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Literature summary for 3.4.11.18 extracted from

  • Zuo, S.; Guo, Q.; Ling, C.; Chang, Y.H.
    Evidence that two zinc fingers in the methionine aminopeptidase from Saccharomyces cerevisiae are important for normal growth (1995), Mol. Gen. Genet., 246, 247-253.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
C22S the activity with Met-Gly-Met-Met is 116% of that of the wild-type enzyme Saccharomyces cerevisiae
C27S the activity with Met-Gly-Met-Met is 107% of that of the wild-type enzyme Saccharomyces cerevisiae
C37S the activity with Met-Gly-Met-Met is 94% of that of the wild-type enzyme Saccharomyces cerevisiae
C40S the activity with Met-Gly-Met-Met is 85% of that of the wild-type enzyme Saccharomyces cerevisiae
C50S the activity with Met-Gly-Met-Met is 99% of that of the wild-type enzyme Saccharomyces cerevisiae
C54S the activity with Met-Gly-Met-Met is 97% of that of the wild-type enzyme Saccharomyces cerevisiae
DELTA2-69 1 mol of wild-type enzyme contains 2 mol of zinc ions and at least 1 mol of cobalt ion, whereas 1 mol of the truncated methionine aminopeptidase DELTA2-69 lacking the putative zinc fingers cotains only a trace amount of zinc ions but still contains one mole of cobalt ion,the activity with Met-Gly-Met-Met is 84% of that of the wild-type enzyme Saccharomyces cerevisiae
H62R the activity with Met-Gly-Met-Met is 95% of that of the wild-type enzyme Saccharomyces cerevisiae
H66R the activity with Met-Gly-Met-Met is 84% of that of the wild-type enzyme Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1
-
Met-Ala-Ser truncated enzyme Saccharomyces cerevisiae
2.1
-
Met-Ala-Ser 37°C truncated mutant enzyme DELTA2-69 Saccharomyces cerevisiae
2.67
-
Met-Ala-Ser 37°C, wild-type enzyme Saccharomyces cerevisiae
5
-
Met-Gly-Met-Met 37°C, truncated mutant enzyme DELTA2-69 Saccharomyces cerevisiae
6.56
-
Met-Gly-Met-Met 37°C, wild-type enzyme Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 1 mol of wild-type enzyme contains at least 1 mol of Co2+ Saccharomyces cerevisiae
Co2+ 1 mol of wild-type enzyme contains 2 mol of zinc ions and at least 1 mol of cobalt ion, whereas 1 mol of the truncated methionine aminopeptidase DELTA2-69 lacking the putative zinc fingers cotains only a trace amount of zinc ions but still contains one mol of cobalt ion Saccharomyces cerevisiae
Zinc 1 mol of wild-type enzyme contains 2 mol of Zn2+, zinc fingers are essential for normal MAP function Saccharomyces cerevisiae
Zn2+ 1 mol of wild-type enzyme contains 2 mol of zinc ions and at least 1 mol of cobalt ion, whereas 1 mol of the truncated methionine aminopeptidase DELTA2-69 lacking the putative zinc fingers cotains only a trace amount of zinc ions but still contains one mol of cobalt ion Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type enzyme and truncated enzyme form DELTA2-69 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Met-Ala-Ser + H2O
-
Saccharomyces cerevisiae Met + Ala-Ser
-
?
Met-Gly-Met-Met + H2O
-
Saccharomyces cerevisiae Gly-Met-Met + Met
-
?
Met-Gly-Met-Met + H2O
-
Saccharomyces cerevisiae Met + Gly-Met-Met
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
19.6
-
Met-Ala-Ser 37°C, wild-type enzyme Saccharomyces cerevisiae
23.6
-
Met-Gly-Met-Met 37°C, wild-type enzyme Saccharomyces cerevisiae