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Literature summary for 3.4.11.10 extracted from
- Aeromonas aminopeptidase: purification and some general properties (1966), Arch. Biochem. Biophys., 117, 328-336.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cysteine | 60% loss of activity at 10 mM | Vibrio proteolyticus |  |
| EDTA | - |
Vibrio proteolyticus | |
| Na2S | 60% loss of activity at 10 mM | Vibrio proteolyticus | |
| Thioglycollate | 60% loss of activity at 10 mM | Vibrio proteolyticus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Vibrio proteolyticus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 65% of activity of zinc enzyme | Vibrio proteolyticus | |
| Mn2+ | 58% of activity of zinc enzyme | Vibrio proteolyticus | |
| Zn2+ | - |
Vibrio proteolyticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29500 | - |
sedimentation velocity | Vibrio proteolyticus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Peptides + H2O | Vibrio proteolyticus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vibrio proteolyticus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Vibrio proteolyticus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 21 | - |
- |
Vibrio proteolyticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ala-Gly + H2O | - |
Vibrio proteolyticus | Ala + Gly | - |
ir | |
| DL-Ala-Gly-Gly + H2O | - |
Vibrio proteolyticus | DL-Ala + Gly-Gly | - |
ir | |
| DL-Leu-Gly-DL-Phe + H2O | - |
Vibrio proteolyticus | DL-Leu + Gly-DL-Phe | - |
ir | |
| DL-Leu-Gly-Gly + H2O | - |
Vibrio proteolyticus | DL-Leu + Gly-Gly | - |
ir | |
| Leu-beta-naphthylamide + H2O | - |
Vibrio proteolyticus | Leu + beta-naphthylamine | - |
ir | |
| Leu-Gly + H2O | - |
Vibrio proteolyticus | Leu + Gly | - |
ir | |
| Leu-Tyr + H2O | - |
Vibrio proteolyticus | Leu + Tyr | - |
ir | |
| Lys-Gly + H2O | - |
Vibrio proteolyticus | Lys + Gly | - |
ir | |
| Peptides + H2O | - |
Vibrio proteolyticus | ? | - |
? | |
| Phe-beta-naphthylamide + H2O | 18% of rate of hydrolysis of Leu-beta-naphthylamide | Vibrio proteolyticus | Phe + beta-naphthylamine | - |
ir | |
| Phe-Gly + H2O | - |
Vibrio proteolyticus | Phe + Gly | - |
ir | |
| Thr-beta-naphthylamide + H2O | 3.3% of rate of hydrolysis of Leu-beta-naphthylamide | Vibrio proteolyticus | Thr + beta-naphthylamine | - |
ir | |
| Tyr-Gly + H2O | - |
Vibrio proteolyticus | Tyr + Gly | - |
ir | |
| Val-Gly + H2O | - |
Vibrio proteolyticus | Val + Gly | - |
ir | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
below, but tolerates exposure up to a few hours | Vibrio proteolyticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Vibrio proteolyticus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8.5 | above 40% maximal activity across range | Vibrio proteolyticus |
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