BRENDA - Enzyme Database
show all sequences of 3.4.11.14

Human liver aminopeptidase. Role of metal ions in mechanism of action

Garner, C.W.; Behal, F.J.; Biochemistry 13, 3227-3233 (1974)

Data extracted from this reference:

General Stability
General Stability
Organism
when the enzyme is treated at 60°C with EDTA the inhibition is no longer reversible and there is a concomitant loss of zinc
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
99% inhibition at 0.5 mM; Ki 0.028 mM
Homo sapiens
2,2'-bipyridine
5.0 mM, 29% inhibition
Homo sapiens
2,9-Dimethyl-1,10-phenanthroline
2.0 mM, 52% inhibition
Homo sapiens
8-hydroxyquinoline
2.0 mM, 84% inhibition
Homo sapiens
azide
92% inhibition at 10 mM
Homo sapiens
cyanide
84% inhibition at 10 mM
Homo sapiens
EDTA
98% inhibition at 0.001 mM
Homo sapiens
EGTA
96% inhibition at 1 mM; Ki 0.012 mM
Homo sapiens
KCN
10 mM, 84% inhibition
Homo sapiens
Na2S
10 mM, 92% inhibition
Homo sapiens
oxalate
50 mM ammonium oxalate, 71% inhibition
Homo sapiens
Sulfide
-
Homo sapiens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
2.4fold activation, Co2+ binds loosely in a noncompetitive manner; Km: 0.05 mM
Homo sapiens
Zinc
8.3 nanoatoms of tightly bound zinc per mg of protein; zinc is located near the substrate binding site
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Homo sapiens
-
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.012
-
EGTA
-
Homo sapiens
0.028
-
1,10-phenanthroline
-
Homo sapiens
0.076
-
Sulfide
-
Homo sapiens
General Stability (protein specific)
General Stability
Organism
when the enzyme is treated at 60°C with EDTA the inhibition is no longer reversible and there is a concomitant loss of zinc
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
99% inhibition at 0.5 mM; Ki 0.028 mM
Homo sapiens
2,2'-bipyridine
5.0 mM, 29% inhibition
Homo sapiens
2,9-Dimethyl-1,10-phenanthroline
2.0 mM, 52% inhibition
Homo sapiens
8-hydroxyquinoline
2.0 mM, 84% inhibition
Homo sapiens
azide
92% inhibition at 10 mM
Homo sapiens
cyanide
84% inhibition at 10 mM
Homo sapiens
EDTA
98% inhibition at 0.001 mM
Homo sapiens
EGTA
96% inhibition at 1 mM; Ki 0.012 mM
Homo sapiens
KCN
10 mM, 84% inhibition
Homo sapiens
Na2S
10 mM, 92% inhibition
Homo sapiens
oxalate
50 mM ammonium oxalate, 71% inhibition
Homo sapiens
Sulfide
-
Homo sapiens
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.012
-
EGTA
-
Homo sapiens
0.028
-
1,10-phenanthroline
-
Homo sapiens
0.076
-
Sulfide
-
Homo sapiens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
2.4fold activation, Co2+ binds loosely in a noncompetitive manner; Km: 0.05 mM
Homo sapiens
Zinc
8.3 nanoatoms of tightly bound zinc per mg of protein; zinc is located near the substrate binding site
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Homo sapiens
-
Other publictions for EC 3.4.11.14
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
731336
Kruppa
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731338
Huber
Cyclin-dependent kinase 5 is a ...
Dictyostelium discoideum
Biochim. Biophys. Acta
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11-20
2013
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1
1
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731572
ODay
-
A tale of three proteins: The ...
Dictyostelium discoideum
Calcium Signal.
2012
119-130
2012
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717515
Chen
Identification of a puromycin- ...
Danio rerio
Comp. Biochem. Physiol. B
159
10-17
2011
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16
9
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1
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16
1
9
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1
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1
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9
1
1
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1
9
1
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1
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9
9
717651
Kudo
Puromycin-sensitive aminopepti ...
Homo sapiens
Hum. Mol. Genet.
20
1820-1833
2011
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1
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6
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1
1
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718137
Ren
Cu, Zn-superoxide dismutase 1 ...
Homo sapiens
Mol. Neurodegener.
6
29
2011
-
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1
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3
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1
1
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717649
Menzies
Puromycin-sensitive aminopepti ...
Homo sapiens
Hum. Mol. Genet.
19
4573-4586
2010
-
-
1
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-
-
4
-
1
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3
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1
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4
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1
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4
4
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1
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1
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2
2
-
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-
709501
Lee
Cobalt chloride-induced downre ...
Homo sapiens
J. Microbiol. Biotechnol.
19
530-536
2009
-
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1
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1
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1
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1
1
1
1
-
-
710523
Yanagi
Involvement of puromycin-sensi ...
Homo sapiens
Psychogeriatrics
9
157-166
2009
-
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1
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2
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1
1
