Activating Compound | Comment | Organism | Structure |
---|---|---|---|
E64 | slight activation | Lactobacillus delbrueckii subsp. lactis |
Cloned (Comment) | Organism |
---|---|
gene pepA, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Lactococcus lactis ssp. lactis |
gene pepA, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Lactobacillus delbrueckii subsp. lactis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 21% at 1.0 mM, complete inhibition at 10 mM | Lactobacillus delbrueckii subsp. lactis | |
1,10-phenanthroline | complete inhibition at 1-10 mM | Lactococcus lactis ssp. lactis | |
2-mercaptoethanol | 58% inhibition at 0.01 mM, 96% at 0.1 mM | Lactococcus lactis ssp. lactis | |
acetone | 21.5% inhibition at 4.2% v/v | Lactobacillus delbrueckii subsp. lactis | |
acetone | 37.2% inhibition at 4.2% v/v | Lactococcus lactis ssp. lactis | |
dimethylformamide | 78.1% inhibition at 4.2% v/v | Lactobacillus delbrueckii subsp. lactis | |
dimethylformamide | 84.9% inhibition at 4.2% v/v | Lactococcus lactis ssp. lactis | |
DMSO | 15.1% inhibition at 4.2% v/v | Lactobacillus delbrueckii subsp. lactis | |
DMSO | 13.9% inhibition at 4.2% v/v | Lactococcus lactis ssp. lactis | |
DTT | 12% inhibition at 0.1 mM | Lactobacillus delbrueckii subsp. lactis | |
DTT | 69% inhibition at 0.001 mM, 94% at 0.1 mM | Lactococcus lactis ssp. lactis | |
E64 | slight inhibition | Lactococcus lactis ssp. lactis | |
EDTA | complete inhibition at 1.0 mM | Lactobacillus delbrueckii subsp. lactis | |
EDTA | complete inhibition at 1.0 mM | Lactococcus lactis ssp. lactis | |
ethanol | 16.3% inhibition at 4.2% v/v | Lactobacillus delbrueckii subsp. lactis | |
ethanol | 25.7% inhibition at 4.2% v/v | Lactococcus lactis ssp. lactis | |
Imidazol | 34% inhibition at 40 mM | Lactobacillus delbrueckii subsp. lactis | |
Imidazol | 21% inhibition at 40 mM | Lactococcus lactis ssp. lactis | |
L-Asp | 39.5% inhibition at 10 mM, no inhibition at 0.1-1.0 mM, product inhibition versus substrate L-Asp-4-nitroanilide, and 33.8% inhibition at 10 mM and no inhibition at 0.1-1.0 mM, product inhibition versus substrate L-Glu-4-nitroanilide | Lactobacillus delbrueckii subsp. lactis | |
L-Asp | 21.8% inhibition at 10 mM and no inhibition at 0.1-1.0 mM, product inhibition versus substrate L-Asp-4-nitroanilide, and 24.0% inhibition at 10 mM and no inhibition at 0.1-1.0 mM, product inhibition versus substrate L-Glu-4-nitroanilide | Lactococcus lactis ssp. lactis | |
L-Glu | product inhibition of enzyme Lc-PepA, 8.4% inhibition at 0.1 mM, 17.7% at 10 mM, product inhibition versus substrate L-Asp-4-nitroanilide, and 1.47% inhibition at 0.1 mM, 31.4% at 10 mM, product inhibition versus substrate L-Glu-4-nitroanilide | Lactococcus lactis ssp. lactis | |
additional information | enzyme Lb-PepA is not product-inhibited by L-Glu. Lb-PepA activity decreases significantly with metal salt concentrations above the optimum concentrations determined. Poor inhibition at 1.0 mM 2-mercaptoethanol, no inhibition by pepstatin A at 0.1-10 mM | Lactobacillus delbrueckii subsp. lactis | |
pepstatin A | slight inhibition | Lactococcus lactis ssp. lactis | |
PMSF | 35% inhibition at 10 mM | Lactobacillus delbrueckii subsp. lactis | |
PMSF | 32% inhibition at 10 mM | Lactococcus lactis ssp. lactis | |
SDS | 73% at 1.0 mM, complete inhibition at 10 mM | Lactobacillus delbrueckii subsp. lactis | |
SDS | 63% at 1.0 mM, 97% inhibition at 10 mM | Lactococcus lactis ssp. lactis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Lactococcus lactis ssp. lactis | |
additional information | - |
additional information | Michaelis-Menten kinetics | Lactobacillus delbrueckii subsp. lactis | |
0.19 | - |
L-Glu-4-nitroanilide | pH 6.0, 60°C, recombinant enzyme | Lactococcus lactis ssp. lactis | |
0.34 | - |
L-Asp-4-nitroanilide | pH 6.0, 60°C, recombinant enzyme | Lactococcus lactis ssp. lactis | |
1.21 | - |
L-Asp-4-nitroanilide | pH 6.0, 60°C, recombinant enzyme | Lactobacillus delbrueckii subsp. lactis | |
25.2 | - |
L-Glu-4-nitroanilide | pH 6.0, 60°C, recombinant enzyme | Lactobacillus delbrueckii subsp. lactis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates, best at 0.3125 mM | Lactococcus lactis ssp. lactis | |
Co2+ | activates, best at 0.625 mM | Lactobacillus delbrueckii subsp. lactis | |
Mn2+ | activates, best at 0.3125 mM | Lactococcus lactis ssp. lactis | |
Mn2+ | activates, best at 1.25 mM | Lactobacillus delbrueckii subsp. lactis | |
additional information | Lb-PepA activity decreases significantly with metal salt concentrations above the optimum concentrations determined | Lactobacillus delbrueckii subsp. lactis | |
additional information | Lc-PepA activity decreases only slightly or is almost constant with metal salt concentrations above the optimum concentrations determined | Lactococcus lactis ssp. lactis | |
