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Literature summary for 3.4.11.20 extracted from

  • Ragheb, D.; Dalal, S.; Bompiani, K.M.; Ray, W.K.; Klemba, M.
    Distribution and biochemical properties of an M1-family aminopeptidase in Plasmodium falciparum indicate a role in vacuolar hemoglobin catabolism (2011), J. Biol. Chem., 286, 27255-27265.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
additional information amino acid residue 192-1085 expressed as His-tagged protein in Escherichia coli BL21(DE3) Rosetta2 Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
bestatin
-
Plasmodium falciparum
bestatin methyl ester
-
Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
Met-Phe recombinant protein, pH 7.5, 37┬░C Plasmodium falciparum
0.065
-
Met-Phe recombinant protein, pH 5.5, 37┬░C Plasmodium falciparum
0.086
-
L-arginyl-2-naphthylamide p68/p35 complex, pH 7.5, 37┬░C Plasmodium falciparum
0.11
-
L-arginyl-2-naphthylamide p120, pH 7.5, 37┬░C Plasmodium falciparum
0.13
-
Leu-Leu recombinant protein, pH 7.5, 37┬░C Plasmodium falciparum
0.17
-
L-arginyl-2-naphthylamide recombinant protein, pH 7.5, 37┬░C Plasmodium falciparum
0.21
-
L-alanyl-2-naphthylamide recombinant protein, pH 7.5, 37┬░C Plasmodium falciparum
0.38
-
Met-Phe recombinant protein, pH 5.0, 37┬░C Plasmodium falciparum
0.41
-
Leu-Leu recombinant protein, pH 5.5, 37┬░C Plasmodium falciparum
1.1
-
L-arginyl-2-naphthylamide recombinant protein, pH 5.5, 37┬░C Plasmodium falciparum
1.2
-
Leu-Leu recombinant protein, pH 5.0, 37┬░C Plasmodium falciparum
4.4
-
L-alanyl-2-naphthylamide recombinant protein, pH 5.5, 37┬░C Plasmodium falciparum

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus intraerythroytic parasite Plasmodium falciparum 5634
-
vacuole food vacuole of intraerythroytic parasite Plasmodium falciparum 5773
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
x * 120000, x * complex of 68000 and 35000 (generated by proteolytic cleveage within the loop sequence containing Leu-796 without N-terminal extension (residue 1-194)) Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum Q8IEK1
-
-

Purification (Commentary)

Purification (Comment) Organism
amino acid residue 192-1085 as His-tagged protein: immobilized metal ion affinity chromatography (Ni2+), His-tag removed, gel filtration Plasmodium falciparum
from a modified parasite line expressing an enzyme with a C-terminal hemagglutinin tag: ion exchange chromatography, gel filtration Plasmodium falciparum

Source Tissue

Source Tissue Comment Organism Textmining
trophozoite
-
Plasmodium falciparum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanyl-2-naphthylamide + H2O
-
Plasmodium falciparum L-alanine + 2-naphthylamine
-
?
L-arginyl-2-naphthylamide + H2O
-
Plasmodium falciparum L-arginine + 2-naphthylamine
-
?
Leu-Leu + H2O
-
Plasmodium falciparum 2 L-Leu
-
?
Met-Phe + H2O
-
Plasmodium falciparum L-Met + Phe
-
?

Subunits

Subunits Comment Organism
? x * 120000, x * complex of 68000 and 35000 (generated by proteolytic cleveage within the loop sequence containing Leu-796 without N-terminal extension (residue 1-194)) Plasmodium falciparum

Synonyms

Synonyms Comment Organism
M1-family aminopeptidase
-
Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4
-
Leu-Leu recombinant protein, pH 5.0, 37┬░C Plasmodium falciparum
3 6 L-alanyl-2-naphthylamide recombinant protein, pH 7.5, 37┬░C Plasmodium falciparum
5.9
-
Met-Phe recombinant protein, pH 7.5, 37┬░C Plasmodium falciparum
7.4
-
Met-Phe recombinant protein, pH 5.0, 37┬░C Plasmodium falciparum
7.5
-
Met-Phe recombinant protein, pH 5.5, 37┬░C Plasmodium falciparum
7.9
-
Leu-Leu recombinant protein, pH 5.5, 37┬░C Plasmodium falciparum
9.3
-
L-arginyl-2-naphthylamide p120, pH 7.5, 37┬░C Plasmodium falciparum
9.4
-
L-arginyl-2-naphthylamide recombinant protein, pH 5.5, 37┬░C Plasmodium falciparum
11
-
L-arginyl-2-naphthylamide p68/p35 complex, pH 7.5, 37┬░C Plasmodium falciparum
12
-
Leu-Leu recombinant protein, pH 7.5, 37┬░C Plasmodium falciparum
16
-
L-alanyl-2-naphthylamide recombinant protein, pH 5.5, 37┬░C Plasmodium falciparum
21
-
L-arginyl-2-naphthylamide recombinant protein, pH 7.5, 37┬░C Plasmodium falciparum

pH Range

pH Minimum pH Maximum Comment Organism
5 8.5
-
Plasmodium falciparum

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8.5 at least 90% activity after 60 min Plasmodium falciparum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00012
-
bestatin pH 7.5, 37┬░C Plasmodium falciparum
0.00048
-
bestatin methyl ester pH 7.5, 37┬░C Plasmodium falciparum
0.00048
-
bestatin methyl ester pH 5.5, 37┬░C Plasmodium falciparum
0.0038
-
bestatin pH 5.5, 37┬░C Plasmodium falciparum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2
-
Leu-Leu recombinant protein, pH 5.0, 37┬░C Plasmodium falciparum
3.7
-
L-alanyl-2-naphthylamide recombinant protein, pH 5.5, 37┬░C Plasmodium falciparum
8.7
-
L-arginyl-2-naphthylamide recombinant protein, pH 5.5, 37┬░C Plasmodium falciparum
19
-
Met-Phe recombinant protein, pH 5.0, 37┬░C Plasmodium falciparum
20
-
Leu-Leu recombinant protein, pH 5.5, 37┬░C Plasmodium falciparum
85
-
L-arginyl-2-naphthylamide p120, pH 7.5, 37┬░C Plasmodium falciparum
97
-
Leu-Leu recombinant protein, pH 7.5, 37┬░C Plasmodium falciparum
120
-
Met-Phe recombinant protein, pH 5.5, 37┬░C Plasmodium falciparum
120
-
L-arginyl-2-naphthylamide recombinant protein, pH 7.5, 37┬░C Plasmodium falciparum
130
-
L-arginyl-2-naphthylamide p68/p35 complex, pH 7.5, 37┬░C Plasmodium falciparum
170
-
L-alanyl-2-naphthylamide recombinant protein, pH 7.5, 37┬░C Plasmodium falciparum
270
-
Met-Phe recombinant protein, pH 7.5, 37┬░C Plasmodium falciparum