Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.11.18 extracted from

  • Bhat, S.Y.; Dey, A.; Qureshi, I.A.
    Structural and functional highlights of methionine aminopeptidase 2 from Leishmania donovani (2018), Int. J. Biol. Macromol., 115, 940-954 .
    View publication on PubMed
Search for more literature for 3.4.11.18

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Leishmania donovani

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modelling of MAP2 and molecular docking studies Leishmania donovani

Inhibitors

Inhibitors Comment Organism Structure
bestatin competitive Leishmania donovani

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.125
-
 L-alanine-4-methylcoumarin-7-amide presence of Ca2+, pH 8.0, 37°C Leishmania donovani
0.154
-
 L-alanine-4-methylcoumarin-7-amide presence of Co2+, pH 8.0, 37°C Leishmania donovani
0.167
-
 L-alanine-4-methylcoumarin-7-amide presence of Mn2+, pH 8.0, 37°C Leishmania donovani
0.172
-
 L-alanine-4-methylcoumarin-7-amide presence of Cu2+, pH 8.0, 37°C Leishmania donovani
0.179
-
 L-alanine-4-methylcoumarin-7-amide presence of Mg2+, pH 8.0, 37°C Leishmania donovani
0.284
-
 L-alanine-4-methylcoumarin-7-amide presence of Ni2+, pH 8.0, 37°C Leishmania donovani

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ highest activtiy in presence of Co2+ or Ca2+ Leishmania donovani
Co2+ highest activtiy in presence of Co2+ or Ca2+ Leishmania donovani
Cu2+
-
 Leishmania donovani
Mg2+
-
 Leishmania donovani
Mn2+
-
 Leishmania donovani
additional information Zn(II)-activated MAP2 shows negligible amidolytic activity Leishmania donovani
Ni2+
-
 Leishmania donovani

Organism

Organism UniProt Comment Textmining
Leishmania donovani A0A3S7WWI2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanine-4-methylcoumarin-7-amide + H2O
-
 Leishmania donovani L-alanine + 7-amino-4-methylcoumarin
-
 ?
L-leucine-4-methylcoumarin-7-amide + H2O
-
 Leishmania donovani L-leucine + 7-amino-4-methylcoumarin
-
 ?
L-methionine-4-methylcoumarin-7-amide + H2O
-
 Leishmania donovani L-methionine + 7-amino-4-methylcoumarin
-
 ?
additional information among the 4-methylcoumarin-7-amides tested, activity is highest with L-alanine 4-methylcoumarin-7-amide Leishmania donovani ?
-
 ?

Subunits

Subunits Comment Organism
? x * 51300, calculated from sequence Leishmania donovani

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.43
-
 L-alanine-4-methylcoumarin-7-amide presence of Cu2+, pH 8.0, 37°C Leishmania donovani
1.47
-
 L-alanine-4-methylcoumarin-7-amide presence of Ni2+, pH 8.0, 37°C Leishmania donovani
1.5
-
 L-alanine-4-methylcoumarin-7-amide presence of Ca2+, pH 8.0, 37°C Leishmania donovani
1.66
-
 L-alanine-4-methylcoumarin-7-amide presence of Mn2+, pH 8.0, 37°C Leishmania donovani
1.82
-
 L-alanine-4-methylcoumarin-7-amide presence of Mg2+, pH 8.0, 37°C Leishmania donovani
2.13
-
 L-alanine-4-methylcoumarin-7-amide presence of Co2+, pH 8.0, 37°C Leishmania donovani

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Leishmania donovani

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.086
-
 bestatin pH 8.0, 37°C Leishmania donovani

pI Value

Organism Comment pI Value Maximum pI Value
Leishmania donovani calculated from sequence
-
 6.6

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5
-
 L-alanine-4-methylcoumarin-7-amide presence of Cu2+, pH 8.0, 37°C Leishmania donovani
5.17
-
 L-alanine-4-methylcoumarin-7-amide presence of Ni2+, pH 8.0, 37°C Leishmania donovani
9.8
-
 L-alanine-4-methylcoumarin-7-amide presence of Mn2+, pH 8.0, 37°C Leishmania donovani
10
-
 L-alanine-4-methylcoumarin-7-amide presence of Mg2+, pH 8.0, 37°C Leishmania donovani
12
-
 L-alanine-4-methylcoumarin-7-amide presence of Ca2+, pH 8.0, 37°C Leishmania donovani
13.83
-
 L-alanine-4-methylcoumarin-7-amide presence of Co2+, pH 8.0, 37°C Leishmania donovani