Crystallization (Comment) | Organism |
---|---|
wild-type enzyme crystals are soaked with N-ethylmaleimide, 2-hydroxyethyl disulfide, and methyl homocysteine disulfide. Crystal structure determined in the presence of cobalt chloride shows electron density for the mercaptoethanol covalently linked to Cys105. However, in the absence of cobalt chloride, the whole 2-hydroxyethyl disulfide molecule is observed in the S1 pocket without any covalent modification. Metal ions in the active site assist in the covalent modification of Cys105 by orienting the reagents appropriately for a successful reaction | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
C105S | contratry to wild-type, no inhibition by cysteien-modifying compounds N-ethylmaleimide, 5,5'-dithiobis(2-nitrobenzoic acid) and 2-hydroxyethyl disulfide | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-hydroxyethyl disulfide | inhibition is through covalent modifiction of residue C105 | Mycobacterium tuberculosis | |
5,5'-dithiobis(2-nitrobenzoic acid) | inhibition is through covalent modifiction of residue C105 | Mycobacterium tuberculosis | |
N-ethylmaleimide | 1 mM, 90% inhibition. Inhibition is through covalent modifiction of residue C105 | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WK19 | - |
- |
Mycobacterium tuberculosis ATCC 25618 | P9WK19 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
MetAP1c | - |
Mycobacterium tuberculosis |
methionine aminopeptidase 2 | - |
Mycobacterium tuberculosis |