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Literature summary for 3.4.11.22 extracted from

  • Desimone, M.; Kruger, M.; Wessel, T.; Wehofsky, M.; Hoffmann, R.; Wagner, E.
    Purification and characterization of an aminopeptidase from the chloroplast stroma of barley leaves by chromatographic and electrophoretic methods (2000), J. Chromatogr. B, 737, 285-293.
    View publication on PubMed
Search for more literature for 3.4.11.22

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline complete inhibition Hordeum vulgare
amastatin strong inhibition Hordeum vulgare
bestatin strong inhibition Hordeum vulgare

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast stroma
-
 Hordeum vulgare 9570
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information metallopeptidase Hordeum vulgare

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
84000
-
 1 * 84000, SDS-PAGE Hordeum vulgare
93000
-
 gel filtration Hordeum vulgare

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native soluble enzyme from chloroplast stroma to homogeneity by chlorplast preparation, ammonium sulfate fractionation, hydrophobic interaction chromatography, gel filtration, and ion exchange chromatography Hordeum vulgare

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Hordeum vulgare
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-4-nitroanilide + H2O
-
 Hordeum vulgare Gly + 4-nitroaniline
-
 ?
L-Ala-4-nitroanilide + H2O
-
 Hordeum vulgare L-Ala + 4-nitroaniline
-
 ?
L-Arg-4-nitroanilide + H2O
-
 Hordeum vulgare L-Arg + 4-nitroaniline
-
 ?
L-Leu-4-nitroanilide + H2O
-
 Hordeum vulgare L-Leu + 4-nitroaniline
-
 ?
L-Lys-4-nitroanilide + H2O
-
 Hordeum vulgare L-Lys + 4-nitroaniline
-
 ?
L-Met-4-nitroanilide + H2O
-
 Hordeum vulgare L-Met + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 84000, SDS-PAGE Hordeum vulgare