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Literature summary for 3.4.11.10 extracted from

  • Lin, L.L.; Hsu, W.H.; Wu, C.P.; Chi, M.C.; Chou, W.M.; Hu, H.Y.
    A thermostable leucine aminopeptidase from Bacillus kaustophilus CCRC 11223 (2004), Extremophiles, 8, 79-87.
    View publication on PubMed
Search for more literature for 3.4.11.10

Cloned(Commentary)

Cloned (Comment) Organism
gene lap from genomic library, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli Geobacillus kaustophilus

Organism

Organism UniProt Comment Textmining
Geobacillus kaustophilus
-
-
-
Geobacillus kaustophilus CCRC 11223
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Geobacillus kaustophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Cys-4-nitroanilide + H2O
-
 Geobacillus kaustophilus L-Cys + 4-nitroaniline
-
 ?
L-Cys-4-nitroanilide + H2O
-
 Geobacillus kaustophilus CCRC 11223 L-Cys + 4-nitroaniline
-
 ?
L-Leu-4-nitroanilide + H2O preferred substrate Geobacillus kaustophilus L-Leu + 4-nitroaniline
-
 ?
L-Leu-4-nitroanilide + H2O preferred substrate Geobacillus kaustophilus CCRC 11223 L-Leu + 4-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
bacterial leucine aminopeptidase
-
 Geobacillus kaustophilus
LAP
-
 Geobacillus kaustophilus
thermostable leucine aminopeptidase
-
 Geobacillus kaustophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Geobacillus kaustophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 32 min, 50% remaining activity Geobacillus kaustophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Geobacillus kaustophilus