Literature summary for 3.4.11.18 extracted from

Li, J.Y.; Chen, L.L.; Cui, Y.M.; Luo, Q.L.; Gu, M.; Nan, F.J.; Ye, Q.Z.
Characterization of full length and truncated type I human methionine aminopeptidases expressed from Escherichia coli (2004), Biochemistry, 43, 7892-7898.

Search for more literature for 3.4.11.18

Protein Variants

Protein Variants	Comment	Organism
additional information	truncated mutant lacking N-terminal 66 amino acids, 2.5fold increase in kcat value. Truncated mutant lacking N-terminal 135 amino acids, 4fold increase in KM-value	Homo sapiens

General Stability

General Stability	Organism
recombinant full-lenth enzyme is not stable during purification and storage at 4°C, and a smaller but active protein appears that corresponds to N-terminally truncated protein lacking amino acids 1-66	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
3-((4-methylpent-3-enoyl)amino)-N-1,3-thiazol-2-ylpyridine-2-carboxamide	wild-type, 50% inhibition at 0.0149 mM, mutant lacking N-terminal 66 amino acids, 50% inhibition at 0.00068 mM	Homo sapiens
3-((5-methylhex-4-enoyl)amino)-N-1,3-thiazol-2-ylpyridine-2-carboxamide	wild-type, 50% inhibition at 0.0074 mM, mutant lacking N-terminal 66 amino acids, 50% inhibition at 0.0010 mM	Homo sapiens
3-((cyclopent-1-en-1-ylcarbonyl)amino)-N-1,3-thiazol-2-ylpyridine-2-carboxamide	wild-type, 50% inhibition above 100 mM, mutant lacking N-terminal 66 amino acids, 50% inhibition above 100 mM	Homo sapiens
3-(benzoylamino)-N-1,3-thiazol-2-ylpyridine-2-carboxamide	wild-type, 50% inhibition above 100 mM, mutant lacking N-terminal 66 amino acids, 50% inhibition at 0.0074 mM	Homo sapiens
3-(hexanoylamino)-N-1,3-thiazol-2-ylpyridine-2-carboxamide	wild-type, 50% inhibition at 0.0867 mM, mutant lacking N-terminal 66 amino acids, 50% inhibition at 0.0020 mM	Homo sapiens
3-(pent-4-enoylamino)-N-1,3-thiazol-2-ylpyridine-2-carboxamide	wild-type, 50% inhibition above 0.1 mM, mutant lacking N-terminal 66 amino acids, 50% inhibition at 0.00079 mM	Homo sapiens
3-hydroxy-N-1,3-thiazol-2-ylpyridine-2-carboxamide	wild-type, 50% inhibition at 0.0081 mM, mutant lacking N-terminal 66 amino acids, 50% inhibition at 0.0035 mM	Homo sapiens
isobutyl (2-((1,3-thiazol-2-ylamino)carbonyl)pyridin-3-yl)carbamate	wild-type, 50% inhibition above 100 mM, mutant lacking N-terminal 66 amino acids, 50% inhibition above 100 mM	Homo sapiens
N-1,3-thiazol-2-ylpyridine-2-carboxamide	wild-type, 50% inhibition at 0.0104 mM, mutant lacking N-terminal 66 amino acids, 50% inhibition at 0.0049 mM	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.28	-	L-Met-PSI[C(O)S]Gly-L-Phe	N-terminally truncated mutant, lacking 66 amino acids, pH 7.0, 22°C	Homo sapiens
1.88	-	L-Met-PSI[C(O)S]Gly-L-Phe	wild-type, pH 7.0, 22°C	Homo sapiens
8.81	-	L-Met-PSI[C(O)S]Gly-L-Phe	N-terminally truncated mutant lacking 135 amino acids, pH 7.0, 22°C	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
44199	-	x * 44199, MALDI-TOF, x * 44207, calculated	Homo sapiens
