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Literature summary for 3.4.11.22 extracted from

  • Cortes, L.; Carvalho, A.L.; Todo-Bom, A.; Faro, C.; Pires, E.; Verissimo, P.
    Purification of a novel aminopeptidase from the pollen of Parietaria judaica that alters epithelial integrity and degrades neuropeptides (2006), J. Allergy Clin. Immunol., 118, 878-884.
    View publication on PubMed
Search for more literature for 3.4.11.22

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 3% residual activity at 10 mM Parietaria judaica
amastatin 1.5% residual activity at 0.01 mM Parietaria judaica
bestatin 2% residual activity at 0.01 mM Parietaria judaica
Ca2+ 76% residual activity at 1 mM Parietaria judaica
chymostatin 39% residual activity at 0.1 mM Parietaria judaica
EDTA 1.5% residual activity at 5 mM Parietaria judaica
Mg2+ 71% residual activity at 1 mM Parietaria judaica
Mn2+ complete inhibition at 1 mM Parietaria judaica
additional information not inhibited by pepstatin, L-trans-epoxysuccinyl-leucylamide-(4-guanidino)-butane, alpha1-antitrypsin inhibitor or aprotinin Parietaria judaica
N-p-tosyl-L-lysine chloromethyl ketone 64% residual activity at 0.1 mM Parietaria judaica
N-p-tosyl-L-phenylalanine chloromethyl ketone 8% residual activity at 0.1 mM Parietaria judaica
Phenylmethylsulfonylfluoride 6% residual activity at 1 mM Parietaria judaica
Zn2+ 2% residual activity at 1 mM Parietaria judaica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 L-Ala-p-nitroanilide in 50 mM Tris-HCl, pH 8.1, at 30°C Parietaria judaica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
98000
-
 SDS-PAGE Parietaria judaica

Organism

Organism UniProt Comment Textmining
Parietaria judaica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sephacel column chromatography, ammonium sulfate precipitation, Superdex 200 gel filtration, HiTrap Q column chromatography, and arginine-Sepharose column chromatography Parietaria judaica

Source Tissue

Source Tissue Comment Organism Textmining
pollen
-
 Parietaria judaica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.16
-
 pollen extract, in 50 mM Tris-HCl, pH 8.1, at 30°C Parietaria judaica
4.88
-
 after 30.5fold purification, in 50 mM Tris-HCl, pH 8.1, at 30°C Parietaria judaica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DRVYIHPFHL + H2O angiotensin I Parietaria judaica L-Asp + L-Arg + VYIHPFHL
-
 ?
E-cadherin + H2O
-
 Parietaria judaica ?
-
 ?
HSDAVFTDNYTRLRKQMAVKKYLNSILN + H2O protein VIP Parietaria judaica His + SDAVFTDNYTRLRKQM + AVKKYLN + SILN
-
 ?
L-Ala-p-nitroanilide + H2O preferred substrate Parietaria judaica L-Ala + p-nitroaniline
-
 ?
L-Arg-p-nitroanilide + H2O
-
 Parietaria judaica L-Arg + p-nitroaniline
-
 ?
additional information L-Val-p-nitroanilide, L-Leu-p-nitroanilide, L-Ala-Ala-Ala-p-nitroanilide, N-succinyl-Ala-Ala-Val-p-nitroanilide, N-succinyl-Ala-Ala-Pro-Phe-p-nitroanilide, benzoyl-Phe-Val-Arg-p-nitroanilide, and benzoyl-Arg-p-nitroanilide are no substrates Parietaria judaica ?
-
 ?
occludin + H2O
-
 Parietaria judaica ?
-
 ?
RPKPQQFFGLM + H2O substance P Parietaria judaica RPKPQ + QFF + GLM
-
 ?
RPPGFSPFR + H2O bradykinin Parietaria judaica Arg + PPGF + SPFR
-
 ?

Synonyms

Synonyms Comment Organism
Pj-peptidase
-
 Parietaria judaica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Parietaria judaica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 60 40% activity at 25°C, no activity above 60°C Parietaria judaica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Parietaria judaica

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5 9
-
 Parietaria judaica