beta-Ala-p-nitroanilide
Sphingosinicella xenopeptidilytica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
beta-Ala-Ile-beta-homoTyr + H2O
0.3% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica
beta-Ala + Ile-beta-homoTyr
-
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
1.5% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans
beta-Ala + Ile-beta-homoTyr
-
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
0.3% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica 3-2W4
beta-Ala + Ile-beta-homoTyr
-
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
1.5% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans Y2
beta-Ala + Ile-beta-homoTyr
-
-
-
?
beta-Ala-L-His + H2O
i.e. carnosine, 0.8% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica
beta-Ala + L-His
-
-
-
?
beta-Ala-L-His + H2O
i.e. carnosine, 2% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans
beta-Ala + L-His
-
-
-
?
beta-Ala-L-His + H2O
i.e. carnosine, 0.8% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica 3-2W4
beta-Ala + L-His
-
-
-
?
beta-Ala-L-His + H2O
i.e. carnosine, 2% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans Y2
beta-Ala + L-His
-
-
-
?
beta-Ala-p-nitroanilide + H2O
-
708467
Sphingosinicella xenopeptidilytica
beta-Ala + p-nitroaniline
-
-
-
?
beta-Ala-p-nitroanilide + H2O
-
708467
Sphingosinicella microcystinivorans
beta-Ala + p-nitroaniline
-
-
-
?
beta-Ala-p-nitroanilide + H2O
-
708467
Sphingosinicella xenopeptidilytica 3-2W4
beta-Ala + p-nitroaniline
-
-
-
?
beta-Ala-p-nitroanilide + H2O
-
708467
Sphingosinicella microcystinivorans Y2
beta-Ala + p-nitroaniline
-
-
-
?
beta-homoAla-beta-homoLeu + H2O
preferred substrate
708467
Sphingosinicella microcystinivorans
beta-homoAla-beta-homoLeu
-
-
-
?
beta-homoAla-beta-homoLeu + H2O
35% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica
beta-homoAla-beta-homoLeu
-
-
-
?
beta-homoAla-beta-homoLeu + H2O
35% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica 3-2W4
beta-homoAla-beta-homoLeu
-
-
-
?
beta-homoAla-beta-homoLeu + H2O
preferred substrate
708467
Sphingosinicella microcystinivorans Y2
beta-homoAla-beta-homoLeu
-
-
-
?
beta-homoArg-Ile-beta-homoTyr + H2O
0.2% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica
beta-homoArg + Ile-beta-homoTyr
-
-
-
?
beta-homoArg-Ile-beta-homoTyr + H2O
3.4% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans
beta-homoArg + Ile-beta-homoTyr
-
-
-
?
beta-homoGln-Ile-beta-homoTyr + H2O
0.2% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica
beta-homoGln + Ile-beta-homoTyr
-
-
-
?
beta-homoGln-Ile-beta-homoTyr + H2O
0.3% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans
beta-homoGln + Ile-beta-homoTyr
-
-
-
?
beta-homoHis-Ile-beta-homoTyr + H2O
0.3% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica
beta-homoHis + Ile-beta-homoTyr
-
-
-
?
beta-homoHis-Ile-beta-homoTyr + H2O
0.4% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans
beta-homoHis + Ile-beta-homoTyr
-
-
-
?
beta-homoLeu-Ile-beta-homoTyr + H2O
12% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans
beta-homoLeu + Ile-beta-homoTyr
-
-
-
?
beta-homoLeu-Ile-beta-homoTyr + H2O
61% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica
beta-homoLeu + Ile-beta-homoTyr
-
-
-
?
beta-homoLys-Ile-beta-homoTyr + H2O
0.5% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans
beta-homoLys + Ile-beta-homoTyr
-
-
-
?
beta-homoLys-Ile-beta-homoTyr + H2O
0.5% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica
beta-homoLys + Ile-beta-homoTyr
-
-
-
?
beta-homoPhe-Ile-beta-homoTyr + H2O
15% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans
beta-homoPhe + Ile-beta-homoTyr
-
-
-
?
beta-homoPhe-Ile-beta-homoTyr + H2O
22% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica
beta-homoPhe + Ile-beta-homoTyr
-
-
-
?