44207	-	x * 44199, MALDI-TOF, x * 44207, calculated	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	type I enzyme, expressed in Escherichia coli	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.3	-	substrate L-Nle-L-Ala-L-Ser, N-terminally truncated mutant lacking 135 amino acids, pH 7.0, 22°C	Homo sapiens
7.3	-	substrate L-Thr-PSI[C(O)S]Gly-L-Phe, wild-type, pH 7.0, 22°C	Homo sapiens
8.8	-	substrate L-Thr-PSI[C(O)S]Gly-L-Phe, N-terminally truncated mutant lacking 135 amino acids, pH 7.0, 22°C	Homo sapiens
9.4	-	substrate L-Ala-PSI[C(O)S]Gly-L-Phe, N-terminally truncated mutant lacking 135 amino acids, pH 7.0, 22°C	Homo sapiens
12.1	-	substrate L-Thr-PSI[C(O)S]Gly-L-Phe, N-terminally truncated mutant, lacking 66 amino acids, pH 7.0, 22°C	Homo sapiens
18.4	-	substrate L-Met-PSI[C(O)S]Gly-L-Phe, N-terminally truncated mutant lacking 135 amino acids, pH 7.0, 22°C	Homo sapiens
22	-	substrate L-Phe-L-Ala-L-Ser-L-Trp, N-terminally truncated mutant, lacking 66 amino acids, pH 7.0, 22°C	Homo sapiens
23	-	substrate L-Leu-L-Ala-L-Ser-L-Trp, N-terminally truncated mutant, lacking 66 amino acids, pH 7.0, 22°C	Homo sapiens
30	-	substrate L-Leu-L-Ala-L-Ser-L-Trp, wild-type, pH 7.0, 22°C	Homo sapiens
30	-	substrate L-Phe-L-Ala-L-Ser-L-Trp, wild-type, pH 7.0, 22°C	Homo sapiens
42.7	-	substrate L-Met-PSI[C(O)S]Gly-L-Phe, wild-type, pH 7.0, 22°C	Homo sapiens
100	-	substrate L-Met-L-Ala-L-Ser, N-terminally truncated mutant, lacking 66 amino acids, pH 7.0, 22°C	Homo sapiens
141	-	substrate L-Nle-L-Ala-L-Ser, N-terminally truncated mutant, lacking 66 amino acids, pH 7.0, 22°C	Homo sapiens
142	-	substrate L-Met-L-Ala-L-Ser, wild-type, pH 7.0, 22°C	Homo sapiens
146	-	substrate L-Met-PSI[C(O)S]Gly-L-Phe, N-terminally truncated mutant, lacking 66 amino acids, pH 7.0, 22°C	Homo sapiens
183	-	substrate L-Nle-L-Ala-L-Ser, wild-type, pH 7.0, 22°C	Homo sapiens
201	-	substrate L-Met-L-Ala-L-Ser-L-Trp, N-terminally truncated mutant, lacking 66 amino acids, pH 7.0, 22°C	Homo sapiens
396	-	substrate L-Met-L-Ala-L-Ser-L-Trp, wild-type, pH 7.0, 22°C	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Ala-PSI[C(O)S]Gly-L-Phe + H2O	-	Homo sapiens	?	-	?
L-Leu-L-Ala-L-Ser-L-Trp + H2O	-	Homo sapiens	?	-	?
L-Met-L-Ala-L-Ser + H2O	-	Homo sapiens	?	-	?
L-Met-L-Ala-L-Ser-L-Trp + H2O	-	Homo sapiens	?	-	?
L-Met-PSI[C(O)S]Gly-L-Phe + H2O	-	Homo sapiens	?	-	?
L-Nle-L-Ala-L-Ser + H2O	-	Homo sapiens	?	-	?
L-Phe-L-Ala-L-Ser-L-Trp + H2O	-	Homo sapiens	?	-	?
L-Thr-PSI[C(O)S]Gly-L-Phe + H2O	-	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 44199, MALDI-TOF, x * 44207, calculated	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	L-Met-PSI[C(O)S]Gly-L-Phe	wild-type, pH 7.0, 22°C	Homo sapiens
0.97	-	L-Met-PSI[C(O)S]Gly-L-Phe	N-terminally truncated mutant, lacking 66 amino acids, pH 7.0, 22°C	Homo sapiens
6.47	-	L-Met-PSI[C(O)S]Gly-L-Phe	wild-type, pH 7.0, 22°C	Homo sapiens
7.31	-	L-Met-PSI[C(O)S]Gly-L-Phe	N-terminally truncated mutant lacking 135 amino acids, pH 7.0, 22°C	Homo sapiens
16.61	-	L-Met-PSI[C(O)S]Gly-L-Phe	N-terminally truncated mutant, lacking 66 amino acids, pH 7.0, 22°C	Homo sapiens

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Release 2023.1 (January 2023)