Protein Variants | Comment | Organism |
---|---|---|
D219N | the mutant enzyme has 1000fold lower activity and a different substrate specificity when compared to wild-type enzyme. Expression of the mutant enzyme in yeast causes a slow-growth phenotype and interferes with wild-type metAP1 in a dominant manner. The mutant enzyme contains 0.43 mol of Co2+ per mol. The wild-type enzyme contains 0.75 mol of Co2+ per mol of enzyme. The mutant enzyme prefers substrates, having Ser or Pro at P1', over substrates that contain glycine and alanine at that position. The wild-type enzyme prefers substrates that have Ala, Gly, Ser or Pro at P1' | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.54 | - |
Met-Ala-Ser | pH 7.4, 37°C mutant enzyme D219N | Saccharomyces cerevisiae | |
13.9 | - |
Met-Gly-Met-Met | pH 7.4, 37°C mutant enzyme D219N | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | the mutant enzyme contains 0.43 mol of Co2+ per mol. The wild-type enzyme contains 0.75 mol of Co2+ per mol of enzyme | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
x * 43000, mutant enzyme D219N, SDS-PAGE | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
methionine aminopeptidase 1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Met-Ala-Ser + H2O | - |
Saccharomyces cerevisiae | Met + Ala-Ser | - |
? | |
Met-Gly-Met-Met + H2O | - |
Saccharomyces cerevisiae | Met + Gly-Met-Met | - |
? | |
Met-Pro-Gly + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
Met-Ser-Gly + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
additional information | the mutant enzyme prefers substrates, having Ser or Pro at P1', over substrates that contain glycine and alanine at that position. The wild-type enzyme prefers substrates that have Ala, Gly, Ser or Pro at P1'. Amino-terminal Met cannot be removed by either the mutant or wild-type enzyme from substrates having large and charged amino acids at P1' position | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000, mutant enzyme D219N, SDS-PAGE | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00325 | - |
Met-Ala-Ser | pH 7.4, 37°C mutant enzyme D219N | Saccharomyces cerevisiae | |
0.0427 | - |
Met-Gly-Met-Met | pH 7.4, 37°C mutant enzyme D219N | Saccharomyces cerevisiae |