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Literature summary for 3.4.11.22 extracted from
- Vacuolar/extravacuolar distribution of aminopeptidase in giant alga Chara australis and partial purification of one such enzyme (1987), Plant Physiol., 84, 720-725.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10 phenanthroline | - |
Chara australis |  |
| EDTA | - |
Chara australis | |
| iodoacetate | - |
Chara australis | |
| additional information | no inhibition by diisopropyl fluorophosphate, phenylmethylsulfonyl fluoride | Chara australis | |
| N-tosyl-L-Phe chloromethyl ketone | slightly inhibitory | Chara australis | |
| p-chloromercuribenzoate | - |
Chara australis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | extravacuolar, , 60% of enzyme activity | Chara australis | - |
- |
| vacuole | 40% of enzyme activity | Chara australis | 5773 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
x * 62000 + x * 44000, SDS-PAGE | Chara australis |
| 62000 | - |
x * 62000 + x * 44000, SDS-PAGE | Chara australis |
| 120000 | - |
gel permeation chromatography | Chara australis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chara australis | - |
giant alga | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially | Chara australis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| whole plant | - |
Chara australis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.36 | - |
- |
Chara australis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ala 2-naphthylamide + H2O | - |
Chara australis | Ala + 2-naphthylamine | - |
? | |
| Arg 2-naphthylamide + H2O | - |
Chara australis | Arg + 2-naphthylamine | - |
? | |
| Asp 2-naphthylamide + H2O | very slight activity | Chara australis | Asp + 2-naphthylamine | - |
? | |
| Leu 2-naphthylamide + H2O | - |
Chara australis | Leu + 2-naphthylamine | - |
? | |
| Lys 2-naphthylamide + H2O | - |
Chara australis | Lys + 2-naphthylamine | - |
? | |
| additional information | no hydrolyis of N-alpha-benzoyl-DL-Arg 2-naphthylamide | Chara australis | ? | - |
? | |
| Phe 2-naphthylamide + H2O | - |
Chara australis | Phe + 2-naphthylamine | - |
? | |
| Pro 2-naphthylamide + H2O | - |
Chara australis | Pro + 2-naphthylamine | - |
? | |
| Ser 2-naphthylamide + H2O | - |
Chara australis | Ser + 2-naphthylamine | - |
? | |
| Tyr 2-naphthylamide + H2O | - |
Chara australis | Tyr + 2-naphthylamine | - |
? | |
| Val 2-naphthylamide + H2O | slight activity | Chara australis | Val + 2-naphthylamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 62000 + x * 44000, SDS-PAGE | Chara australis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
substrate Leu 2-naphthylamide | Chara australis |
| 7 | 8 | substrate Ala 2-naphthylamide | Chara australis |
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