Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.11.25 extracted from

  • Heck, T.; Limbach, M.; Geueke, B.; Zacharias, M.; Gardiner, J.; Kohler, H.P.; Seebach, D.
    Enzymatic degradation of beta- and mixed alpha,beta-oligopeptides (2006), Chem. Biodivers., 3, 1325-1348.
    View publication on PubMed
Search for more literature for 3.4.11.25

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
 beta-homoAla-p-nitroanilide pH 8.0, 25°C Sphingosinicella xenopeptidilytica
0.76
-
 beta-homoAla-p-nitroanilide pH 8.0, 25°C Sphingosinicella microcystinivorans
1.23
-
 beta-homoPhe-p-nitroanilide pH 8.0, 25°C Sphingosinicella xenopeptidilytica
1.45
-
 beta-homoPhe-p-nitroanilide pH 8.0, 25°C Sphingosinicella microcystinivorans
4.4
-
 beta-Ala-p-nitroanilide pH 8.0, 25°C Sphingosinicella microcystinivorans
8.1
-
 beta-Ala-p-nitroanilide pH 8.0, 25°C Sphingosinicella xenopeptidilytica

Organism

Organism UniProt Comment Textmining
Sphingosinicella microcystinivorans A0MTQ2
-
-
Sphingosinicella microcystinivorans Y2 A0MTQ2
-
-
Sphingosinicella xenopeptidilytica Q52VH2
-
-
Sphingosinicella xenopeptidilytica 3-2W4 Q52VH2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sphingosinicella xenopeptidilytica
-
 Sphingosinicella microcystinivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O completely degraded to the corresponding beta-homoamino acids within 312 h Sphingosinicella xenopeptidilytica beta-homoAla + beta-homoLys + beta-homoPhe
-
 ?
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O completely degraded to the corresponding beta-homoamino acids within 312 h Sphingosinicella microcystinivorans beta-homoAla + beta-homoLys + beta-homoPhe
-
 ?
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O completely degraded to the corresponding beta-homoamino acids within 312 h Sphingosinicella xenopeptidilytica 3-2W4 beta-homoAla + beta-homoLys + beta-homoPhe
-
 ?
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O completely degraded to the corresponding beta-homoamino acids within 312 h Sphingosinicella microcystinivorans Y2 beta-homoAla + beta-homoLys + beta-homoPhe
-
 ?
beta-Ala-Ile-beta-homoTyr + H2O hydrolysis at 0.3% compared to hydrolysis of beta-homoVal-Ile-Tyr Sphingosinicella xenopeptidilytica beta-Ala + Ile-beta-homoTyr
-
 ?
beta-Ala-Ile-beta-homoTyr + H2O hydrolysis at 1.5% compared to hydrolysis of beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-Ala + Ile-beta-homoTyr
-
 ?
beta-Ala-Ile-beta-homoTyr + H2O hydrolysis at 0.3% compared to hydrolysis of beta-homoVal-Ile-Tyr Sphingosinicella xenopeptidilytica 3-2W4 beta-Ala + Ile-beta-homoTyr
-
 ?
beta-Ala-Ile-beta-homoTyr + H2O hydrolysis at 1.5% compared to hydrolysis of beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans Y2 beta-Ala + Ile-beta-homoTyr
-
 ?
beta-Ala-L-His + H2O i.e. carnosine. Hydrolysis at 0.7% compared to hydrolysis of beta-homoVal-Ile-Tyr Sphingosinicella xenopeptidilytica beta-Ala + L-His
-
 ?
beta-Ala-L-His + H2O i.e. carnosine. Hydrolysis at 2% compared to hydrolysis of beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-Ala + L-His
-
 ?
beta-Ala-L-His + H2O i.e. carnosine. Hydrolysis at 0.7% compared to hydrolysis of beta-homoVal-Ile-Tyr Sphingosinicella xenopeptidilytica 3-2W4 beta-Ala + L-His
-
 ?
