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Literature summary for 3.4.11.14 extracted from
- Human kidney alanine aminopeptidase: physical and kinetic properties of a sialic acid containing glycoprotein (1978), Biochemistry, 17, 2990-2994.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | - |
Met-2-naphthylamide | - |
Homo sapiens |  |
| 0.04 | - |
Lys-2-naphthylamide | - |
Homo sapiens | |
| 0.042 | - |
Val-2-naphthylamide | - |
Homo sapiens | |
| 0.048 | - |
Ile-naphthylamide | - |
Homo sapiens | |
| 0.054 | - |
Arg-2-naphthylamide | - |
Homo sapiens | |
| 0.071 | - |
Leu-2-naphthylamide | - |
Homo sapiens | |
| 0.123 | - |
Ala-2-naphthylamide | - |
Homo sapiens | |
| 0.142 | - |
Phe-2-naphthylamide | - |
Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 1.65fold stimulation | Homo sapiens | |
| Zinc | the enzyme contains 1 mol of zinc per 113500 g of protein, the zinc is firmly bound | Homo sapiens | |
| Zinc | 1 mol Zn2+ for 113500 g protein, firmly bound, not removed by chelating agents | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 119500 | - |
2 * 119500, SDS-PAGE | Homo sapiens |
| 236000 | - |
sedimentation equilibrium centrifugation | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | contains 21% carbohydrate | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 76.7 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ala-2-naphthylamide + H2O | - |
Homo sapiens | Ala + 2-naphthylamine | - |
? | |
| Arg-2-naphthylamide + H2O | - |
Homo sapiens | Arg + 2-naphthylamine | - |
? | |
| Ile-2-naphthylamide + H2O | - |
Homo sapiens | Ile + 2-naphthylamide | - |
? | |
| Leu-2-naphthylamide + H2O | - |
Homo sapiens | Leu + 2-naphthylamine | - |
? | |
| Lys-2-naphthylamide + H2O | - |
Homo sapiens | Lys + 2-naphthylamine | - |
? | |
| Met-2-naphthylamide + H2O | - |
Homo sapiens | Met + 2-naphthylamine | - |
? | |
| Phe-2-naphthylamide + H2O | - |
Homo sapiens | Phe + 2-naphthylamine | - |
? | |
| Val-2-naphthylamide + H2O | - |
Homo sapiens | Val + 2-naphthylamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 119500, SDS-PAGE | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.83 | - |
Val-2-naphthylamide | - |
Homo sapiens | |
| 6.33 | - |
Ile-2-naphthylamide | - |
Homo sapiens | |
| 11.3 | - |
Lys-2-naphthylamide | - |
Homo sapiens | |
| 22.2 | - |
Arg-2-naphthylamide | - |
Homo sapiens | |
| 49.5 | - |
Leu-2-naphthylamide | - |
Homo sapiens | |
| 74.5 | - |
Phe-2-naphthylamide | - |
Homo sapiens | |
| 95.5 | - |
Met-2-naphthylamide | - |
Homo sapiens | |
| 141 | - |
Ala-2-naphthylamide | - |
Homo sapiens | |
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