Application | Comment | Organism |
---|---|---|
nutrition | the enzyme is useful to reduce bitterness of food products by hydrolysis of peptides with hydrophobic amino acids causing the bitter taste | Grifola frondosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | inactivation | Grifola frondosa | |
bestatin | strong inhibition | Grifola frondosa | |
Co2+ | 66% inhibition at 0.03 mM | Grifola frondosa | |
Cu2+ | 32% inhibition at 0.03 mM | Grifola frondosa | |
EDTA | inactivation | Grifola frondosa | |
Mg2+ | complete inhibition at 0.03 mM | Grifola frondosa | |
Mn2+ | 94% inhibition at 0.03 mM | Grifola frondosa | |
additional information | no inhibition by DFP | Grifola frondosa | |
Zn2+ | 42% inhibition at 0.03 mM | Grifola frondosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
L-Val-4-nitroanilide | pH 8.5, 65°C | Grifola frondosa | |
0.31 | - |
L-Phe-4-nitroanilide | pH 8.5, 65°C | Grifola frondosa | |
0.35 | - |
L-Leu-4-nitroanilide | pH 8.5, 65°C | Grifola frondosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is no metallopeptidase | Grifola frondosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
gel filtration | Grifola frondosa |
30000 | - |
1 * 30000, SDS-PAGE | Grifola frondosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Grifola frondosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 178fold to homogeneity by ammonium sulfate fractionation, hydrophobic interaction chromatography, gel filtration, and ion exchange chromatography | Grifola frondosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.55 | - |
purified enzyme | Grifola frondosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Leu-4-nitroanilide + H2O | best substrate | Grifola frondosa | L-Leu + 4-nitroaniline | - |
? | |
L-Phe-4-nitroanilide + H2O | - |
Grifola frondosa | L-Phe + 4-nitroaniline | - |
? | |
L-Val-4-nitroanilide + H2O | low activity | Grifola frondosa | L-Val + 4-nitroaniline | - |
? | |
additional information | the enzyme prefers peptide substrates with an N-terminal hydrophobic amino acid residue | Grifola frondosa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000, SDS-PAGE | Grifola frondosa |
Synonyms | Comment | Organism |
---|---|---|
Aminopeptidase | - |
Grifola frondosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Grifola frondosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
60 min, purified enzyme, stable | Grifola frondosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
L-Val-4-nitroanilide | pH 8.5, 65°C | Grifola frondosa | |
0.95 | - |
L-Phe-4-nitroanilide | pH 8.5, 65°C | Grifola frondosa | |
1.43 | - |
L-Leu-4-nitroanilide | pH 8.5, 65°C | Grifola frondosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Grifola frondosa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10.5 | 60 min, purified enzyme, stable | Grifola frondosa |