Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
presence of a unique C-H to S hydrogen bond between the enzyme and its N-terminal-methionine polypeptide substrate, which allows specific interaction between apparent apolar interfaces, imposing a strict substrate recognition specificity and efficient catalysis | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
C105L | inactive | Mycobacterium tuberculosis |
C105S | 10fold decrease in kcat/Km value compared to wild-type | Mycobacterium tuberculosis |
C105T | 4old decrease in kcat/Km value compared to wild-type | Mycobacterium tuberculosis |
C105V | 6fold decrease in kcat/Km value compared to wild-type | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.94 | - |
L-Met 4-nitroanilide | mutant C105V, pH 7.5, 30°C | Mycobacterium tuberculosis | |
0.98 | - |
L-Met 4-nitroanilide | wild-type, pH 7.5, 30°C | Mycobacterium tuberculosis | |
2.47 | - |
L-Met 4-nitroanilide | mutant C105T, pH 7.5, 30°C | Mycobacterium tuberculosis | |
2.77 | - |
L-Met 4-nitroanilide | mutant C105S, pH 7.5, 30°C | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WK19 | - |
- |
Mycobacterium tuberculosis ATCC 25618 | P9WK19 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Met 4-nitroanilide + H2O | - |
Mycobacterium tuberculosis | L-Met + 4-nitroaniline | - |
? | |
L-Met 4-nitroanilide + H2O | - |
Mycobacterium tuberculosis ATCC 25618 | L-Met + 4-nitroaniline | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
3.3 degrees C increase in melting temperature for methionine bound protein compared to its norleucine homolog | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.59 | - |
L-Met 4-nitroanilide | mutant C105V, pH 7.5, 30°C | Mycobacterium tuberculosis | |
2.36 | - |
L-Met 4-nitroanilide | mutant C105S, pH 7.5, 30°C | Mycobacterium tuberculosis | |
6.3 | - |
L-Met 4-nitroanilide | mutant C105T, pH 7.5, 30°C | Mycobacterium tuberculosis | |
10.58 | - |
L-Met 4-nitroanilide | wild-type, pH 7.5, 30°C | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.85 | - |
L-Met 4-nitroanilide | mutant C105S, pH 7.5, 30°C | Mycobacterium tuberculosis | |
1.69 | - |
L-Met 4-nitroanilide | mutant C105V, pH 7.5, 30°C | Mycobacterium tuberculosis | |
2.54 | - |
L-Met 4-nitroanilide | mutant C105T, pH 7.5, 30°C | Mycobacterium tuberculosis | |
10.79 | - |
L-Met 4-nitroanilide | wild-type, pH 7.5, 30°C | Mycobacterium tuberculosis |