Crystallization (Comment) | Organism |
---|---|
crystal structure of MetAP-1 as an apoenzyme and its complexes with various 1,2,4-triazole-based derivatives at high-resolution. Form 1 crystals are grown in the presence of 0.1 mM NiCl2 with 25% PEG 3350, 100 mM bis-Tris, pH 6.5, 200 mM LiSO4. Form 2 crystals are grown in the absence of metal ions using 25% PEG, 100 mM bis-Tris, pH 5.5, 200 mM ammonium acetate as precipitating agent | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
MCB2379 | IC50: 599 nM in presence of Co2+, 1455 nM in presence of Zn2+ | Staphylococcus aureus | |
MCB2813 | IC50: 43.7 nM in presence of Co2+, 300 nM in presence of Zn2+ | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.75 | - |
Met-Pro-4-nitroanilide | pH 7.5, in presence of Mn2+ | Staphylococcus aureus | |
1.41 | - |
Met-Pro-4-nitroanilide | pH 7.5, in presence of Co2+ | Staphylococcus aureus | |
3.26 | - |
Met-Pro-4-nitroanilide | pH 7.5, in presence of Zn2+ | Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | optimal concentration is 0.008 mM | Staphylococcus aureus | |
Fe2+ | optimal concentration is 0.008 mM | Staphylococcus aureus | |
Ni2+ | enzyme is active at concentrations below 0.002 mM, inhibition at higher concentrations | Staphylococcus aureus | |
Zn2+ | optimal concentration | Staphylococcus aureus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
gel filtration | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Met-Pro-4-nitroanilide + H2O | - |
Staphylococcus aureus | Met + Pro-4-nitroanilide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | eukaryotes contain both the type I and the type II MetAPs, whereas prokaryotes possess only the type I enzyme | Staphylococcus aureus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.75 | - |
Met-Pro-4-nitroanilide | pH 7.5, in presence of Mn2+ | Staphylococcus aureus | |
2.67 | - |
Met-Pro-4-nitroanilide | pH 7.5, in presence of Zn2+ | Staphylococcus aureus | |
11.9 | - |
Met-Pro-4-nitroanilide | pH 7.5, in presence of Co2+ | Staphylococcus aureus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0000437 | 0.0003 | IC50: 43.7 nM in presence of Co2+, 300 nM in presence of Zn2+ | Staphylococcus aureus | MCB2813 | |
0.000599 | 0.001455 | IC50: 599 nM in presence of Co2+, 1455 nM in presence of Zn2+ | Staphylococcus aureus | MCB2379 |