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Literature summary for 3.4.11.18 extracted from

  • Calcagno, S.; Klein, C.D.
    N-Terminal methionine processing by the zinc-activated Plasmodium falciparum methionine aminopeptidase 1b (2016), Appl. Microbiol. Biotechnol., 100, 7091-7102 .
    View publication on PubMed
Search for more literature for 3.4.11.18

Application

Application Comment Organism
synthesis the covalently immobilized enzyme bound to iminodiacetic acid-agarose or chloroacetamido-hexyl-agarose shows long-term stability and allows a continuous, heterogenous processing of N-terminal methionines, for example, in recombinant proteins. Activation by zinc avoids the introduction of heavy metals with toxicological liabilities and oxidative potential into biotechnological processes Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
C316S 51% of wild-type activity in presence of Zn2+ Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.061
-
 Met-Gly-Met-Met presence of 0.1 mM Co2+, pH 7.5, 37°C Plasmodium falciparum
1.662
-
 Met-Gly-Met-Met presence of 0.1 mM Zn2+, pH 7.5, 37°C Plasmodium falciparum

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ enzyme is reliably activated by zinc ions Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum KT943909 synthetic construct for expression in Escherichia coli
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Met-Gly-Met-Met + H2O
-
 Plasmodium falciparum Gly-Met-Met + Met
-
 ?
Met-Gly-Met-Phe + H2O
-
 Plasmodium falciparum Gly-Met-Phe + Met
-
 ?
Nle-Gly-Met-Phe + H2O zinc-activated einzyme shows 159% of the activity with substrate Nle-Gly-Met-Phe Plasmodium falciparum Gly-Met-Phe + norleucine
-
 ?

Subunits

Subunits Comment Organism
? x * 59868, calculated from sequence Plasmodium falciparum

Synonyms

Synonyms Comment Organism
methionine aminopeptidase 1b
-
 Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.07
-
 Met-Gly-Met-Met presence of 0.1 mM Zn2+, pH 7.5, 37°C Plasmodium falciparum
9.13
-
 Met-Gly-Met-Met presence of 0.1 mM Co2+, pH 7.5, 37°C Plasmodium falciparum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.65
-
 Met-Gly-Met-Met presence of 0.1 mM Zn2+, pH 7.5, 37°C Plasmodium falciparum
8.6
-
 Met-Gly-Met-Met presence of 0.1 mM Co2+, pH 7.5, 37°C Plasmodium falciparum