beta-homoSer-Ile-beta-homoTyr + H2O
13% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans
beta-homoSer + Ile-beta-homoTyr
-
-
-
?
beta-homoSer-Ile-beta-homoTyr + H2O
3% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica
beta-homoSer + Ile-beta-homoTyr
-
-
-
?
beta-homoThr-Ile-beta-homoTyr + H2O
1.6% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans
beta-homoThr + Ile-beta-homoTyr
-
-
-
?
beta-homoThr-Ile-beta-homoTyr + H2O
2% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica
beta-homoThr + Ile-beta-homoTyr
-
-
-
?
beta-homoTrp-Ile-beta-homoTyr + H2O
1.3% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans
beta-homoTrp + Ile-beta-homoTyr
-
-
-
?
beta-homoTrp-Ile-beta-homoTyr + H2O
1.5% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica
beta-homoTrp + Ile-beta-homoTyr
-
-
-
?
beta-homoTyr-Ile-beta-homoTyr + H2O
15% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica
beta-homoTyr + Ile-beta-homoTyr
-
-
-
?
beta-homoTyr-Ile-beta-homoTyr + H2O
7% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans
beta-homoTyr + Ile-beta-homoTyr
-
-
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
preferred substrate
708467
Sphingosinicella xenopeptidilytica
beta-homoVal + beta-homoAla-beta-homoLeu
-
-
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
27% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans
beta-homoVal + beta-homoAla-beta-homoLeu
-
-
-
?
beta-homoVal-Ile-beta-homoTyr + H2O
15% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans
beta-homoVal + Ile-beta-homoTyr
-
-
-
?
beta-homoVal-Ile-beta-homoTyr + H2O
32% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica
beta-homoVal + Ile-beta-homoTyr
-
-
-
?
D-beta-homoVal-Ile-beta-homoTyr + H2O
the rate of cleavage is slower by factor 28 as compared to the L-enantiomer. 0.5% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans
D-beta-homoVal + Ile-beta-homoTyr
-
-
-
?
D-beta-homoVal-Ile-beta-homoTyr + H2O
the rate of cleavage is slower by factor 35 as compared to the L-enantiomer. 0.9% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica
D-beta-homoVal + Ile-beta-homoTyr
-
-
-
?
additional information
the alpha-tripeptide Val-Ala-Leu and bestatin are not accepted as substrates. No degradation of DL-pyroglutamic acid-p-nitroanilide
708467
Sphingosinicella microcystinivorans
?
-
-
-
-
additional information
the alpha-tripeptide Val-Ala-Leu and bestatin are not accepted as substrates. No degradation of DL-pyroglutamyl-p-nitroanilide
708467
Sphingosinicella xenopeptidilytica
?
-
-
-
-
additional information
the alpha-tripeptide Val-Ala-Leu and bestatin are not accepted as substrates. No degradation of DL-pyroglutamyl-p-nitroanilide
708467
Sphingosinicella xenopeptidilytica 3-2W4
?
