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Literature summary for 3.4.11.17 extracted from
- Crystallization and characterization of a novel aminopeptidase from Trichosporon cutaneum (1983), Agric. Biol. Chem., 47, 2483-2493.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | part of method for Trp-production | Cutaneotrichosporon cutaneum |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Cutaneotrichosporon cutaneum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 27% inhibition at 1 mM | Cutaneotrichosporon cutaneum |  |
| alpha,alpha'-dipyridyl | 53% inhibition at 1 mM | Cutaneotrichosporon cutaneum | |
| monoiodoacetate | 25% inhibition at 1 mM | Cutaneotrichosporon cutaneum | |
| N-ethylmaleimide | 50% inhibition at 1 mM | Cutaneotrichosporon cutaneum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.6 | - |
Trp-amide | - |
Cutaneotrichosporon cutaneum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Cutaneotrichosporon cutaneum | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | can partially replace manganese | Cutaneotrichosporon cutaneum | |
| Mg2+ | can partially replace manganese, after long pre-incubation | Cutaneotrichosporon cutaneum | |
| Mn2+ | required | Cutaneotrichosporon cutaneum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
4 * 68000, SDS-PAGE | Cutaneotrichosporon cutaneum |
| 270000 | - |
gel filtration | Cutaneotrichosporon cutaneum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cutaneotrichosporon cutaneum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cutaneotrichosporon cutaneum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1110 | - |
- |
Cutaneotrichosporon cutaneum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ala-amide + H2O | - |
Cutaneotrichosporon cutaneum | Ala + NH3 | - |
ir | |
| Asn + H2O | - |
Cutaneotrichosporon cutaneum | Asp + NH3 | - |
ir | |
| citrulline + H2O | - |
Cutaneotrichosporon cutaneum | ? + ? | - |
ir | |
| Gln + H2O | - |
Cutaneotrichosporon cutaneum | Glu + NH3 | - |
ir | |
| His-Ala + H2O | - |
Cutaneotrichosporon cutaneum | His + Ala | - |
ir | |
| His-Gly + H2O | - |
Cutaneotrichosporon cutaneum | His + Gly | - |
ir | |
| His-Leu + H2O | - |
Cutaneotrichosporon cutaneum | His + Leu | - |
ir | |
| His-Phe + H2O | - |
Cutaneotrichosporon cutaneum | His + Phe | - |
ir | |
| Leu-Ala + H2O | - |
Cutaneotrichosporon cutaneum | Leu + Ala | - |
ir | |
| Leu-amide + H2O | - |
Cutaneotrichosporon cutaneum | Leu + NH3 | - |
ir | |
| Leu-Gly + H2O | - |
Cutaneotrichosporon cutaneum | Leu + Gly | - |
ir | |
| Leu-Gly-Gly + H2O | - |
Cutaneotrichosporon cutaneum | Leu + Gly-Gly | - |
ir | |
| Leu-Phe + H2O | - |
Cutaneotrichosporon cutaneum | Leu + Phe | - |
ir | |
| Leu-Trp + H2O | - |
Cutaneotrichosporon cutaneum | Leu + Trp | - |
ir | |
| Met-amide + H2O | - |
Cutaneotrichosporon cutaneum | Met + NH3 | - |
ir | |
| additional information | generally relatively high affinity towards peptides with N-terminal Trp | Cutaneotrichosporon cutaneum | ? | - |
? | |
| Phe-amide + H2O | - |
Cutaneotrichosporon cutaneum | Phe + NH3 | - |
ir | |
| Phe-Leu + H2O | - |
Cutaneotrichosporon cutaneum | Phe + Leu | - |
ir | |
| Trp-Ala + H2O | - |
Cutaneotrichosporon cutaneum | Trp + Ala | - |
ir | |
| Trp-amide + H2O | - |
Cutaneotrichosporon cutaneum | Trp + NH3 | - |
ir | |
| Trp-Glu + H2O | - |
Cutaneotrichosporon cutaneum | Trp + Glu | - |
ir | |
| Trp-Gly + H2O | - |
Cutaneotrichosporon cutaneum | Trp + Gly | - |
ir | |
| Trp-Leu + H2O | - |
Cutaneotrichosporon cutaneum | Trp + Leu | - |
ir | |
| Trp-Phe + H2O | - |
Cutaneotrichosporon cutaneum | Trp + Phe | - |
ir | |
| Trp-Trp + H2O | - |
Cutaneotrichosporon cutaneum | Trp + Trp | - |
ir | |
| Trp-Tyr + H2O | - |
Cutaneotrichosporon cutaneum | Trp + Tyr | - |
ir | |
| Tyr-amide + H2O | - |
Cutaneotrichosporon cutaneum | Tyr + NH3 | - |
ir | |
| Val-Ala + H2O | - |
Cutaneotrichosporon cutaneum | Val + Ala | - |
ir | |
| Val-amide + H2O | - |
Cutaneotrichosporon cutaneum | Val + NH3 | - |
ir | |
| Val-Gly-Gly + H2O | - |
Cutaneotrichosporon cutaneum | Val + Gly-Gly | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 68000, SDS-PAGE | Cutaneotrichosporon cutaneum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 45 | - |
Cutaneotrichosporon cutaneum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
below | Cutaneotrichosporon cutaneum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 9.5 | - |
Cutaneotrichosporon cutaneum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
N-ethylmaleimide | - |
Cutaneotrichosporon cutaneum | |
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