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Literature summary for 3.4.11.2 extracted from

  • Exterkate, F.A.; De Jong.M.; De Veer G.J.C.M.; Baankreis, R.
    Location and characterization of aminopeptidase N in Lactococcus lactis subsp. cremoris HP (1992), Appl. Microbiol. Biotechnol., 37, 46-54.
No PubMed abstract available
Search for more literature for 3.4.11.2

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol inhibits at high concentrations, 40-42% inhibition at 10 mM Lactococcus lactis
EDTA
-
 Lactococcus lactis
iodoacetamide 9-11% inhibition at 2 mM Lactococcus lactis
iodoacetic acid 100% inhibition at 2 mM Lactococcus lactis
Mn2+ not inhibitory Lactococcus lactis
N-ethylmaleimide 43-58% inhibition at 2 mM Lactococcus lactis
Zn2+
-
 Lactococcus lactis

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Lactococcus lactis 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ no reactivation after inhibition by EDTA Lactococcus lactis
Co2+ reactivation after inhibition by EDTA Lactococcus lactis
Mn2+ reactivation after inhibition by EDTA Lactococcus lactis
Ni2+ no reactivation after inhibition by EDTA Lactococcus lactis
Zn2+ reactivation after inhibition by EDTA Lactococcus lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
95000
-
 SDS-PAGE Lactococcus lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
aminoacyl peptide Lactococcus lactis
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aminoacyl peptide
-
 Lactococcus lactis ?
-
 ?
aminoacyl peptide + H2O release of positively charged and uncharged amino acids from N-terminus, unable to cleave peptide bond on N-terminal side of proline residue, oligopeptides hydrolyzed faster than tri- or dipeptides, highest activity with lysyl derivatives Lactococcus lactis alanyl peptides + H2O
-
 ?
Leu-p-nitroanilide + H2O
-
 Lactococcus lactis Leu + p-nitroaniline
-
 ?
Lys-p-nitroanilide + H2O
-
 Lactococcus lactis Lys + p-nitroaniline
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37 40
-
 Lactococcus lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.2
-
 Lactococcus lactis