beta-Ala-L-His + H2O i.e. carnosine. Hydrolysis at 2% compared to hydrolysis of beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans Y2 beta-Ala + L-His
-
 ?
beta-Ala-p-nitroanilide + H2O
-
 Sphingosinicella xenopeptidilytica beta-Ala + p-nitroaniline
-
 ?
beta-Ala-p-nitroanilide + H2O
-
 Sphingosinicella microcystinivorans beta-Ala + p-nitroaniline
-
 ?
beta-Ala-p-nitroanilide + H2O
-
 Sphingosinicella xenopeptidilytica 3-2W4 beta-Ala + p-nitroaniline
-
 ?
beta-Ala-p-nitroanilide + H2O
-
 Sphingosinicella microcystinivorans Y2 beta-Ala + p-nitroaniline
-
 ?
beta-homoAla-beta-homoLeu + H2O preferred substrate Sphingosinicella microcystinivorans beta-homoAla + beta-homoLeu
-
 ?
beta-homoAla-beta-homoLeu + H2O hydrolysis at 31% compared to hydrolysis of beta-homoVal-Ile-Tyr Sphingosinicella xenopeptidilytica beta-homoAla + beta-homoLeu
-
 ?
beta-homoAla-p-nitroanilide + H2O
-
 Sphingosinicella xenopeptidilytica beta-homoAla + p-nitroaniline
-
 ?
beta-homoAla-p-nitroanilide + H2O
-
 Sphingosinicella microcystinivorans beta-homoAla + p-nitroaniline
-
 ?
beta-homoArg-Ile-beta-homoTyr + H2O hydrolysis at 0.2% compared to hydrolysis of beta-homoVal-Ile-Tyr Sphingosinicella xenopeptidilytica beta-homoArg + Ile-beta-homoTyr
-
 ?
beta-homoArg-Ile-beta-homoTyr + H2O hydrolysis at 0.35% compared to hydrolysis of beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-homoArg + Ile-beta-homoTyr
-
 ?
beta-homoGln-Ile-beta-homoTyr + H2O hydrolysis at 0.2% compared to hydrolysis of beta-homoVal-Ile-Tyr Sphingosinicella xenopeptidilytica beta-homoGln + Ile-beta-homoTyr
-
 ?
beta-homoGln-Ile-beta-homoTyr + H2O hydrolysis at 0.25% compared to hydrolysis of beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-homoGln + Ile-beta-homoTyr
-
 ?
beta-homoHis-Ile-beta-homoTyr + H2O hydrolysis at 0.2% compared to hydrolysis of beta-homoVal-Ile-Tyr Sphingosinicella xenopeptidilytica beta-homoHis + Ile-beta-homoTyr
-
 ?
beta-homoHis-Ile-beta-homoTyr + H2O hydrolysis at 0.35% compared to hydrolysis of beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-homoHis + Ile-beta-homoTyr
-
 ?
beta-homoLeu-Ile-beta-homoTyr + H2O hydrolysis at 12% compared to hydrolysis of beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-homoLeu + Ile-beta-homoTyr
-
 ?
beta-homoLeu-Ile-beta-homoTyr + H2O hydrolysis at 54% compared to hydrolysis of beta-homoVal-Ile-Tyr Sphingosinicella xenopeptidilytica beta-homoLeu + Ile-beta-homoTyr
-
 ?
beta-homoLys-Ile-beta-homoTyr + H2O hydrolysis at 0.48% compared to hydrolysis of beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-homoLys + Ile-beta-homoTyr
-
 ?
beta-homoLys-Ile-beta-homoTyr + H2O hydrolysis at 0.5% compared to hydrolysis of beta-homoVal-Ile-Tyr Sphingosinicella xenopeptidilytica beta-homoLys + Ile-beta-homoTyr
-
 ?
beta-homoPhe-Ile-beta-homoTyr + H2O hydrolysis at 15% compared to hydrolysis of beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-homoPhe + Ile-beta-homoTyr
-
 ?