Zn2+ | activates, best at 5 mM | Lactococcus lactis ssp. lactis | |
Zn2+ | slight activation, best at 0.625 mM | Lactobacillus delbrueckii subsp. lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
470000 | - |
recombinant His6-tagged enzyme, gel filtration | Lactococcus lactis ssp. lactis |
508000 | - |
recombinant His6-tagged enzyme, gel filtration | Lactobacillus delbrueckii subsp. lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus delbrueckii subsp. lactis | F0HXE4 | - |
- |
Lactobacillus delbrueckii subsp. lactis DSM 20072 | F0HXE4 | - |
- |
Lactococcus lactis ssp. lactis | - |
- |
- |
Lactococcus lactis ssp. lactis DSM 20481 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Lactococcus lactis ssp. lactis |
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Lactobacillus delbrueckii subsp. lactis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the Lb-PepA activity decreases during the stationary growth phase, which is caused by degradation of the enzyme | Lactobacillus delbrueckii subsp. lactis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Asp-4-nitroanilide + H2O | - |
Lactococcus lactis ssp. lactis | L-Asp + 4-nitroaniline | - |
? | |
L-Asp-4-nitroanilide + H2O | - |
Lactobacillus delbrueckii subsp. lactis | L-Asp + 4-nitroaniline | - |
? | |
L-Asp-4-nitroanilide + H2O | - |
Lactobacillus delbrueckii subsp. lactis DSM 20072 | L-Asp + 4-nitroaniline | - |
? | |
L-Asp-4-nitroanilide + H2O | - |
Lactococcus lactis ssp. lactis DSM 20481 | L-Asp + 4-nitroaniline | - |
? | |
L-Glu-4-nitroanilide + H2O | - |
Lactococcus lactis ssp. lactis | L-Glu + 4-nitroaniline | - |
? | |
L-Glu-4-nitroanilide + H2O | - |
Lactobacillus delbrueckii subsp. lactis | L-Glu + 4-nitroaniline | - |
? | |
L-Glu-4-nitroanilide + H2O | - |
Lactobacillus delbrueckii subsp. lactis DSM 20072 | L-Glu + 4-nitroaniline | - |
? | |
L-Glu-4-nitroanilide + H2O | - |
Lactococcus lactis ssp. lactis DSM 20481 | L-Glu + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
homododecamer | 12 * 40000, recombinant His6-tagged enzyme, SDS-PAGE, 12 * 39400, about, sequence calculation | Lactococcus lactis ssp. lactis |
homododecamer | 12 * 41000, recombinant His6-tagged enzyme, SDS-PAGE, 12 * 41100, about, sequence calculation | Lactobacillus delbrueckii subsp. lactis |
Synonyms | Comment | Organism |
---|---|---|
glutamyl (aspartyl)-specific aminopeptidase A | - |
Lactococcus lactis ssp. lactis |
glutamyl (aspartyl)-specific aminopeptidase A | - |
Lactobacillus delbrueckii subsp. lactis |
Lb-PepA | - |
Lactobacillus delbrueckii subsp. lactis |
Lc-PepA | - |
Lactococcus lactis ssp. lactis |
More | see also EC 3.4.11.7 | Lactococcus lactis ssp. lactis |
More | see also EC 3.4.11.7 | Lactobacillus delbrueckii subsp. lactis |
PepA | - |
Lactococcus lactis ssp. lactis |
PepA | - |
Lactobacillus delbrueckii subsp. lactis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Lactobacillus delbrueckii subsp. lactis |
65 | - |
- |
Lactococcus lactis ssp. lactis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 80 | recombinant enzyme, activity range, profile overview | Lactococcus lactis ssp. lactis |
20 | 75 | recombinant enzyme, activity range, profile overview | Lactobacillus delbrueckii subsp. lactis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
purified recombinant His-tagged enzyme, completely stable for over 70 h | Lactococcus lactis ssp. lactis |
- |
- |
purified recombinant His-tagged enzyme, over 80% activity remaining after 70 h | Lactobacillus delbrueckii subsp. lactis |
40 | - |
purified recombinant His-tagged enzyme, over 60% activity remaining after 70 h | Lactobacillus delbrueckii subsp. lactis |
45 | - |
purified recombinant His-tagged enzyme, half-life at 5°C is about 20 h, inactivation within 70 h | Lactococcus lactis ssp. lactis |
50 | - |
purified recombinant His-tagged enzyme, over 15% activity remaining after 70 h | Lactobacillus delbrueckii subsp. lactis |
55 | - |
purified recombinant His-tagged enzyme, half-life at 65°C is about 4-5 h, inactivation within 65 h | Lactococcus lactis ssp. lactis |
60 | - |
purified recombinant His-tagged enzyme, inactivation within 10 h | Lactobacillus delbrueckii subsp. lactis |
65 | - |
purified recombinant His-tagged enzyme, half-life at 65°C is about 2 h, inactivation within 10 h | Lactococcus lactis ssp. lactis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Lactobacillus delbrueckii subsp. lactis |
8 | - |
- |
Lactococcus lactis ssp. lactis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | recombinant enzyme, activity range, profile overview | Lactococcus lactis ssp. lactis |
5 | 8 | recombinant enzyme, activity range, profile overview | Lactobacillus delbrueckii subsp. lactis |