Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, functional overexpression in Fusarium venenatum and Aspergillus oryzae | Aspergillus oryzae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10% inhibition at 1 mM | Aspergillus oryzae | |
o-phenanthroline | 91% inhibition at 1 mM | Aspergillus oryzae | |
PMSF | 5% inhibition at 1 mM | Aspergillus oryzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
Glu-4-nitroanilide | pH 7.5, 21°C, recombinant enzyme | Aspergillus oryzae | |
3.5 | - |
Ala-4-nitroanilide | pH 7.5, 21°C, recombinant enzyme | Aspergillus oryzae | |
7 | - |
Leu-4-nitroanilide | pH 7.5, 21°C, recombinant enzyme | Aspergillus oryzae | |
9 | - |
Lys-4-nitroanilide | pH 7.5, 21°C, recombinant enzyme | Aspergillus oryzae | |
13 | - |
Val-4-nitroanilide | pH 7.5, 21°C, recombinant enzyme | Aspergillus oryzae | |
15 | - |
L-Pro-4-nitroanilide | pH 7.5, 21°C, recombinant enzyme | Aspergillus oryzae | |
51 | - |
Ile-4-nitroanilide | pH 7.5, 21°C, recombinant enzyme | Aspergillus oryzae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secretion to the culture medium | Aspergillus oryzae | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required, metalloenzyme, bound at the active site, enzyme contains the HEXXH motif | Aspergillus oryzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51950 | - |
x * 56000, deglycosylated recombinant enzyme, SDS-PAGE, x * 51950, mature enzyme, amino acid sequence calculation | Aspergillus oryzae |
56000 | - |
x * 56000, deglycosylated recombinant enzyme, SDS-PAGE, x * 51950, mature enzyme, amino acid sequence calculation | Aspergillus oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
strain ATCC 20386 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | N-glycosylation | Aspergillus oryzae |
Purification (Comment) | Organism |
---|---|
from culture supernatant | Aspergillus oryzae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
reaction velocity in descending order with Xaa being Pro, Ala, Leu, Gly, and Glu | Aspergillus oryzae |
4.12 | - |
with Leu-4-nitroanilide as substrate, measurement of absorption | Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-4-nitroanilide + H2O | - |
Aspergillus oryzae | Ala + 4-nitroaniline | - |
? | |
Ala-Ala-Pro-Tyr-Lys-amide + H2O | - |
Aspergillus oryzae | Ala + Ala-Pro-Tyr-Lys-amide | - |
? | |
Glu-4-nitroanilide + H2O | - |
Aspergillus oryzae | Glu + 4-nitroaniline | - |
? | |
Glu-Ala-Pro-Tyr-Lys-amide + H2O | - |
Aspergillus oryzae | Glu + Ala-Pro-Tyr-Lys-amide | - |
? | |
Gly-Ala-Pro-Tyr-Lys-amide + H2O | - |
Aspergillus oryzae | Gly + Ala-Pro-Tyr-Lys-amide | - |
? | |
Ile-4-nitroanilide + H2O | - |
Aspergillus oryzae | Ile + 4-nitroaniline | - |
? | |
L-Pro-4-nitroanilide + H2O | - |
Aspergillus oryzae | L-Pro + 4-nitroaniline | - |
? | |
Leu-4-nitroanilide + H2O | best Xaa-4-nitroanilide substrate | Aspergillus oryzae | Leu + 4-nitroaniline | - |
? | |
Leu-Ala-Pro-Tyr-Lys-amide + H2O | - |
Aspergillus oryzae | Leu + Ala-Pro-Tyr-Lys-amide | - |
? | |
Lys-4-nitroanilide + H2O | - |
Aspergillus oryzae | Lys + 4-nitroaniline | - |
? | |
additional information | non-specific enzyme, broad substrate spectrum, but no hydrolysis of Xaa-Pro bonds | Aspergillus oryzae | ? | - |
? | |
Pro-Ala-Pro-Tyr-Lys-amide + H2O | best pentapeptide substrate | Aspergillus oryzae | Pro + Ala-Pro-Tyr-Lys-amide | - |
? | |
Val-4-nitroanilide + H2O | - |
Aspergillus oryzae | Val + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56000, deglycosylated recombinant enzyme, SDS-PAGE, x * 51950, mature enzyme, amino acid sequence calculation | Aspergillus oryzae |
Synonyms | Comment | Organism |
---|---|---|
aminopeptidase II | - |
Aspergillus oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Aspergillus oryzae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
20 min, 65% residual activity, compared to the activity at 37°C | Aspergillus oryzae |
75 | - |
20 min, 46% residual activity, compared to the activity at 37°C | Aspergillus oryzae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
Pro-Ala-Pro-Tyr-Lys-amide | pH 7.5, 21°C, recombinant enzyme | Aspergillus oryzae | |
0.08 | - |
L-Pro-4-nitroanilide | pH 7.5, 21°C, recombinant enzyme | Aspergillus oryzae | |
0.1 | - |
Val-4-nitroanilide | pH 7.5, 21°C, recombinant enzyme | Aspergillus oryzae | |
0.13 | - |
Glu-4-nitroanilide | pH 7.5, 21°C, recombinant enzyme | Aspergillus oryzae | |
0.18 | - |
Ile-4-nitroanilide | pH 7.5, 21°C, recombinant enzyme | Aspergillus oryzae | |
0.68 | - |
Ala-4-nitroanilide | pH 7.5, 21°C, recombinant enzyme | Aspergillus oryzae | |
0.72 | - |
Glu-Ala-Pro-Tyr-Lys-amide | pH 7.5, 21°C, recombinant enzyme | Aspergillus oryzae | |
0.88 | - |
Gly-Ala-Pro-Tyr-Lys-amide | pH 7.5, 21°C, recombinant enzyme | Aspergillus oryzae | |
1.8 | - |
Lys-4-nitroanilide | pH 7.5, 21°C, recombinant enzyme | Aspergillus oryzae | |
3.13 | - |
Ala-Ala-Pro-Tyr-Lys-amide | pH 7.5, 21°C, recombinant enzyme | Aspergillus oryzae | |
3.13 | - |
Leu-Ala-Pro-Tyr-Lys-amide | pH 7.5, 21°C, recombinant enzyme | Aspergillus oryzae | |
3.5 | - |
Leu-4-nitroanilide | pH 7.5, 21°C, recombinant enzyme | Aspergillus oryzae | |
3.67 | - |
Leu-Ala-Pro-Tyr-Lys-amide | pH 7.5, 21°C, recombinant enzyme | Aspergillus oryzae | |
4.34 | - |
Ala-Ala-Pro-Tyr-Lys-amide | pH 7.5, 21°C, recombinant enzyme | Aspergillus oryzae | |
6.08 | - |
Ala-4-nitroanilide | pH 7.5, 21°C, recombinant enzyme | Aspergillus oryzae | |
6.08 | - |
Gly-Ala-Pro-Tyr-Lys-amide | pH 7.5, 21°C, recombinant enzyme | Aspergillus oryzae | |
6.08 | - |
Glu-Ala-Pro-Tyr-Lys-amide | pH 7.5, 21°C, recombinant enzyme | Aspergillus oryzae | |
6.17 | - |
Pro-Ala-Pro-Tyr-Lys-amide | pH 7.5, 21°C, recombinant enzyme | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Aspergillus oryzae |