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Literature summary for 3.4.11.20 extracted from

  • Gonzalez-Bacerio, J.; Osuna, J.; Ponce, A.; Fando, R.; Figarella, K.; Mendez, Y.; Charli, J.L.; Chavez, M.D.
    High-level expression in Escherichia coli, purification and kinetic characterization of Plasmodium falciparum M1-aminopeptidase (2014), Protein Expr. Purif., 104C, 103-114.
    View publication on PubMed
Search for more literature for 3.4.11.20

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
additional information synthesis and cloning of an optimized gene, with a codon adaptation index and an average G/C content higher than the native gene. Optimal expression is achieved by induction with 1 mM IPTG at 37°C for 18 h. This allows obtaining 100 mg of recombinant enzyme per liter of culture medium. The protein shows only limited signs of proteolytic degradation, and purification in a single step by immobilized metal ion affinity chromatography increases purity 27fold Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 1 mM, 80% inhibition Plasmodium falciparum
Zn2+ 0.1 mM, 80% inhibition Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.248
-
 L-Lys 4-nitroanilide pH 7.2, 37°C Plasmodium falciparum
0.27
-
 L-Arg 4-nitroanilide pH 7.2, 37°C Plasmodium falciparum
0.304
-
 L-Ala 4-nitroanilide pH 7.2, 37°C Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
106130
-
 x * 106130, calculated for recombinant protein including His-tag Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum Q8IEK1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala 4-nitroanilide + H2O
-
 Plasmodium falciparum L-Ala + 4-nitroaniline
-
 ?
L-Arg 4-nitroanilide + H2O
-
 Plasmodium falciparum L-Arg + 4-nitroaniline
-
 ?
L-Leu 4-nitroanilide + H2O
-
 Plasmodium falciparum L-Leu + 4-nitroaniline
-
 ?
L-Lys 4-nitroanilide + H2O
-
 Plasmodium falciparum L-Lys + 4-nitroaniline
-
 ?
additional information no substrates: Ile-4-nitroanilide, Val-4-nitroanilide, Gly-4-nitroanilide, Pro-4-nitroanilide Plasmodium falciparum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 106130, calculated for recombinant protein including His-tag Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.15
-
 L-Ala 4-nitroanilide pH 7.2, 37°C Plasmodium falciparum
1.55
-
 L-Lys 4-nitroanilide pH 7.2, 37°C Plasmodium falciparum
1.58
-
 L-Arg 4-nitroanilide pH 7.2, 37°C Plasmodium falciparum
1.65
-
 L-Leu 4-nitroanilide pH 7.2, 37°C Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 7.4
-
 Plasmodium falciparum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.24
-
 L-Ala 4-nitroanilide pH 7.2, 37°C Plasmodium falciparum
5.42
-
 L-Leu 4-nitroanilide pH 7.2, 37°C Plasmodium falciparum
5.84
-
 L-Arg 4-nitroanilide pH 7.2, 37°C Plasmodium falciparum
6.24
-
 L-Lys 4-nitroanilide pH 7.2, 37°C Plasmodium falciparum