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Literature summary for 3.4.11.2 extracted from

  • Hatanaka, T.; Arima, J.; Uraji, M.; Uesugi, Y.; Iwabuchi, M.
    Characterization, cloning, sequencing, and expression of an aminopeptidase N from Streptomyces sp. TH-4 (2007), Appl. Microbiol. Biotechnol., 74, 347-356.
    View publication on PubMed
Search for more literature for 3.4.11.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, with His-tag Streptomyces sp.

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 0.005 mM, 5% residual activity Streptomyces sp.
antipain 0.074 mM, 46% residual activity Streptomyces sp.
bestatin 0.13 mM, 30% residual activity Streptomyces sp.
Cd2+ 1 mM, 9.2% residual activity Streptomyces sp.
chymostatin 0.1 mM, 35% residual activity Streptomyces sp.
EDTA 1 mM, 6% residual activity Streptomyces sp.
pefabloc 1 mM, 45% residual activity Streptomyces sp.
Zn2+ 1 mM, 36% residual activity Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 L-Lys-4-nitroanilide native enzyme, pH 7.0, 37°C Streptomyces sp.
0.11
-
 L-Lys-4-nitroanilide recombinant enzyme, pH 7.0, 37°C Streptomyces sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 1 mM, 149% of initial activity Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
94986
-
 1 * 95000, SDS-PAGE, 1 * 94986, calculated Streptomyces sp.
95000
-
 gel filtration Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-
Streptomyces sp. TH-4
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
culture fluid
-
 Streptomyces sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18.3
-
 pH 7.0, 37°C Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Boc-L-Lys-4-nitroanilide + H2O 21% of the activity with L-Lys-4-nitroanilide Streptomyces sp. Boc-L-Lys + 4-nitroaniline
-
 ?
L-Arg-4-nitroanilide + H2O 40% of the activity with L-Lys-4-nitroanilide Streptomyces sp. L-Arg + 4-nitroaniline
-
 ?
L-Arg-4-nitroanilide + H2O 40% of the activity with L-Lys-4-nitroanilide Streptomyces sp. TH-4 L-Arg + 4-nitroaniline
-
 ?
L-Leu-4-nitroanilide + H2O 9.5% of the activity with L-Lys-4-nitroanilide Streptomyces sp. L-Leu + 4-nitroaniline
-
 ?
L-Leu-4-nitroanilide + H2O 9.5% of the activity with L-Lys-4-nitroanilide Streptomyces sp. TH-4 L-Leu + 4-nitroaniline
-
 ?
L-Leu-L-Arg + H2O 50fold as active as L-Lys-L-Lys Streptomyces sp. L-Leu + L-Arg
-
 ?
L-Leu-L-Arg + H2O 50fold as active as L-Lys-L-Lys Streptomyces sp. TH-4 L-Leu + L-Arg
-
 ?
L-Leu-L-Met + H2O 49.4fold as active as L-Lys-L-Lys Streptomyces sp. L-Leu + L-Met
-
 ?
L-Leu-L-Met + H2O 49.4fold as active as L-Lys-L-Lys Streptomyces sp. TH-4 L-Leu + L-Met
-
 ?
L-Lys-4-nitroanilide + H2O
-
 Streptomyces sp. L-Lys + 4-nitroaniline
-
 ?
L-Lys-L-Arg + H2O 25.5fold as active as L-Lys-L-Lys Streptomyces sp. L-Lys + L-Arg
-
 ?
L-Lys-L-Lys + H2O
-
 Streptomyces sp. L-Lys
-
 ?
L-Met-4-nitroanilide + H2O 5.7% of the activity with L-Lys-4-nitroanilide Streptomyces sp. L-Met + 4-nitroaniline
-
 ?
L-Phe-4-nitroanilide + H2O 6.8% of the activity with L-Lys-4-nitroanilide Streptomyces sp. L-Phe + 4-nitroaniline
-
 ?
L-Phe-L-Arg + H2O 51.8fold as active as L-Lys-L-Lys Streptomyces sp. L-Phe + L-Arg
-
 ?
L-Phe-L-Phe + H2O 39.2fold as active as L-Lys-L-Lys Streptomyces sp. L-Phe
-
 ?
L-Tyr-L-Arg + H2O best substrate among dipeptides, 57.9fold as active as L-Lys-L-Lys Streptomyces sp. L-Tyr + L-Arg
-
 ?
additional information no hydrolysis of Lys-Pro and Pro-Phe bond Streptomyces sp. ?
-
 ?
additional information no hydrolysis of Lys-Pro and Pro-Phe bond Streptomyces sp. TH-4 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 95000, SDS-PAGE, 1 * 94986, calculated Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 40
-
 Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 5 min, 50% residual activity Streptomyces sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
18.7
-
 L-Lys-4-nitroanilide native enzyme, pH 7.0, 37°C Streptomyces sp.
21.3
-
 L-Lys-4-nitroanilide recombinant enzyme, pH 7.0, 37°C Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Streptomyces sp.