-
-
-
-
additional information
the alpha-tripeptide Val-Ala-Leu and bestatin are not accepted as substrates. No degradation of DL-pyroglutamic acid-p-nitroanilide
708467
Sphingosinicella microcystinivorans Y2
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
octamer
alpha4beta4, 4 * 13144 + 4 * 25333, calculated from sequence; alpha4beta4, 4 * 13168 + 4 * 25465, MALDI-TOF MS
Sphingosinicella microcystinivorans
Synonyms
Synonyms
Commentary
Organism
3-2W4 BapA
-
Sphingosinicella xenopeptidilytica
Y2 BapA
-
Sphingosinicella microcystinivorans
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Sphingosinicella microcystinivorans
25
-
assay at
Sphingosinicella xenopeptidilytica
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
half-life: 1 h
Sphingosinicella microcystinivorans
60
-
24 h, stable
Sphingosinicella xenopeptidilytica
70
-
5 min, complete inactivation
Sphingosinicella microcystinivorans
70
-
half-life: 26 min
Sphingosinicella xenopeptidilytica
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.13
-
beta-Ala-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella microcystinivorans
0.75
-
beta-Ala-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
4.3
-
beta-homoVal-beta-homoAla-beta-homoLeu
pH 8.0, 25°C
Sphingosinicella microcystinivorans
6
-
beta-homoVal-beta-homoAla-beta-homoLeu
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
12
-
beta-homoAla-beta-homoLeu
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
60
-
beta-homoAla-beta-homoLeu
pH 8.0, 25°C
Sphingosinicella microcystinivorans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9
-
Sphingosinicella xenopeptidilytica
8
-
assay at
Sphingosinicella microcystinivorans
8
-
assay at
Sphingosinicella xenopeptidilytica
10
-
-
Sphingosinicella microcystinivorans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
no inhibition: EDTA (0.1 mM, 1 mM and 10 mM), leupeptin (0.01 mM, 0.1 mM and 1 mM), phenylmethanesulfonyl fluoride (1 mM and 10 mM), bestatin (0.01 mM and 0.1 mM), and 1,10-phenanthroline (1 mM and 10 mM)
Sphingosinicella microcystinivorans
additional information
no inhibition: EDTA (0.1 mM, 1 mM and 10 mM), leupeptin (0.01 mM, 0.1 mM and 1 mM), phenylmethanesulfonyl fluoride (1 mM and 10 mM), bestatin (0.01 mM and 0.1 mM), and 1,10-phenanthroline (1 mM and 10 mM)
Sphingosinicella xenopeptidilytica
Pefabloc SC
0.4 mM and 4 mM, complete inhibition of hydrolysis of beta-homoVal-beta-homoAla-beta-homoLeu
Sphingosinicella microcystinivorans
Pefabloc SC
0.4 mM and 4 mM, complete inhibition of hydrolysis of beta-homoVal-beta-homoAla-beta-homoLeu
Sphingosinicella xenopeptidilytica
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.4
-
beta-Ala-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella microcystinivorans
8.2
-
beta-Ala-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
9
-
beta-homoVal-beta-homoAla-beta-homoLeu
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
20
-
beta-homoAla-beta-homoLeu
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
39
-
beta-homoVal-beta-homoAla-beta-homoLeu
pH 8.0, 25°C
Sphingosinicella microcystinivorans
41
-
beta-homoAla-beta-homoLeu
pH 8.0, 25°C
Sphingosinicella microcystinivorans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
13144
-
alpha4beta4, 4 * 13144 + 4 * 25333, calculated from sequence
Sphingosinicella microcystinivorans
13168
-
alpha4beta4, 4 * 13168 + 4 * 25465, MALDI-TOF MS
Sphingosinicella microcystinivorans
25333
-
alpha4beta4, 4 * 13144 + 4 * 25333, calculated from sequence
Sphingosinicella microcystinivorans
25465
-
alpha4beta4, 4 * 13168 + 4 * 25465, MALDI-TOF MS
Sphingosinicella microcystinivorans
130000
-
gel filtration
Sphingosinicella microcystinivorans
130000
-
gel filtration
Sphingosinicella xenopeptidilytica
150200
-
electrospray ionisation mass spectrometry
Sphingosinicella xenopeptidilytica
155800
-
electrospray ionisation mass spectrometry
Sphingosinicella microcystinivorans
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
proteolytic modification
cleavage of the Y2 BapA preprotein between the conserved residues N275 and S276
Sphingosinicella microcystinivorans
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant
Sphingosinicella microcystinivorans
recombinant
Sphingosinicella xenopeptidilytica
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.2
-
pH 8.0, 25°C, substrate: beta-Ala-p-nitroanilide
Sphingosinicella microcystinivorans
0.62
-
pH 8.0, 25°C, substrate: beta-Ala-p-nitroanilide
Sphingosinicella xenopeptidilytica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
beta-Ala-Ile-beta-homoTyr + H2O
0.3% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica
beta-Ala + Ile-beta-homoTyr
-
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
1.5% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans
beta-Ala + Ile-beta-homoTyr
-
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
0.3% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica 3-2W4
beta-Ala + Ile-beta-homoTyr
-
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
1.5% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans Y2
beta-Ala + Ile-beta-homoTyr
-
-
-
?
