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Literature summary for 3.4.11.2 extracted from

  • Linz, L.B.; Liu, S.; Chougule, N.P.; Bonning, B.C.
    In vitro evidence supports membrane alanyl aminopeptidase N as a receptor for a plant virus in the pea aphid vector (2015), J. Virol., 89, 11203-11212 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.11.2

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His-tagged enzyme APN in Spodoptera frugiperda SF9 cells using the baculovirus transfection system, recombinant expression of pea aphid APN in Escherichia coli for the production of polyclonal antibodies Acyrthosiphon pisum

Protein Variants

Protein Variants Comment Organism
additional information PEMV virions and a constructed peptide comprised of PEMV CP fused to a proline-rich hinge (-P-) and green fluorescent protein (CP-P-GFP) specifically bind to APN. Recombinant APN expression in Sf9 cells results in internalization of CP-P-GFP, visualized by confocal microscopy, signalizing a functional gut receptor Acyrthosiphon pisum

Localization

Localization Comment Organism GeneOntology No. Textmining
brush border membrane vesicle
-
 Acyrthosiphon pisum
-
-
membrane
-
 Acyrthosiphon pisum 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required Acyrthosiphon pisum

Organism

Organism UniProt Comment Textmining
Acyrthosiphon pisum Q1W3E8
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme APN from Spodoptera frugiperda SF9 cells by nickel affinity chromatography Acyrthosiphon pisum

Source Tissue

Source Tissue Comment Organism Textmining
gut
-
 Acyrthosiphon pisum
-

Synonyms

Synonyms Comment Organism
APN
-
 Acyrthosiphon pisum
membrane alanyl aminopeptidase N
-
 Acyrthosiphon pisum

General Information

General Information Comment Organism
physiological function membrane alanyl aminopeptidase N (APN) is a receptor for pea enation mosaic virus (PEMV) coat protein (CP) in the gut of the pea aphid, Acyrthosiphon pisum. PEMV virions specifically bind to APN. Aphid gut-derived brush border membrane vesicles, binding of recombinant CP-P-GFP construct compete with binding of GBP3.1, a peptide previously demonstrated to bind to APN in the aphid gut and to impede PEMV uptake into the hemocoel. GBP3.1 and PEMV bind to and compete for the same APN receptor Acyrthosiphon pisum