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Literature summary for 3.4.11.14 extracted from

  • Sengupta, S.; Horowitz, P.M.; Karsten, S.L.; Jackson, G.R.; Geschwind, D.H.; Fu, Y.; Berry, R.W.; Binder, L.I.
    Degradation of tau protein by puromycin-sensitive aminopeptidase in vitro (2006), Biochemistry, 45, 15111-15119.
    View publication on PubMed
Search for more literature for 3.4.11.14

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL-21 (DE3) cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
anti-PSA immunglobulinb G 53.4% residual activity at 0.1 mg/ml Homo sapiens
Aprotinin 82% residual activity at 0.1 mM Homo sapiens
bestatin 2.57% residual activity at 0.1 mM Homo sapiens
EDTA 44% residual activity at 0.5 mM Homo sapiens
leupeptin 83% residual activity at 0.1 mM Homo sapiens
additional information not inhibited by phenylmethylsulfonylfluoride Homo sapiens
puromycin 3.12% residual activity at 0.1 mM Homo sapiens
Zn2+ 79.4% residual activity at 0.01 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
 Val-p-nitroanilide with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C Homo sapiens
0.25
-
 Leu-p-nitroanilide with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C Homo sapiens
0.27
-
 Ala-p-nitroanilide with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C Homo sapiens
0.47
-
 Pro-p-nitroanilide with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C Homo sapiens
0.8
-
 Met-p-nitroanilide with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C Homo sapiens
2.2
-
 Lys-p-nitroanilide with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 9.8% increase of activity at 5 mM Homo sapiens
Mg2+ 26% increase of activity at 1 mM Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione Sepharose column chromatography and Superdex-200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-p-nitroanilide + H2O
-
 Homo sapiens Ala + p-nitroaniline
-
 ?
Leu-p-nitroanilide + H2O
-
 Homo sapiens Leu + p-nitroaniline
-
 ?
Lys-p-nitroanilide + H2O
-
 Homo sapiens Lys + p-nitroaniline
-
 ?
Met-p-nitroanilide + H2O
-
 Homo sapiens Met + p-nitroaniline
-
 ?
Pro-p-nitroanilide + H2O
-
 Homo sapiens Pro + p-nitroaniline
-
 ?
Val-p-nitroanilide + H2O
-
 Homo sapiens Val + p-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
PSA
-
 Homo sapiens
puromycin-sensitive aminopeptidase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.43
-
 Val-p-nitroanilide with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C Homo sapiens
3.5
-
 Leu-p-nitroanilide with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C Homo sapiens
3.8
-
 Ala-p-nitroanilide with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C Homo sapiens
16.9
-
 Pro-p-nitroanilide with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C Homo sapiens
19.83
-
 Leu-p-nitroanilide with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C Homo sapiens
20.83
-
 Ala-p-nitroanilide with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C Homo sapiens
23.17
-
 Pro-p-nitroanilide with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C Homo sapiens
30
-
 Met-p-nitroanilide with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C Homo sapiens
89
-
 Lys-p-nitroanilide with 1 mM dithiothreitol, in 10 mM Tris-HCl, pH 7.4, at 37°C Homo sapiens