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Literature summary for 3.4.11.22 extracted from

  • Blinkovsky, A.M.; Byun, T.; Brown, K.M.; Golightly, E.J.; Klotz, A.V.
    A non-specific aminopeptidase from Aspergillus (2000), Biochim. Biophys. Acta, 1480, 171-181.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, functional overexpression in Fusarium venenatum and Aspergillus oryzae Aspergillus oryzae

Inhibitors

Inhibitors Comment Organism Structure
EDTA 10% inhibition at 1 mM Aspergillus oryzae
o-phenanthroline 91% inhibition at 1 mM Aspergillus oryzae
PMSF 5% inhibition at 1 mM Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5
-
Glu-4-nitroanilide pH 7.5, 21°C, recombinant enzyme Aspergillus oryzae
3.5
-
Ala-4-nitroanilide pH 7.5, 21°C, recombinant enzyme Aspergillus oryzae
7
-
Leu-4-nitroanilide pH 7.5, 21°C, recombinant enzyme Aspergillus oryzae
9
-
Lys-4-nitroanilide pH 7.5, 21°C, recombinant enzyme Aspergillus oryzae
13
-
Val-4-nitroanilide pH 7.5, 21°C, recombinant enzyme Aspergillus oryzae
15
-
L-Pro-4-nitroanilide pH 7.5, 21°C, recombinant enzyme Aspergillus oryzae
51
-
Ile-4-nitroanilide pH 7.5, 21°C, recombinant enzyme Aspergillus oryzae

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secretion to the culture medium Aspergillus oryzae
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required, metalloenzyme, bound at the active site, enzyme contains the HEXXH motif Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51950
-
x * 56000, deglycosylated recombinant enzyme, SDS-PAGE, x * 51950, mature enzyme, amino acid sequence calculation Aspergillus oryzae
56000
-
x * 56000, deglycosylated recombinant enzyme, SDS-PAGE, x * 51950, mature enzyme, amino acid sequence calculation Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
strain ATCC 20386
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N-glycosylation Aspergillus oryzae

Purification (Commentary)

Purification (Comment) Organism
from culture supernatant Aspergillus oryzae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
reaction velocity in descending order with Xaa being Pro, Ala, Leu, Gly, and Glu Aspergillus oryzae
4.12
-
with Leu-4-nitroanilide as substrate, measurement of absorption Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-4-nitroanilide + H2O
-
Aspergillus oryzae Ala + 4-nitroaniline
-
?
Ala-Ala-Pro-Tyr-Lys-amide + H2O
-
Aspergillus oryzae Ala + Ala-Pro-Tyr-Lys-amide
-
?
Glu-4-nitroanilide + H2O
-
Aspergillus oryzae Glu + 4-nitroaniline
-
?
Glu-Ala-Pro-Tyr-Lys-amide + H2O
-
Aspergillus oryzae Glu + Ala-Pro-Tyr-Lys-amide
-
?
Gly-Ala-Pro-Tyr-Lys-amide + H2O
-
Aspergillus oryzae Gly + Ala-Pro-Tyr-Lys-amide
-
?
Ile-4-nitroanilide + H2O
-
Aspergillus oryzae Ile + 4-nitroaniline
-
?
L-Pro-4-nitroanilide + H2O
-
Aspergillus oryzae L-Pro + 4-nitroaniline
-
?
Leu-4-nitroanilide + H2O best Xaa-4-nitroanilide substrate Aspergillus oryzae Leu + 4-nitroaniline
-
?
Leu-Ala-Pro-Tyr-Lys-amide + H2O
-
Aspergillus oryzae Leu + Ala-Pro-Tyr-Lys-amide
-
?
Lys-4-nitroanilide + H2O
-
Aspergillus oryzae Lys + 4-nitroaniline
-
?
additional information non-specific enzyme, broad substrate spectrum, but no hydrolysis of Xaa-Pro bonds Aspergillus oryzae ?
-
?
Pro-Ala-Pro-Tyr-Lys-amide + H2O best pentapeptide substrate Aspergillus oryzae Pro + Ala-Pro-Tyr-Lys-amide
-
?
Val-4-nitroanilide + H2O
-
Aspergillus oryzae Val + 4-nitroaniline
-
?

Subunits

Subunits Comment Organism
? x * 56000, deglycosylated recombinant enzyme, SDS-PAGE, x * 51950, mature enzyme, amino acid sequence calculation Aspergillus oryzae

Synonyms

Synonyms Comment Organism
aminopeptidase II
-
Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Aspergillus oryzae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
20 min, 65% residual activity, compared to the activity at 37°C Aspergillus oryzae
75
-
20 min, 46% residual activity, compared to the activity at 37°C Aspergillus oryzae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
Pro-Ala-Pro-Tyr-Lys-amide pH 7.5, 21°C, recombinant enzyme Aspergillus oryzae
0.08
-
L-Pro-4-nitroanilide pH 7.5, 21°C, recombinant enzyme Aspergillus oryzae
0.1
-
Val-4-nitroanilide pH 7.5, 21°C, recombinant enzyme Aspergillus oryzae
0.13
-
Glu-4-nitroanilide pH 7.5, 21°C, recombinant enzyme Aspergillus oryzae
0.18
-
Ile-4-nitroanilide pH 7.5, 21°C, recombinant enzyme Aspergillus oryzae
0.68
-
Ala-4-nitroanilide pH 7.5, 21°C, recombinant enzyme Aspergillus oryzae
0.72
-
Glu-Ala-Pro-Tyr-Lys-amide pH 7.5, 21°C, recombinant enzyme Aspergillus oryzae
0.88
-
Gly-Ala-Pro-Tyr-Lys-amide pH 7.5, 21°C, recombinant enzyme Aspergillus oryzae
1.8
-
Lys-4-nitroanilide pH 7.5, 21°C, recombinant enzyme Aspergillus oryzae
3.13
-
Ala-Ala-Pro-Tyr-Lys-amide pH 7.5, 21°C, recombinant enzyme Aspergillus oryzae
3.13
-
Leu-Ala-Pro-Tyr-Lys-amide pH 7.5, 21°C, recombinant enzyme Aspergillus oryzae
3.5
-
Leu-4-nitroanilide pH 7.5, 21°C, recombinant enzyme Aspergillus oryzae
3.67
-
Leu-Ala-Pro-Tyr-Lys-amide pH 7.5, 21°C, recombinant enzyme Aspergillus oryzae
4.34
-
Ala-Ala-Pro-Tyr-Lys-amide pH 7.5, 21°C, recombinant enzyme Aspergillus oryzae
6.08
-
Ala-4-nitroanilide pH 7.5, 21°C, recombinant enzyme Aspergillus oryzae
6.08
-
Gly-Ala-Pro-Tyr-Lys-amide pH 7.5, 21°C, recombinant enzyme Aspergillus oryzae
6.08
-
Glu-Ala-Pro-Tyr-Lys-amide pH 7.5, 21°C, recombinant enzyme Aspergillus oryzae
6.17
-
Pro-Ala-Pro-Tyr-Lys-amide pH 7.5, 21°C, recombinant enzyme Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
Aspergillus oryzae