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Literature summary for 3.4.11.22 extracted from

  • Bienvenue, D.L.; Gilner, D.; Holz, R.C.
    Hydrolysis of thionopeptides by the aminopeptidase from Aeromonas proteolytica: insight into substrate binding (2002), Biochemistry, 41, 3712-3719.
    View publication on PubMed
Search for more literature for 3.4.11.22

Inhibitors

Inhibitors Comment Organism Structure
1-Phenyl-2-thiourea competitive Vibrio proteolyticus
Urea competitive Vibrio proteolyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4
-
 thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with 2 Cd2+ bound Vibrio proteolyticus
4
-
 thionoleucine-S-anilide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
4
-
 thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
4.6
-
 L-leucine 4-nitroanilide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
5
-
 thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
7
-
 thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
7.8
-
 L-leucine 4-nitroanilide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
8
-
 thionoleucine-S-anilide pH 8.0, 25°C, enzyme with 2 Cd2+ bound Vibrio proteolyticus
10
-
 thionoleucine-S-anilide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
11
-
 thionoleucine-S-anilide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
15
-
 L-leucine 4-nitroanilide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
32
-
 L-leucine-4-anisidide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
34
-
 L-leucine-4-anisidide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
45
-
 L-leucin-anilide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
50
-
 L-leucine-4-anisidide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
61
-
 L-leucine-anilide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
78
-
 L-leucine-anilide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ the bimetallic enzyme contains eihter 2 Zn2+ or 2 Cd2+ or Zn2+ and Cd2+ Vibrio proteolyticus
Co2+ Co2+ can substitute for Cd2+ in the bimetallic enzyme Vibrio proteolyticus
Zn2+ the bimetallic enzyme contains eihter 2 Zn2+ or 2 Cd2+ or Zn2+ and Cd2+ Vibrio proteolyticus

Organism

Organism UniProt Comment Textmining
Vibrio proteolyticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Vibrio proteolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-leucine 4-nitroanilide + H2O
-
 Vibrio proteolyticus L-leucine + 4-nitroaniline
-
 ?
L-leucine-4-anisidide + H2O
-
 Vibrio proteolyticus L-leucine + anisidine
-
 ?
L-leucine-anilide + H2O
-
 Vibrio proteolyticus L-leucine + aniline
-
 ?
thionoleucine-4-anisidide + H2O
-
 Vibrio proteolyticus thionoleucine + anisidine
-
 ?
thionoleucine-S-anilide + H2O
-
 Vibrio proteolyticus thionoleucine + aniline
-
 ?

Synonyms

Synonyms Comment Organism
AAP
-
 Vibrio proteolyticus
Aeromonas proteolytica aminopeptidase
-
 Vibrio proteolyticus
More see also EC 3.4.11.10 Vibrio proteolyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Vibrio proteolyticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3
-
 L-leucine 4-nitroanilide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
3.8
-
 L-leucine-4-anisidide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
5.3
-
 L-leucine 4-nitroanilide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
6.3
-
 L-leucine-anilide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
7.5
-
 thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
7.7
-
 thionoleucine-S-anilide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
7.9
-
 thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with 2 Cd2+ bound Vibrio proteolyticus
9.3
-
 L-leucine-4-anisidide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
9.5
-
 L-leucine-4-anisidide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
10.6
-
 thionoleucine-S-anilide pH 8.0, 25°C, enzyme with 2 Cd2+ bound Vibrio proteolyticus
11.1
-
 thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
11.5
-
 thionoleucine-S-anilide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
12.2
-
 thionoleucine-4-anisidide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
15.1
-
 L-leucine-anilide pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound Vibrio proteolyticus
16
-
 L-leucine-anilide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus
16.9
-
 thionoleucine-S-anilide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
23.3
-
 thionoleucine-S-anilide pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound Vibrio proteolyticus
60
-
 L-leucine 4-nitroanilide pH 8.0, 25°C, enzyme with 2 Zn2+ bound Vibrio proteolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Vibrio proteolyticus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.24
-
 1-Phenyl-2-thiourea pH 8.0, 25°C Vibrio proteolyticus
4.6
-
 Urea pH 8.0, 25°C Vibrio proteolyticus