Cloned (Comment) | Organism |
---|---|
His-tagged protein expressed in Escherichia coli MM294 | Vibrio proteolyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02726 | - |
recombinant human methionine-interferon alpha-2b | pH 6.0, 37°C | Vibrio proteolyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 32000, SDS-PAGE | Vibrio proteolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio proteolyticus | Q01693 | - |
- |
Purification (Comment) | Organism |
---|---|
from inclusion bodies, immobilized metal ion affinity chromatography (Ni2+), purity of 95% | Vibrio proteolyticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16 | - |
substrate L-leucine-4-nitroanilidepH 8.0, 37°C | Vibrio proteolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-leucine-4-nitroanilide + H2O | - |
Vibrio proteolyticus | L-leucine + 4-nitroaniline | - |
? | |
recombinant human methionine-interferon alpha-2b + H2O | - |
Vibrio proteolyticus | L-methionine + recombinant human interferon alpha-2b | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, SDS-PAGE | Vibrio proteolyticus |
Synonyms | Comment | Organism |
---|---|---|
leucine aminopeptidase | - |
Vibrio proteolyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Vibrio proteolyticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000853 | - |
recombinant human methionine-interferon alpha-2b | pH 6.0, 37°C | Vibrio proteolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Vibrio proteolyticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | - |
Vibrio proteolyticus |