KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of peptide trimming, overview | Sus scrofa | |
0.09 | - |
AYWANATRSGA | pH 7.5, 37°C | Sus scrofa | |
0.111 | - |
QLESIINFEKY | pH 7.5, 37°C | Sus scrofa | |
0.124 | - |
QLESIINFEKL | pH 7.5, 37°C | Sus scrofa | |
0.124 | - |
QLESIINFEKA | pH 7.5, 37°C | Sus scrofa | |
0.138 | - |
QLESIINFEKL-amide | pH 7.5, 37°C | Sus scrofa | |
0.148 | - |
EFAPGNYPAL | pH 7.5, 37°C | Sus scrofa | |
0.253 | - |
AYWANATRSG-D-Ala | pH 7.5, 37°C | Sus scrofa | |
0.7 | - |
QLESIINFEKD | pH 7.5, 37°C | Sus scrofa | |
0.8 | - |
QLESIINFEKK | pH 7.5, 37°C | Sus scrofa | |
0.91 | - |
QLESIINFEKR | pH 7.5, 37°C | Sus scrofa | |
1.325 | - |
EFAPGNYPAD | pH 7.5, 37°C | Sus scrofa | |
1.5 | - |
EFAPGNYPAK | pH 7.5, 37°C | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | isozymes ERAP1 and ERAP2 | Sus scrofa | 5783 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Sus scrofa | the endoplasmic reticulum enzyme ERAP1 trims precursors to lengths of MHC class I peptides by a 'molecular ruler' mechanism, overview, the enzyme is important in antigen presentation, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
isozyme ERAP1 and ERAP2 | - |
Purification (Comment) | Organism |
---|---|
further purification of commercial kidney enzyme preparation by affinity and hydrophobic interaction chromatography | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | from kidney, isozyme ERAP1 | Sus scrofa | - |
kidney | isozyme ERAP1 | Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
AAEAAG-NH2 + H2O | - |
Sus scrofa | L-Ala + AEAAG-NH2 | - |
? | |
AAVVAAG-NH2 + H2O | - |
Sus scrofa | L-Ala + AVVAAG-NH2 | - |
? | |
AEAA-NH2 + H2O | - |
Sus scrofa | L-Ala + EAA-NH2 | - |
? | |
AYWANATRSG-D-Ala + H2O | - |
Sus scrofa | L-Ala + AYWANATRSG-D-Ala | - |
? | |
AYWANATRSGA + H2O | high activity compared to other peptide substrates | Sus scrofa | L-Ala + YWANATRSGA | - |
? | |
EAA-NH2 + H2O | - |
Sus scrofa | L-Glu + AA-NH2 | - |
? | |
EFAPGNYPAD + H2O | low activity compared to other peptide substrates | Sus scrofa | L-Glu + FAPGNYPAD | - |
? | |
EFAPGNYPAK + H2O | low activity compared to other peptide substrates | Sus scrofa | L-Glu + FAPGNYPAK | - |
? | |
EFAPGNYPAL + H2O | high activity compared to other peptide substrates | Sus scrofa | L-Glu + FAPGNYPAL | - |
? | |
additional information | the endoplasmic reticulum enzyme ERAP1 trims precursors to lengths of MHC class I peptides by a 'molecular ruler' mechanism, overview, the enzyme is important in antigen presentation, overview | Sus scrofa | ? | - |
? | |
additional information | L-amino acid peptide substrate specificity and strongly preferred chain length of 9-16 residues of isozyme ERAP1, overview, no activity with N-acetylQLESIINFEKL | Sus scrofa | ? | - |
? | |
QLESIINFEK + H2O | low activity compared to other peptide substrates | Sus scrofa | L-Gln + LESIINFEK | - |
? | |
QLESIINFEKA + H2O | high activity compared to other peptide substrates | Sus scrofa | L-Gln + LESIINFEKA | - |
? | |
QLESIINFEKD + H2O | low activity compared to other peptide substrates | Sus scrofa | L-Gln + LESIINFEKD | - |
? | |
QLESIINFEKK + H2O | low activity compared to other peptide substrates | Sus scrofa | L-Gln + LESIINFEKK | - |
? | |
QLESIINFEKL + H2O | best peptide substrate | Sus scrofa | L-Gln + LESIINFEKL | - |
? | |
QLESIINFEKL-amide + H2O | high activity compared to other peptide substrates | Sus scrofa | L-Gln + LESIINFEKL-amide | - |
? | |
QLESIINFEKR + H2O | low activity compared to other peptide substrates | Sus scrofa | L-Gln + LESIINFEKR | - |
? | |
QLESIINFEKY + H2O | high activity compared to other peptide substrates | Sus scrofa | L-Gln + LESIINFEKY | - |
? | |
QLESIINFELK + H2O | - |
Sus scrofa | L-Gln + LESIINFELK | - |
? | |
SIINFEKL + H2O | - |
Sus scrofa | L-Ser + IINFEKL | - |
? | |
TTQRTRALV-NH2 + H2O | - |
Sus scrofa | L-Thr + TQRTRALV-NH2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aminopeptidase I | - |
Sus scrofa |
ER aminopeptidase | - |
Sus scrofa |
ERAP1 | - |
Sus scrofa |
leucine aminopeptidase | - |
Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Sus scrofa |