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Literature summary for 3.4.11.2 extracted from

  • Contreras-Rodriguez, A.; Ramirez-Zavala, B.; Contreras, A.; Schurig, G.G.; Sriranganathan, N.; Lopez-Merino, A.
    Purification and characterization of an immunogenic aminopeptidase of Brucella melitensis (2003), Infect. Immun., 71, 5238-5244.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.11.2

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 80% inhibition at 0.01 mM Brucella melitensis
bestatin 55% inhibition at 0.25 mM Brucella melitensis
EDTA complete inhibition at 2.5 mM Brucella melitensis
Hg2+ complete inhibition at 1 mM Brucella melitensis
Mg2+ 14% inhibition at 1 mM Brucella melitensis
additional information no or poor inhibition by leupeptin, PMSF, E64, and pepstatin Brucella melitensis
pefabloc 40% inhibition at 0.005 mM Brucella melitensis
Zn2+ complete inhibition at 1 mM Brucella melitensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 Lys-4-nitroanilide pH 7.0, 37°C Brucella melitensis
0.35
-
 Ala-4-nitroanilide pH 7.0, 37°C Brucella melitensis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information metallopeptidase, no or poor inhibition by Co2+ and Mg2+ Brucella melitensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
96000
-
 1 * 96000, SDS-PAGE Brucella melitensis
97000
-
 gel filtration Brucella melitensis

Organism

Organism UniProt Comment Textmining
Brucella melitensis Q8YG38 strain VTRM1, gene pepN
-
Brucella melitensis 16M Q8YG38 strain VTRM1, gene pepN
-

Purification (Commentary)

Purification (Comment) Organism
144fold Brucella melitensis

Storage Stability

Storage Stability Organism
-20°C, purified enzyme, 6 months, loss of 50% activity Brucella melitensis
-70°C, purified enzyme, 6 months, no loss of activity Brucella melitensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-4-nitroanilide + H2O
-
 Brucella melitensis Ala + 4-nitroaniline
-
 ?
Ala-4-nitroanilide + H2O
-
 Brucella melitensis 16M Ala + 4-nitroaniline
-
 ?
Lys-4-nitroanilide + H2O
-
 Brucella melitensis Lys + 4-nitroaniline
-
 ?
Lys-4-nitroanilide + H2O
-
 Brucella melitensis 16M Lys + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 96000, SDS-PAGE Brucella melitensis

Synonyms

Synonyms Comment Organism
aminopeptidase N
-
 Brucella melitensis
Ape
-
 Brucella melitensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Brucella melitensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 60 decreased activity above 50°C, 15% of maximal activity at 60°C Brucella melitensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 loss of 95% activity within 30 min Brucella melitensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Brucella melitensis

pH Range

pH Minimum pH Maximum Comment Organism
6 9 no activity below pH 6.0 and above pH 9.0 Brucella melitensis

pI Value

Organism Comment pI Value Maximum pI Value
Brucella melitensis isoelectric chromatofocusing
-
 4.8