beta-Ala-L-His + H2O
i.e. carnosine, 0.8% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica
beta-Ala + L-His
-
-
-
?
beta-Ala-L-His + H2O
i.e. carnosine, 2% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans
beta-Ala + L-His
-
-
-
?
beta-Ala-L-His + H2O
i.e. carnosine, 0.8% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica 3-2W4
beta-Ala + L-His
-
-
-
?
beta-Ala-L-His + H2O
i.e. carnosine, 2% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans Y2
beta-Ala + L-His
-
-
-
?
beta-Ala-p-nitroanilide + H2O
-
708467
Sphingosinicella xenopeptidilytica
beta-Ala + p-nitroaniline
-
-
-
?
beta-Ala-p-nitroanilide + H2O
-
708467
Sphingosinicella microcystinivorans
beta-Ala + p-nitroaniline
-
-
-
?
beta-Ala-p-nitroanilide + H2O
-
708467
Sphingosinicella xenopeptidilytica 3-2W4
beta-Ala + p-nitroaniline
-
-
-
?
beta-Ala-p-nitroanilide + H2O
-
708467
Sphingosinicella microcystinivorans Y2
beta-Ala + p-nitroaniline
-
-
-
?
beta-homoAla-beta-homoLeu + H2O
preferred substrate
708467
Sphingosinicella microcystinivorans
beta-homoAla-beta-homoLeu
-
-
-
?
beta-homoAla-beta-homoLeu + H2O
35% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica
beta-homoAla-beta-homoLeu
-
-
-
?
beta-homoAla-beta-homoLeu + H2O
35% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica 3-2W4
beta-homoAla-beta-homoLeu
-
-
-
?
beta-homoAla-beta-homoLeu + H2O
preferred substrate
708467
Sphingosinicella microcystinivorans Y2
beta-homoAla-beta-homoLeu
-
-
-
?
beta-homoArg-Ile-beta-homoTyr + H2O
0.2% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica
beta-homoArg + Ile-beta-homoTyr
-
-
-
?
beta-homoArg-Ile-beta-homoTyr + H2O
3.4% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans
beta-homoArg + Ile-beta-homoTyr
-
-
-
?
beta-homoGln-Ile-beta-homoTyr + H2O
0.2% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica
beta-homoGln + Ile-beta-homoTyr
-
-
-
?
beta-homoGln-Ile-beta-homoTyr + H2O
0.3% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans
beta-homoGln + Ile-beta-homoTyr
-
-
-
?
beta-homoHis-Ile-beta-homoTyr + H2O
0.3% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica
beta-homoHis + Ile-beta-homoTyr
-
-
-
?
beta-homoHis-Ile-beta-homoTyr + H2O
0.4% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans
beta-homoHis + Ile-beta-homoTyr
-
-
-
?
beta-homoLeu-Ile-beta-homoTyr + H2O
12% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans
beta-homoLeu + Ile-beta-homoTyr
-
-
-
?
beta-homoLeu-Ile-beta-homoTyr + H2O
61% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica
beta-homoLeu + Ile-beta-homoTyr
-
-
-
?
beta-homoLys-Ile-beta-homoTyr + H2O
0.5% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans
beta-homoLys + Ile-beta-homoTyr
-
-
-
?
beta-homoLys-Ile-beta-homoTyr + H2O
0.5% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica
beta-homoLys + Ile-beta-homoTyr
-
-
-
?
beta-homoPhe-Ile-beta-homoTyr + H2O
15% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans
beta-homoPhe + Ile-beta-homoTyr
-
-
-
?
beta-homoPhe-Ile-beta-homoTyr + H2O
22% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica
beta-homoPhe + Ile-beta-homoTyr
-
-
-
?
beta-homoSer-Ile-beta-homoTyr + H2O
13% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans
beta-homoSer + Ile-beta-homoTyr
-
-
-
?
beta-homoSer-Ile-beta-homoTyr + H2O
3% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica
beta-homoSer + Ile-beta-homoTyr
-
-
-
?
beta-homoThr-Ile-beta-homoTyr + H2O
1.6% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans
beta-homoThr + Ile-beta-homoTyr
-
-
-
?
