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Literature summary for 3.3.2.9 extracted from
- Ovarian expressed microsomal epoxide hydrolase role in detoxification of 4-vinylcyclohexene diepoxide and regulation by phosphatidylinositol-3 kinase signaling (2012), Toxicol. Appl. Pharmacol., 258, 118-23 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Ephx1 or meh, quantitative RT-PCR expression analysis | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cyclohexene oxide | a post-natal day 4 Fischer 344 rat whole ovary culture system inhibition of mEH using cyclohexene oxide during VCD exposure results in a greater loss of primordial and small primary follicles relative to VCD-treated ovaries | Rattus norvegicus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Rattus norvegicus | - |
- |
| microsome | - |
Mus musculus | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-vinylcyclohexene diepoxide + H2O | Rattus norvegicus | - |
4-(1,2-dihydroxy)ethyl-1,2-dihydroxycyclohexane | - |
? | |
| 4-vinylcyclohexene diepoxide + H2O | Mus musculus | - |
4-(1,2-dihydroxy)ethyl-1,2-dihydroxycyclohexane | - |
? | |
| 4-vinylcyclohexene diepoxide + H2O | Rattus norvegicus Fischer 344 | - |
4-(1,2-dihydroxy)ethyl-1,2-dihydroxycyclohexane | - |
? | |
| 7,12-dimethylbenz[a]anthracene + 3 H2O | Mus musculus | - |
7,12-dimethylbenz[a]anthra-3,4-diol-1,2-epoxide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | Q9D379 | - |
- |
| Rattus norvegicus | P07687 | - |
- |
| Rattus norvegicus Fischer 344 | P07687 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | microsomal epoxide hydrolase (mEH) is expressed in multiple tissues, including the ovary | Rattus norvegicus | - |
| additional information | microsomal epoxide hydrolase (mEH) is expressed in multiple tissues, including the ovary | Mus musculus | - |
| ovary | - |
Mus musculus | - |
| ovary | post-natal day 4, both primordial and small primary follicles | Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-vinylcyclohexene diepoxide + H2O | - |
Rattus norvegicus | 4-(1,2-dihydroxy)ethyl-1,2-dihydroxycyclohexane | - |
? | |
| 4-vinylcyclohexene diepoxide + H2O | - |
Mus musculus | 4-(1,2-dihydroxy)ethyl-1,2-dihydroxycyclohexane | - |
? | |
| 4-vinylcyclohexene diepoxide + H2O | - |
Rattus norvegicus Fischer 344 | 4-(1,2-dihydroxy)ethyl-1,2-dihydroxycyclohexane | - |
? | |
| 7,12-dimethylbenz[a]anthracene + 3 H2O | - |
Mus musculus | 7,12-dimethylbenz[a]anthra-3,4-diol-1,2-epoxide | - |
? | |
| additional information | microsomal epoxide hydrolase (mEH) has a wide substrate specificity | Mus musculus | ? | - |
? | |
| additional information | microsomal epoxide hydrolase (mEH) has wide a substrate specificity | Rattus norvegicus | ? | - |
? | |
| additional information | microsomal epoxide hydrolase (mEH) has wide a substrate specificity | Rattus norvegicus Fischer 344 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EPHX1 | - |
Rattus norvegicus |
| mEH | - |
Rattus norvegicus |
| mEH | - |
Mus musculus |
| Microsomal epoxide hydrolase | - |
Rattus norvegicus |
| Microsomal epoxide hydrolase | - |
Mus musculus |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Mus musculus | 4-vinylcyclohexene diepoxide (VCD) increases mRNA and protein expression of microsomal epoxide hydrolase (mEH). mEH mRNA and enzyme activity expression are shown to increase in small pre-antral follicles of mouse ovaries following repeated daily dosing (15d) with VCD | up |
| Rattus norvegicus | relative to controls, meh mRNA is increased on day 4 of 4-vinylcyclohexene diepoxide exposure, followed by increased mEH protein after 6 days. Inhibition of phosphatidyinositol-3 kinase signaling increases mEH mRNA and protein expression | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the microsomal epoxide hydrolase plays a role in detoxification of 4-vinylcyclohexene diepoxide and is regulated by phosphatidylinositol-3 kinase signaling (PI3K). 4-Vinylcyclohexene diepoxide (VCD) is a metabolite of 4-vinylcyclohexene (VCH) which has the potential to be formed in the ovary through CYP2E1 activity. Functional role for mEH in the rat ovary involvement of PI3K signaling in regulation of ovarian xenobiotic metabolism by mEH | Rattus norvegicus |
| physiological function | the microsomal epoxide hydrolase plays a role in detoxification of 4-vinylcyclohexene diepoxide and is regulated by phosphatidylinositol-3 kinase signaling. 4-Vinylcyclohexene diepoxide (VCD) is a metabolite of 4-vinylcyclohexene (VCH) which has the potential to be formed in the ovary through CYP2E1 activity. The inactive tetrol metabolite (4-(1,2-dihydroxy)ethyl-1,2-dihydroxycyclohexane) can be formed in mouse ovarian follicles, potentially through detoxification action of mEH. mEH can bioactivate the ovotoxic chemical 7,12-dimethylbenz[a]anthracene (DMBA) to the more toxic compound, DMBA-3,4-diol-1,2-epoxide | Mus musculus |
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