Literature summary for 3.4.11.22 extracted from

Desmarais, W.T.; Bienvenue, D.L.; Bzymek, K.P.; Holz, R.C.; Petsko, G.A.; Ringe, D.
The 1.20 A resolution crystal structure of the aminopeptidase from Aeromonas proteolytica complexed with tris: a tale of buffer inhibition (2002), Structure, 10, 1063-1072.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
16 mg/ml enzyme complexed with inhibitory Tris, in 10 mM Tris, pH 8.0, 10 mM KSCN, 0.4 NaCl, vapour diffusion method, with precipitant solution containing 100 mM Tris, pH 8.0, 100 mM KSCN, 4.5 M NaCl, 48 h, X-ray diffraction structure determination and analysis at 1.2 A resolution	Vibrio proteolyticus

Inhibitors

Inhibitors	Comment	Organism	Structure
Tris	chelated to active site Zn2+	Vibrio proteolyticus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	bound in the active site, binding structure, bridged bimetallic enzyme	Vibrio proteolyticus

Organism

Organism	UniProt	Comment	Textmining
Vibrio proteolyticus	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
Release of an N-terminal amino acid, preferably a neutral or hydrophobic one, from a polypeptide. Aminoacyl-arylamides are poor substrates	active site structure, reaction mechanism	Vibrio proteolyticus	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
commercial preparation	-	Vibrio proteolyticus	-

Synonyms

Synonyms	Comment	Organism
AAP	-	Vibrio proteolyticus
Aeromonas proteolytica aminopeptidase	-	Vibrio proteolyticus
More	see also EC 3.4.11.10	Vibrio proteolyticus

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Release 2023.1 (January 2023)