beta-homoPhe-Ile-beta-homoTyr + H2O hydrolysis at 19% compared to hydrolysis of beta-homoVal-Ile-Tyr Sphingosinicella xenopeptidilytica beta-homoPhe + Ile-beta-homoTyr
-
 ?
beta-homoPhe-p-nitroanilide + H2O
-
 Sphingosinicella xenopeptidilytica beta-homoPhe + p-nitroaniline
-
 ?
beta-homoPhe-p-nitroanilide + H2O
-
 Sphingosinicella microcystinivorans beta-homoPhe + p-nitroaniline
-
 ?
beta-homoSer-Ile-beta-homoTyr + H2O hydrolysis at 12% compared to hydrolysis of beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-homoSer + Ile-beta-homoTyr
-
 ?
beta-homoSer-Ile-beta-homoTyr + H2O hydrolysis at 2.7% compared to hydrolysis of beta-homoVal-Ile-Tyr Sphingosinicella xenopeptidilytica beta-homoSer + Ile-beta-homoTyr
-
 ?
beta-homoThr-Ile-beta-homoTyr + H2O hydrolysis at 1.6% compared to hydrolysis of beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-homoThr + Ile-beta-homoTyr
-
 ?
beta-homoThr-Ile-beta-homoTyr + H2O hydrolysis at 1.9% compared to hydrolysis of beta-homoVal-Ile-Tyr Sphingosinicella xenopeptidilytica beta-homoThr + Ile-beta-homoTyr
-
 ?
beta-homoTrp-Ile-beta-homoTyr + H2O hydrolysis at 1.2% compared to hydrolysis of beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-homoTrp + Ile-beta-homoTyr
-
 ?
beta-homoTrp-Ile-beta-homoTyr + H2O hydrolysis at 1.3% compared to hydrolysis of beta-homoVal-Ile-Tyr Sphingosinicella xenopeptidilytica beta-homoTrp + Ile-beta-homoTyr
-
 ?
beta-homoTyr-Ile-beta-homoTyr + H2O hydrolysis at 13% compared to hydrolysis of beta-homoVal-Ile-Tyr Sphingosinicella xenopeptidilytica beta-homoTyr + Ile-beta-homoTyr
-
 ?
beta-homoTyr-Ile-beta-homoTyr + H2O hydrolysis at 7% compared to hydrolysis of beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-homoTyr + Ile-beta-homoTyr
-
 ?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O hydrolysis at 27% compared to hydrolysis of beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-homoVal + beta-homoAla-beta-homoLeu
-
 ?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O hydrolysis at 89% compared to hydrolysis of beta-homoVal-Ile-Tyr Sphingosinicella xenopeptidilytica beta-homoVal + beta-homoAla-beta-homoLeu
-
 ?
beta-homoVal-beta-homoArg-beta-homoArg + H2O hydrolysis at 0.2% compared to hydrolysis of beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-homoVal + beta-homoArg-beta-homoArg
-
 ?
beta-homoVal-beta-homoArg-beta-homoArg + H2O hydrolysis at 1.4% compared to hydrolysis of beta-homoVal-Ile-Tyr Sphingosinicella xenopeptidilytica beta-homoVal + beta-homoArg-beta-homoArg
-
 ?
beta-homoVal-beta-homoIle-beta-homoTyr + H2O completely hydrolyzed. Hydrolysis at 15% compared to hydrolysis of beta-homoVal-Ile-Tyr Sphingosinicella xenopeptidilytica beta-homoVal + beta-homoIle + beta-homoTyr
-
 ?
beta-homoVal-beta-homoIle-beta-homoTyr + H2O completely hydrolyzed. Hydrolysis at 5% compared to hydrolysis of beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-homoVal + beta-homoIle + beta-homoTyr
-
 ?
beta-homoVal-beta-homoIle-Tyr + H2O completely hydrolyzed. Hydrolysis at 20% compared to hydrolysis of beta-(3-homoVal)-Ile-Tyr Sphingosinicella xenopeptidilytica beta-homoVal + beta-homoIle + Tyr
-
 ?
