Protein Variants | Comment | Organism |
---|---|---|
C22S | the activity with Met-Gly-Met-Met is 116% of that of the wild-type enzyme | Saccharomyces cerevisiae |
C27S | the activity with Met-Gly-Met-Met is 107% of that of the wild-type enzyme | Saccharomyces cerevisiae |
C37S | the activity with Met-Gly-Met-Met is 94% of that of the wild-type enzyme | Saccharomyces cerevisiae |
C40S | the activity with Met-Gly-Met-Met is 85% of that of the wild-type enzyme | Saccharomyces cerevisiae |
C50S | the activity with Met-Gly-Met-Met is 99% of that of the wild-type enzyme | Saccharomyces cerevisiae |
C54S | the activity with Met-Gly-Met-Met is 97% of that of the wild-type enzyme | Saccharomyces cerevisiae |
DELTA2-69 | 1 mol of wild-type enzyme contains 2 mol of zinc ions and at least 1 mol of cobalt ion, whereas 1 mol of the truncated methionine aminopeptidase DELTA2-69 lacking the putative zinc fingers cotains only a trace amount of zinc ions but still contains one mole of cobalt ion,the activity with Met-Gly-Met-Met is 84% of that of the wild-type enzyme | Saccharomyces cerevisiae |
H62R | the activity with Met-Gly-Met-Met is 95% of that of the wild-type enzyme | Saccharomyces cerevisiae |
H66R | the activity with Met-Gly-Met-Met is 84% of that of the wild-type enzyme | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.1 | - |
Met-Ala-Ser | truncated enzyme | Saccharomyces cerevisiae | |
2.1 | - |
Met-Ala-Ser | 37°C truncated mutant enzyme DELTA2-69 | Saccharomyces cerevisiae | |
2.67 | - |
Met-Ala-Ser | 37°C, wild-type enzyme | Saccharomyces cerevisiae | |
5 | - |
Met-Gly-Met-Met | 37°C, truncated mutant enzyme DELTA2-69 | Saccharomyces cerevisiae | |
6.56 | - |
Met-Gly-Met-Met | 37°C, wild-type enzyme | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mol of wild-type enzyme contains at least 1 mol of Co2+ | Saccharomyces cerevisiae | |
Co2+ | 1 mol of wild-type enzyme contains 2 mol of zinc ions and at least 1 mol of cobalt ion, whereas 1 mol of the truncated methionine aminopeptidase DELTA2-69 lacking the putative zinc fingers cotains only a trace amount of zinc ions but still contains one mol of cobalt ion | Saccharomyces cerevisiae | |
Zinc | 1 mol of wild-type enzyme contains 2 mol of Zn2+, zinc fingers are essential for normal MAP function | Saccharomyces cerevisiae | |
Zn2+ | 1 mol of wild-type enzyme contains 2 mol of zinc ions and at least 1 mol of cobalt ion, whereas 1 mol of the truncated methionine aminopeptidase DELTA2-69 lacking the putative zinc fingers cotains only a trace amount of zinc ions but still contains one mol of cobalt ion | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type enzyme and truncated enzyme form DELTA2-69 | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Met-Ala-Ser + H2O | - |
Saccharomyces cerevisiae | Met + Ala-Ser | - |
? | |
Met-Gly-Met-Met + H2O | - |
Saccharomyces cerevisiae | Gly-Met-Met + Met | - |
? | |
Met-Gly-Met-Met + H2O | - |
Saccharomyces cerevisiae | Met + Gly-Met-Met | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
19.6 | - |
Met-Ala-Ser | 37°C, wild-type enzyme | Saccharomyces cerevisiae | |
23.6 | - |
Met-Gly-Met-Met | 37°C, wild-type enzyme | Saccharomyces cerevisiae |