Cloned (Comment) | Organism |
---|---|
a 70 kDa fragment of the 106 kDa APN is expressed in Escherichia coli BL21-CodonPlus (DE3)/pRIL cells | Anopheles gambiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
brush border membrane | - |
Anopheles gambiae | 31526 | - |
membrane | - |
Anopheles gambiae | 16020 | - |
microvillus | microvilli of the posterior midgut | Anopheles gambiae | 5902 | - |
vesicle | - |
Anopheles gambiae | 31982 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | the protein has a zinc-binding motif (HEIAH) | Anopheles gambiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
106000 | - |
gel filtration | Anopheles gambiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Anopheles gambiae | APN is a specific binding protein, and a putative midgut receptor, for Bacillus thuringiensis Cry11Ba toxin | ? | - |
? | |
additional information | Anopheles gambiae CDC G3 | APN is a specific binding protein, and a putative midgut receptor, for Bacillus thuringiensis Cry11Ba toxin | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anopheles gambiae | C5H690 | - |
- |
Anopheles gambiae CDC G3 | C5H690 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Anopheles gambiae |
Purification (Comment) | Organism |
---|---|
HiTrap Ni2+-chelating HP column chromatography | Anopheles gambiae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
midgut | - |
Anopheles gambiae | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala-p-nitroanilide + H2O | - |
Anopheles gambiae | L-Ala + p-nitroaniline | - |
? | |
L-Ala-p-nitroanilide + H2O | - |
Anopheles gambiae CDC G3 | L-Ala + p-nitroaniline | - |
? | |
additional information | APN is a specific binding protein, and a putative midgut receptor, for Bacillus thuringiensis Cry11Ba toxin | Anopheles gambiae | ? | - |
? | |
additional information | APN is a specific binding protein, and a putative midgut receptor, for Bacillus thuringiensis Cry11Ba toxin | Anopheles gambiae CDC G3 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aminopeptidase N | - |
Anopheles gambiae |
APN2 | - |
Anopheles gambiae |