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Literature summary for 3.4.11.22 extracted from

  • Moriyasu, Y.; Sakano, K.; Tazawa, M.
    Vacuolar/extravacuolar distribution of aminopeptidase in giant alga Chara australis and partial purification of one such enzyme (1987), Plant Physiol., 84, 720-725.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
1,10 phenanthroline
-
Chara australis
EDTA
-
Chara australis
iodoacetate
-
Chara australis
additional information no inhibition by diisopropyl fluorophosphate, phenylmethylsulfonyl fluoride Chara australis
N-tosyl-L-Phe chloromethyl ketone slightly inhibitory Chara australis
p-chloromercuribenzoate
-
Chara australis

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information extravacuolar, , 60% of enzyme activity Chara australis
-
-
vacuole 40% of enzyme activity Chara australis 5773
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
x * 62000 + x * 44000, SDS-PAGE Chara australis
62000
-
x * 62000 + x * 44000, SDS-PAGE Chara australis
120000
-
gel permeation chromatography Chara australis

Organism

Organism UniProt Comment Textmining
Chara australis
-
giant alga
-

Purification (Commentary)

Purification (Comment) Organism
partially Chara australis

Source Tissue

Source Tissue Comment Organism Textmining
whole plant
-
Chara australis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.36
-
-
Chara australis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala 2-naphthylamide + H2O
-
Chara australis Ala + 2-naphthylamine
-
?
Arg 2-naphthylamide + H2O
-
Chara australis Arg + 2-naphthylamine
-
?
Asp 2-naphthylamide + H2O very slight activity Chara australis Asp + 2-naphthylamine
-
?
Leu 2-naphthylamide + H2O
-
Chara australis Leu + 2-naphthylamine
-
?
Lys 2-naphthylamide + H2O
-
Chara australis Lys + 2-naphthylamine
-
?
additional information no hydrolyis of N-alpha-benzoyl-DL-Arg 2-naphthylamide Chara australis ?
-
?
Phe 2-naphthylamide + H2O
-
Chara australis Phe + 2-naphthylamine
-
?
Pro 2-naphthylamide + H2O
-
Chara australis Pro + 2-naphthylamine
-
?
Ser 2-naphthylamide + H2O
-
Chara australis Ser + 2-naphthylamine
-
?
Tyr 2-naphthylamide + H2O
-
Chara australis Tyr + 2-naphthylamine
-
?
Val 2-naphthylamide + H2O slight activity Chara australis Val + 2-naphthylamine
-
?

Subunits

Subunits Comment Organism
? x * 62000 + x * 44000, SDS-PAGE Chara australis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
substrate Leu 2-naphthylamide Chara australis
7 8 substrate Ala 2-naphthylamide Chara australis