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Literature summary for 3.4.11.2 extracted from

  • Wachsmuth, E.D.; Fritze, I.; Pfleiderer, G.
    An aminopeptidase occurring in pig kidney. I. An improved method of preparation. Physical and enzymic properties (1966), Biochemistry, 5, 169-174.
    View publication on PubMed
Search for more literature for 3.4.11.2

General Stability

General Stability Organism
6 M urea, no denaturation after 100 h, rapid denaturation at higher concentrations Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2 1 Leu-p-nitroanilide Km is pH-dependent between pH 6 and pH 9, maximum affinity at pH 7.0-7.3 Sus scrofa
0.6 6 Ala-p-nitroanilide Km is pH-dependent between pH 6 and pH 9, maximum affinity at pH 7.0-7.3 Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Sus scrofa
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
280000
-
 gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
aminoacyl peptide Sus scrofa
-
 ?
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone 35 vol.%, no denaturation after 100 h, rapid denaturation at higher concentrations Sus scrofa
chloroform saturated solution in H2O, no denaturation after 100 h, rapid denaturation at higher concentrations Sus scrofa
toluene saturated solution in H2O, no denaturation after 100 h, rapid denaturation at higher concentrations Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-p-nitroanilide + H2O
-
 Sus scrofa Ala + p-nitroaniline
-
 ?
aminoacyl peptide
-
 Sus scrofa ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 substrate Leu-p-nitroanilide Sus scrofa
70
-
 substrate Gly-p-nitroanilide Sus scrofa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 stable Sus scrofa
70
-
 50% loss of activity after 10 min Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
174
-
 Leu-p-nitroanilide
-
 Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9 maximum rate of hydrolysis of Leu-p-nitroanilide or Ala-p-nitroanilide, maximum substrate affinity at pH 7.0-7.3 Sus scrofa

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 11 stable for at least 3 h Sus scrofa