beta-homoVal-beta-homoIle-Tyr + H2O completely hydrolyzed. Hydrolysis at 5% compared to hydrolysis of beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-homoVal + beta-homoIle + Tyr
-
 ?
beta-homoVal-Ile-beta-homoTyr + H2O only the N-terminal beta-homoVal is released. Hydrolysis at 15% compared to hydrolysis of beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-homoVal + Ile-beta-homoTyr
-
 ?
beta-homoVal-Ile-beta-homoTyr + H2O only the N-terminal beta-homoVal is released. Hydrolysis at 28% compared to hydrolysis of beta-homoVal-Ile-Tyr Sphingosinicella xenopeptidilytica beta-homoVal + Ile-beta-homoTyr
-
 ?
beta-homoVal-Ile-Tyr + H2O only the N-terminal beta-homoVal is released. Hydrolysis at 32% compared to hydrolysis of beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans beta-homoVal + Ile-Tyr
-
 ?
D-beta-homoVal-Ile-beta-homoTyr + H2O hydrolysis at 0.5% compared to hydrolysis of beta-homoAla-beta-homoLeu Sphingosinicella microcystinivorans D-beta-homoVal + Ile-beta-homoTyr
-
 ?
D-beta-homoVal-Ile-beta-homoTyr + H2O hydrolysis at 0.8% compared to hydrolysis of beta-homoVal-Ile-Tyr Sphingosinicella xenopeptidilytica D-beta-homoVal + Ile-beta-homoTyr
-
 ?
additional information all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded Sphingosinicella xenopeptidilytica ?
-
 ?
additional information all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded Sphingosinicella microcystinivorans ?
-
 ?
additional information all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded Sphingosinicella xenopeptidilytica 3-2W4 ?
-
 ?
additional information all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded Sphingosinicella microcystinivorans Y2 ?
-
 ?
Val-Ile-Tyr + H2O only the N-terminal beta-homoVal is released. Preferred substrate Sphingosinicella xenopeptidilytica beta-homoVal + Ile-Tyr
-
 ?

Synonyms

Synonyms Comment Organism
3-2W4 BapA
-
 Sphingosinicella xenopeptidilytica
Y2 BapA
-
 Sphingosinicella microcystinivorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Sphingosinicella xenopeptidilytica
25
-
 assay at Sphingosinicella microcystinivorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.13
-
 beta-Ala-p-nitroanilide pH 8.0, 25°C Sphingosinicella microcystinivorans
0.22
-
 beta-homoPhe-p-nitroanilide pH 8.0, 25°C Sphingosinicella microcystinivorans
0.75
-
 beta-Ala-p-nitroanilide pH 8.0, 25°C Sphingosinicella xenopeptidilytica
2.89
-
 beta-homoAla-p-nitroanilide pH 8.0, 25°C Sphingosinicella microcystinivorans
13.2
-
 beta-homoAla-p-nitroanilide pH 8.0, 25°C Sphingosinicella xenopeptidilytica
30.2
-
 beta-homoPhe-p-nitroanilide pH 8.0, 25°C Sphingosinicella xenopeptidilytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Sphingosinicella xenopeptidilytica
8
-
 assay at Sphingosinicella microcystinivorans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.029
-
 beta-Ala-p-nitroanilide pH 8.0, 25°C Sphingosinicella microcystinivorans
0.092
-
 beta-Ala-p-nitroanilide pH 8.0, 25°C Sphingosinicella xenopeptidilytica
0.154
-
 beta-homoPhe-p-nitroanilide pH 8.0, 25°C Sphingosinicella microcystinivorans
3.8
-
 beta-homoAla-p-nitroanilide pH 8.0, 25°C Sphingosinicella microcystinivorans
18.7
-
 beta-homoAla-p-nitroanilide pH 8.0, 25°C Sphingosinicella xenopeptidilytica
24.6
-
 beta-homoPhe-p-nitroanilide pH 8.0, 25°C Sphingosinicella xenopeptidilytica