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Literature summary for 3.3.2.9 extracted from

  • Kamita, S.G.; Yamamoto, K.; Dadala, M.M.; Pha, K.; Morisseau, C.; Escaich, A.; Hammock, B.D.
    Cloning and characterization of a microsomal epoxide hydrolase from Heliothis virescens (2013), Insect Biochem. Mol. Biol., 43, 219-228.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.3.2.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf9 insect cells Heliothis virescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0032
-
 juvenile hormone III in 100 mM sodium phosphate, pH 8.0, at 30°C Heliothis virescens

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Heliothis virescens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53129
-
 x * 53129, calculated from amino acid sequence Heliothis virescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cis-stilbene oxide + H2O Heliothis virescens
-
 (+)-(1R,2R)-1,2-diphenylethane-1,2-diol
-
 ?
juvenile hormone III + H2O Heliothis virescens poor substrate ?
-
 ?
trans-diphenylpropene oxide + H2O Heliothis virescens
-
 ?
-
 ?
trans-stilbene oxide + H2O Heliothis virescens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Heliothis virescens L7R9X8
-
-

Source Tissue

Source Tissue Comment Organism Textmining
fat body
-
 Heliothis virescens
-
larva
-
 Heliothis virescens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00003
-
 with cyano(6-methoxy-naphthalen-2-yl)methyl oxiran-2-ylmethyl carbonate as substrate, in 100 mM sodium phosphate, pH 8.0, at 30°C Heliothis virescens
0.0001
-
 with trans-diphenylpropene oxide as substrate, in 100 mM sodium phosphate, pH 8.0, at 30°C Heliothis virescens
0.0003
-
 with trans-stilbene oxide as substrate, in 100 mM sodium phosphate, pH 8.0, at 30°C Heliothis virescens
0.0017
-
 with juvenile hormone III as substrate, in 100 mM sodium phosphate, pH 8.0, at 30°C Heliothis virescens
0.0037
-
 with cyano-(6-methoxy-naphthalen-2-yl)-methyl 3,3-dimethyl-oxiranylmethyl carbonate as substrate, in 100 mM sodium phosphate, pH 8.0, at 30°C Heliothis virescens
0.0458
-
 with cis-stilbene oxide as substrate, in 100 mM sodium phosphate, pH 8.0, at 30°C Heliothis virescens
0.122
-
 with cyano-(6-methoxy-naphthalen-2-yl)-methyl trans-((3-ethyl-oxiran-2-yl)methyl) carbonate as substrate, in 100 mM sodium phosphate, pH 8.0, at 30°C Heliothis virescens
0.138
-
 with cyano(2-methoxynaphthalen-6-yl)methyl trans-(2-(3-phenyloxiran-2-yl))acetate as substrate, in 100 mM sodium phosphate, pH 8.0, at 30°C Heliothis virescens
0.816
-
 with cyano(6-methoxy-naphthalen-2-yl)methyl trans-[(3-phenyloxiran-2-yl)methyl]carbonate as substrate, in 100 mM sodium phosphate, pH 8.0, at 30°C Heliothis virescens
1.18
-
 with cyano(2-methoxynaphthalen-6-yl)methyl trans-2-(3-propyloxiran-2-yl)acetate as substrate, in 100 mM sodium phosphate, pH 8.0, at 30°C Heliothis virescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-stilbene oxide + H2O
-
 Heliothis virescens (+)-(1R,2R)-1,2-diphenylethane-1,2-diol
-
 ?
cyano(2-methoxynaphthalen-6-yl)methyl trans-(2-(3-phenyloxiran-2-yl))acetate + H2O
-
 Heliothis virescens ?
-
 ?
cyano(2-methoxynaphthalen-6-yl)methyl trans-2-(3-propyloxiran-2-yl)acetate + H2O
-
 Heliothis virescens ?
-
 ?
cyano(6-methoxy-naphthalen-2-yl)methyl oxiran-2-ylmethyl carbonate + H2O
-
 Heliothis virescens ?
-
 ?
cyano(6-methoxy-naphthalen-2-yl)methyl trans-[(3-phenyloxiran-2-yl)methyl]carbonate + H2O
-
 Heliothis virescens ?
-
 ?
cyano-(6-methoxy-naphthalen-2-yl)-methyl 3,3-dimethyl-oxiranylmethyl carbonate + H2O
-
 Heliothis virescens ?
-
 ?
cyano-(6-methoxy-naphthalen-2-yl)-methyl trans-((3-ethyl-oxiran-2-yl)methyl) carbonate + H2O
-
 Heliothis virescens ?
-
 ?
juvenile hormone III + H2O poor substrate Heliothis virescens ?
-
 ?
trans-diphenylpropene oxide + H2O
-
 Heliothis virescens ?
-
 ?
trans-stilbene oxide + H2O
-
 Heliothis virescens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 53129, calculated from amino acid sequence Heliothis virescens

Synonyms

Synonyms Comment Organism
mEH
-
 Heliothis virescens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.002
-
 juvenile hormone III in 100 mM sodium phosphate, pH 8.0, at 30°C Heliothis virescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 with juvenile hormone II as substrate Heliothis virescens

pI Value

Organism Comment pI Value Maximum pI Value
Heliothis virescens calculated from amino acid sequence
-
 7.2

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
630
-
 juvenile hormone III in 100 mM sodium phosphate, pH 8.0, at 30°C Heliothis virescens