Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.11.21 extracted from

  • Teuscher, F.; Lowther, J.; Skinner-Adams, T.S.; Spielmann, T.; Dixon, M.W.; Stack, C.M.; Donnelly, S.; Mucha, A.; Kafarski, P.; Vassiliou, S.; Gardiner, D.L.; Dalton, J.P.; Trenholme, K.R.
    The M18 aspartyl aminopeptidase of the human malaria parasite Plasmodium falciparum (2007), J. Biol. Chem., 282, 30817-30826.
    View publication on PubMed
Search for more literature for 3.4.11.21

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf9 insect cells Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
1-amino-1,3-propanediphosphonic acid
-
 Plasmodium falciparum
3-amino-3-(P-methylphosphinyl)propionic acid
-
 Plasmodium falciparum
3-amino-3-phosphonopropionic acid
-
 Plasmodium falciparum
3-amino-3-[P-(2-carboxypropyl)phosphinyl]propionic acid
-
 Plasmodium falciparum
4-amino-4-(P-phenylphosphinyl)butyric acid 20% inhibition at 1.83 mM Plasmodium falciparum
4-amino-4-phosphonobutyric acid
-
 Plasmodium falciparum
4-amino-4-[P-(2-carboxypropyl)phosphinyl]butyric acid
-
 Plasmodium falciparum
5-amino-5-phosphonopentanoic acid
-
 Plasmodium falciparum
EDTA activity is reduced to 14% after incubating with 10 mM EDTA Plasmodium falciparum
o-phenanthroline activity is reduced to 8.3% after incubating with 20 mM o-phenanthroline Plasmodium falciparum
Zn2+ Zn2+ abolishes activity at 1 mM Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.135
-
 Glu-7-amido-4-methylcoumarin
-
 Plasmodium falciparum
0.136
-
 Glu-7-amido-4-methylcoumarin in the presence of 1 mM Co2+ Plasmodium falciparum
0.327
-
 Asp-7-amido-4-methylcoumarin in the presence of 1 mM Co2+ Plasmodium falciparum
0.385
-
 Asp-7-amido-4-methylcoumarin
-
 Plasmodium falciparum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Plasmodium falciparum 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ enhanced activity Plasmodium falciparum
additional information activity is not enhanced by Ca2+, Fe2+, Mg2+, Mn2+, and Ni2+ Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 8 * 65000, SDS-PAGE Plasmodium falciparum
70000
-
 8 * 70000, high performance liquid chromatography Plasmodium falciparum
520000
-
 SDS-PAGE Plasmodium falciparum
560000
-
 high performance liquid chromatography Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA-agarose column chromatography and Superdex-200 gel filtration Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Asp-7-amido-4-methylcoumarin + H2O
-
 Plasmodium falciparum Asp + 7-amino-4-methylcoumarin
-
 ?
Glu-7-amido-4-methylcoumarin + H2O
-
 Plasmodium falciparum Glu + 7-amino-4-methylcoumarin
-
 ?
additional information no hydrolysis is observed against Leu-7-amido-4-methylcoumarin, Phe-7-amido-4-methylcoumarin, Ala-7-amido-4-methylcoumarin, Pro-7-amido-4-methylcoumarin, Gly-7-amido-4-methylcoumarin, Val-7-amido-4-methylcoumarin, Arg-7-amido-4-methylcoumarin, and Ile-7-amido-4-methylcoumarin Plasmodium falciparum ?
-
 ?

Subunits

Subunits Comment Organism
homooctamer 8 * 65000, SDS-PAGE Plasmodium falciparum
homooctamer 8 * 70000, high performance liquid chromatography Plasmodium falciparum

Synonyms

Synonyms Comment Organism
M18 aspartyl aminopeptidase
-
 Plasmodium falciparum
M18AAP
-
 Plasmodium falciparum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 stable when stored for 1 h at 37°C over the pH range 6 to 11 Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.011
-
 Glu-7-amido-4-methylcoumarin at pH 7.5, in the absesence of Co2+ Plasmodium falciparum
0.05
-
 Asp-7-amido-4-methylcoumarin at pH 7.5, in the absence of Co2+ Plasmodium falciparum
0.33
-
 Glu-7-amido-4-methylcoumarin at pH 7.5, in the presence of 1 mM Co2+ Plasmodium falciparum
0.354
-
 Asp-7-amido-4-methylcoumarin at pH 7.5, in the presence of 1 mM Co2+ Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Plasmodium falciparum

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
 Plasmodium falciparum

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 11 stable when stored for 1 h at 37°C over the pH range 6 to 11 Plasmodium falciparum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00034
-
 4-amino-4-phosphonobutyric acid at pH 7.5 Plasmodium falciparum
0.0044
-
 1-amino-1,3-propanediphosphonic acid at pH 7.5 Plasmodium falciparum
0.0271
-
 3-amino-3-phosphonopropionic acid at pH 7.5 Plasmodium falciparum
0.0427
-
 4-amino-4-[P-(2-carboxypropyl)phosphinyl]butyric acid at pH 7.5 Plasmodium falciparum
0.105
-
 5-amino-5-phosphonopentanoic acid at pH 7.5 Plasmodium falciparum
0.183
-
 3-amino-3-[P-(2-carboxypropyl)phosphinyl]propionic acid at pH 7.5 Plasmodium falciparum
0.395
-
 3-amino-3-(P-methylphosphinyl)propionic acid at pH 7.5 Plasmodium falciparum