beta-homoThr-Ile-beta-homoTyr + H2O
2% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica
beta-homoThr + Ile-beta-homoTyr
-
-
-
?
beta-homoTrp-Ile-beta-homoTyr + H2O
1.3% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans
beta-homoTrp + Ile-beta-homoTyr
-
-
-
?
beta-homoTrp-Ile-beta-homoTyr + H2O
1.5% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica
beta-homoTrp + Ile-beta-homoTyr
-
-
-
?
beta-homoTyr-Ile-beta-homoTyr + H2O
15% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica
beta-homoTyr + Ile-beta-homoTyr
-
-
-
?
beta-homoTyr-Ile-beta-homoTyr + H2O
7% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans
beta-homoTyr + Ile-beta-homoTyr
-
-
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
preferred substrate
708467
Sphingosinicella xenopeptidilytica
beta-homoVal + beta-homoAla-beta-homoLeu
-
-
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
27% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans
beta-homoVal + beta-homoAla-beta-homoLeu
-
-
-
?
beta-homoVal-Ile-beta-homoTyr + H2O
15% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans
beta-homoVal + Ile-beta-homoTyr
-
-
-
?
beta-homoVal-Ile-beta-homoTyr + H2O
32% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica
beta-homoVal + Ile-beta-homoTyr
-
-
-
?
D-beta-homoVal-Ile-beta-homoTyr + H2O
the rate of cleavage is slower by factor 28 as compared to the L-enantiomer. 0.5% of the activity with beta-homoAla-beta-homoLeu
708467
Sphingosinicella microcystinivorans
D-beta-homoVal + Ile-beta-homoTyr
-
-
-
?
D-beta-homoVal-Ile-beta-homoTyr + H2O
the rate of cleavage is slower by factor 35 as compared to the L-enantiomer. 0.9% of the activity with beta-homoVal-beta-homoAla-beta-homoLeu
708467
Sphingosinicella xenopeptidilytica
D-beta-homoVal + Ile-beta-homoTyr
-
-
-
?
additional information
the alpha-tripeptide Val-Ala-Leu and bestatin are not accepted as substrates. No degradation of DL-pyroglutamic acid-p-nitroanilide
708467
Sphingosinicella microcystinivorans
?
-
-
-
-
additional information
the alpha-tripeptide Val-Ala-Leu and bestatin are not accepted as substrates. No degradation of DL-pyroglutamyl-p-nitroanilide
708467
Sphingosinicella xenopeptidilytica
?
-
-
-
-
additional information
the alpha-tripeptide Val-Ala-Leu and bestatin are not accepted as substrates. No degradation of DL-pyroglutamyl-p-nitroanilide
708467
Sphingosinicella xenopeptidilytica 3-2W4
?
-
-
-
-
additional information
the alpha-tripeptide Val-Ala-Leu and bestatin are not accepted as substrates. No degradation of DL-pyroglutamic acid-p-nitroanilide
708467
Sphingosinicella microcystinivorans Y2
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
octamer
alpha4beta4, 4 * 13144 + 4 * 25333, calculated from sequence; alpha4beta4, 4 * 13168 + 4 * 25465, MALDI-TOF MS
Sphingosinicella microcystinivorans
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Sphingosinicella microcystinivorans
25
-
assay at
Sphingosinicella xenopeptidilytica
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
half-life: 1 h
Sphingosinicella microcystinivorans
60
-
24 h, stable
Sphingosinicella xenopeptidilytica
70
-
5 min, complete inactivation
Sphingosinicella microcystinivorans
70
-
half-life: 26 min
Sphingosinicella xenopeptidilytica
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.13
-
beta-Ala-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella microcystinivorans
0.75
-
beta-Ala-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
4.3
-
beta-homoVal-beta-homoAla-beta-homoLeu
pH 8.0, 25°C
Sphingosinicella microcystinivorans
6
-
beta-homoVal-beta-homoAla-beta-homoLeu
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
12
-
beta-homoAla-beta-homoLeu
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
60
-
