Literature summary for 3.4.11.10 extracted from

Hua, Y.W.; Chi, M.C.; Lo, H.F.; Kuo, L.Y.; Ku, K.L.; Lin, L.L.
Adsorption-elution purification of chimeric Bacillus stearothermophilus leucine aminopeptidase II with raw-starch-binding activity (2005), World J. Microbiol. Biotechnol., 21, 689-694.

No PubMed abstract available

Search for more literature for 3.4.11.10

Cloned(Commentary)

Cloned (Comment)	Organism
expression of the chimeric enzyme comprising the raw-starch-binding domain of Bacillus sp. strain TS-23 alpha-amylase anthe Bacillus stearothermophilus LAPII in Escherichia coli strain M15	Geobacillus stearothermophilus

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of a chimeric enzyme comprising the raw-starch-binding domain of Bacillus sp. strain TS-23 alpha-amylase and the Bacillus stearothermophilus LAPII, the chimeric mutant enzyme binds raw starch, overview	Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetics of raw starch-binding by the chimeric mutant enzyme	Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
61000	-	x * 61000, recombinant chimeric enzyme, SDS-PAGE	Geobacillus stearothermophilus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant chimeric enzyme 20.4fold from Escherichia coli strain M15 by an affinity adsorption chromatographic step on a raw starch resin, screening of different elution methods, overview	Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
466.7	-	purified recombinant enzyme	Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Leu-4-nitroanilide + H2O	-	Geobacillus stearothermophilus	L-Leu + 4-nitroaniline	-	?

Subunits

Subunits	Comment	Organism
?	x * 61000, recombinant chimeric enzyme, SDS-PAGE	Geobacillus stearothermophilus

Synonyms

Synonyms	Comment	Organism
LAP	-	Geobacillus stearothermophilus
leucine aminopeptidase II	-	Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	assay at	Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Geobacillus stearothermophilus

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Release 2023.1 (January 2023)