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677547
Ewing
Detecting D-amino acid-contain ...
Sus scrofa
Anal. Chem.
80
2874-2880
2008
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1
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1
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681214
Towne
Puromycin-sensitive aminopepti ...
Mus musculus
J. Immunol.
180
1704-1712
2008
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1
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4
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700450
Cicin-Sain
Central aminopeptidase and ser ...
Rattus norvegicus
Neuropeptides
42
435-440
2008
-
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1
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3
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6
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6
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1
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677492
Varona
Altered levels of acid, basic, ...
Homo sapiens
Am. J. Physiol. Renal Physiol.
292
F780-F788
2007
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1
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1
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2
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2
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2
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1
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678982
Zambotti-Villela
Aspartyl, arginyl and alanyl a ...
Rattus norvegicus
Brain Res.
1170
112-118
2007
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1
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679494
Bhutani
Puromycin-sensitive aminopepti ...
Homo sapiens
EMBO J.
26
1385-1396
2007
-
-
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2
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1
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1
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4
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4
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680238
Bank
Inhibition of alanyl-aminopept ...
Mus musculus
Int. J. Mol. Med.
20
483-492
2007
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1
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1
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682118
Hui
Brain-specific aminopeptidase: ...
Mus musculus, Rattus norvegicus
Neurochem. Res.
32
2062-2071
2007
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4
1
3
1
1
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2
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7
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2
1
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4
1
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7
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2
1
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1
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1
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678175
Sengupta
Degradation of tau protein by ...
Homo sapiens
Biochemistry
45
15111-15119
2006
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1
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8
6
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2
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3
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6
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679432
Lyczak
The puromycin-sensitive aminop ...
Caenorhabditis elegans
Development
133
4281-4292
2006
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2
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680075
Banegas
Plasma aminopeptidase activiti ...
Homo sapiens
Horm. Metab. Res.
38
758-760
2006
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681000
Segarra
Atrial angiotensinase activity ...
Rattus norvegicus
J. Cardiovasc. Pharmacol.
48
117-120
2006
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1
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681191
Wherry
Re-evaluating the generation o ...
Mus musculus
J. Immunol.
176
2249-2261
2006
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1
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682124
Karsten
A genomic screen for modifiers ...
Homo sapiens
Neuron
51
549-560
2006
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2
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4
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7
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7
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A puromycin-sensitive aminopep ...
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Neuron-specific aminopeptidase ...
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Periplasmic aminopeptidases in ...
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Starnes
Human-liver alanine aminopepti ...
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Kao
Human kidney alanine aminopept ...
Homo sapiens
Biochemistry
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Hydrophobic binding sites of h ...
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Little
Human liver aminopeptidase ...
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Garner
Effect of pH on substrate and ...
Homo sapiens
Biochemistry
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1975
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Garner
Human liver alanine aminopepti ...
Homo sapiens
Biochemistry
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3208-3212
1975
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Starnes
A human liver aminopeptidase. ...
Homo sapiens
Biochemistry
13
3221-3227
1974
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647035
Garner
Human liver aminopeptidase. Ro ...
Homo sapiens
Biochemistry
13
3227-3233
1974
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