Literature summary for 3.4.11.22 extracted from

Kuo, L.Y.; Hwang, G.Y.; Lai, Y.J.; Yang, S.L.; Lin, L.L.
Overexpression, purification, and characterization of the recombinant leucine aminopeptidase II of Bacillus stearothermophilus (2003), Curr. Microbiol., 47, 40-45.

Search for more literature for 3.4.11.22

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli as His-tagged enzyme	Geobacillus stearothermophilus

Inhibitors

Inhibitors	Comment	Organism	Structure
Ca2+	slight inhibition	Geobacillus stearothermophilus
Cu2+	complete inhibition at 1 mM, recombinant enzyme	Geobacillus stearothermophilus
DTT	complete inhibition at 10 mM	Geobacillus stearothermophilus
EDTA	complete inhibition at 10 mM, Co2+ reactivates, only slight reactivation by Ca2+, Mn2+ and Mg2+, recombinant enzyme	Geobacillus stearothermophilus
Hg2+	99% inhibition at 1 mM, recombinant enzyme	Geobacillus stearothermophilus
Mn2+	73% inhibition at 1 mM	Geobacillus stearothermophilus
additional information	not affected by Mg2+	Geobacillus stearothermophilus
Ni2+	89% inhibition at 1 mM	Geobacillus stearothermophilus
PMSF	complete inhibition at 10 mM	Geobacillus stearothermophilus
Zn2+	94% inhibition at 1 mM	Geobacillus stearothermophilus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	activates 3.8fold at 1 mM, dependent on, recombinant enzyme	Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
44500	-	x * 44500, recombinant enzyme, SDS-PAGE	Geobacillus stearothermophilus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	-	-	-
Geobacillus stearothermophilus NIB 8924	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli, 9.2fold to homogeneity	Geobacillus stearothermophilus

Reaction

Reaction	Comment	Organism	Reaction ID
Release of an N-terminal amino acid, preferably a neutral or hydrophobic one, from a polypeptide. Aminoacyl-arylamides are poor substrates	cobalt-dependent exopeptidase	Geobacillus stearothermophilus	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
425	-	purified recombinant enzyme	Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Arg-4-nitroanilide + H2O	-	Geobacillus stearothermophilus	L-Arg + 4-nitroaniline	-	?
L-Arg-4-nitroanilide + H2O	-	Geobacillus stearothermophilus NIB 8924	L-Arg + 4-nitroaniline	-	?
L-Leu-4-nitroanilide + H2O	preferred substrate, recombinant enzyme	Geobacillus stearothermophilus	L-Leu + 4-nitroaniline	-	?
L-Leu-4-nitroanilide + H2O	preferred substrate, recombinant enzyme	Geobacillus stearothermophilus NIB 8924	L-Leu + 4-nitroaniline	-	?
L-Lys-4-nitroanilide + H2O	-	Geobacillus stearothermophilus	L-Lys + 4-nitroaniline	-	?
L-Lys-4-nitroanilide + H2O	-	Geobacillus stearothermophilus NIB 8924	L-Lys + 4-nitroaniline	-	?

Subunits

Subunits	Comment	Organism
?	x * 44500, recombinant enzyme, SDS-PAGE	Geobacillus stearothermophilus

Synonyms

Synonyms	Comment	Organism
LAP II	-	Geobacillus stearothermophilus
leucine aminopeptidase II	-	Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	recombinant enzyme	Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	recombinant enzyme	Geobacillus stearothermophilus

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Release 2023.1 (January 2023)