beta-homoAla-beta-homoLeu
pH 8.0, 25°C
Sphingosinicella microcystinivorans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9
-
Sphingosinicella xenopeptidilytica
8
-
assay at
Sphingosinicella microcystinivorans
8
-
assay at
Sphingosinicella xenopeptidilytica
10
-
-
Sphingosinicella microcystinivorans
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.0085
-
beta-Ala-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
0.017
-
beta-Ala-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella microcystinivorans
0.029
-
beta-Ala-L-His
pH 8.0, 25°C
Sphingosinicella microcystinivorans
0.092
-
beta-Ala-L-His
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
0.11
-
beta-homoVal-beta-homoAla-beta-homoLeu
pH 8.0, 25°C
Sphingosinicella microcystinivorans
0.59
-
beta-homoAla-beta-homoLeu
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
0.67
-
beta-homoVal-beta-homoAla-beta-homoLeu
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
1.5
-
beta-homoAla-beta-homoLeu
pH 8.0, 25°C
Sphingosinicella microcystinivorans
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.0085
-
beta-Ala-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
0.017
-
beta-Ala-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella microcystinivorans
0.029
-
beta-Ala-L-His
pH 8.0, 25°C
Sphingosinicella microcystinivorans
0.092
-
beta-Ala-L-His
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
0.11
-
beta-homoVal-beta-homoAla-beta-homoLeu
pH 8.0, 25°C
Sphingosinicella microcystinivorans
0.59
-
beta-homoAla-beta-homoLeu
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
0.67
-
beta-homoVal-beta-homoAla-beta-homoLeu
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
1.5
-
beta-homoAla-beta-homoLeu
pH 8.0, 25°C
Sphingosinicella microcystinivorans
Other publictions for EC 3.4.11.25
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
753761
Kolesinska
-
Labeling and protecting N-ter ...
Sphingosinicella xenopeptidilytica
Helv. Chim. Acta
101
e1700259
2018
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
731203
Arima
Mutation of active site serine ...
Pseudomonas aeruginosa
Appl. Microbiol. Biotechnol.
98
1631-1640
2014
-
-
1
-
1
-
-
3
-
-
3
-
-
1
-
-
-
-
-
-
-
-
2
1
2
-
-
2
3
2
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
3
-
-
3
-
-
-
-
-
-
-
-
-
2
1
-
-
2
3
2
-
1
-
-
-
-
-
-
-
708080
Heck
Enzyme-catalyzed formation of ...
Sphingosinicella microcystinivorans, Sphingosinicella microcystinivorans Y2, Sphingosinicella xenopeptidilytica, Sphingosinicella xenopeptidilytica 3-2W4
Chem. Biodivers.
4
2016-2030
2007
-
2
-
-
-
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
708078
Heck
Enzymatic degradation of beta- ...
Sphingosinicella microcystinivorans, Sphingosinicella microcystinivorans Y2, Sphingosinicella xenopeptidilytica, Sphingosinicella xenopeptidilytica 3-2W4
Chem. Biodivers.
3
1325-1348
2006
-
-
-
-
-
-
-
6
-
-
-
-
-
9
-
-
2
-
-
-
-
-
60
-
2
2
-
-
6
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
2
-
-
-
-
60
-
2
-
-
6
2
-
-
-
-
-
-
-
6
6
708467
Geueke
Bacterial beta-peptidyl aminop ...
Sphingosinicella microcystinivorans, Sphingosinicella microcystinivorans Y2, Sphingosinicella xenopeptidilytica, Sphingosinicella xenopeptidilytica 3-2W4
FEBS J.
273
5261-5272
2006
-
-
-
-
-
-
4
6
-
-
8
-
-
9
-
1
2
-
-
-
2
-
46
1
2
2
-
4
6
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
6
-
-
8
-
-
-
1
2
-
-
2
-
46
1
2
-
4
6
4
-
-
-
-
-
-
-
8
8
708917
Geueke
A novel beta-peptidyl aminopep ...
Sphingosinicella xenopeptidilytica, Sphingosinicella xenopeptidilytica 3-2W4
J. Bacteriol.
187
5910-5917
2005
1
-
-
-
-
-
1
-
-
1
2
-
-
8
-
1
1
-
-
-
1
-
4
1
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
1
2
-
-
-
1
1
-
-
1
-
4
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-