Application | Comment | Organism |
---|---|---|
analysis | development of a fluorescence-based assay using substrate cyano(6-methoxy-naphthalen-2-yl)methyl glycidyl carbonate and application as a useful tool for the discovery of structure-activity relationships among mEH inhibitors and for the screening chemical library with high accuracy and with a Z' value of approximately 0.7 | Homo sapiens |
analysis | development of a fluorescence-based assay using substrate cyano(6-methoxy-naphthalen-2-yl)methyl glycidyl carbonate and application as a useful tool for the discovery of structure-activity relationships among mEH inhibitors and for the screening chemical library with high accuracy and with a Z' value of approximately 0.7 | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
- |
Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(9E)-octadec-9-enamide | - |
Homo sapiens | |
(9E)-octadec-9-enamide | - |
Rattus norvegicus | |
12-hydroxyoctadecanamide | - |
Homo sapiens | |
12-hydroxyoctadecanamide | - |
Rattus norvegicus | |
2-(nonylsulfanyl)propanamide | - |
Homo sapiens | |
2-(nonylsulfanyl)propanamide | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0023 | - |
cyano(6-methoxy-naphthalen-2-yl)methyl glycidyl carbonate | pH 9.0, 37°C | Rattus norvegicus | |
0.0038 | - |
cyano(6-methoxy-naphthalen-2-yl)methyl glycidyl carbonate | pH 9.0, 37°C | Homo sapiens | |
0.012 | - |
4-methyl-2-oxo-2H-chromen-7-yl oxiran-2-ylmethyl carbonate | pH 9.0, 37°C | Homo sapiens | |
0.022 | - |
4-methyl-2-oxo-2H-chromen-7-yl oxiran-2-ylmethyl carbonate | pH 9.0, 37°C | Rattus norvegicus | |
0.035 | - |
cis-stilbene oxide | pH 9.0, 37°C | Rattus norvegicus | |
0.141 | - |
cis-stilbene oxide | pH 9.0, 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.021 | - |
substrate 4-methyl-2-oxo-2H-chromen-7-yl oxiran-2-ylmethyl carbonate, pH 9.0, 37°C | Homo sapiens |
0.023 | - |
substrate 4-nitrophenyl 3-(oxiran-2-yl)propanoate, pH 9.0, 37°C | Rattus norvegicus |
0.025 | - |
substrate cyano(6-methoxy-naphthalen-2-yl)methyl glycidyl carbonate, pH 9.0, 37°C | Homo sapiens |
0.029 | - |
substrate 4-nitrophenyl 6-oxabicyclo[3.1.0]hexane-3-carboxylate, pH 9.0, 37°C | Rattus norvegicus |
0.048 | - |
substrate 4-methyl-2-oxo-2H-chromen-7-yl oxiran-2-ylmethyl carbonate, pH 9.0, 37°C | Rattus norvegicus |
0.051 | - |
substrate 4-nitrophenyl 6-oxabicyclo[3.1.0]hexane-3-carboxylate, pH 9.0, 37°C | Homo sapiens |
0.068 | - |
substrate cyano(6-methoxy-naphthalen-2-yl)methyl glycidyl carbonate, pH 9.0, 37°C | Rattus norvegicus |
0.072 | - |
substrate 4-nitrophenyl 3-(oxiran-2-yl)propanoate, pH 9.0, 37°C | Homo sapiens |
Storage Stability | Organism |
---|---|
4°C, stable for at least 3 months | Homo sapiens |
4°C, stable for at least 3 months | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methyl-2-oxo-2H-chromen-7-yl oxiran-2-ylmethyl carbonate + H2O | - |
Homo sapiens | ? | - |
? | |
4-methyl-2-oxo-2H-chromen-7-yl oxiran-2-ylmethyl carbonate + H2O | - |
Rattus norvegicus | ? | - |
? | |
4-nitrophenyl 3-(oxiran-2-yl)propanoate + H2O | - |
Homo sapiens | ? | - |
? | |
4-nitrophenyl 3-(oxiran-2-yl)propanoate + H2O | - |
Rattus norvegicus | ? | - |
? | |
4-nitrophenyl 6-oxabicyclo[3.1.0]hexane-3-carboxylate + H2O | - |
Homo sapiens | ? | - |
? | |
4-nitrophenyl 6-oxabicyclo[3.1.0]hexane-3-carboxylate + H2O | - |
Rattus norvegicus | ? | - |
? | |
cis-stilbene oxide + H2O | - |
Homo sapiens | (1R,2R)-1,2-diphenylethane-1,2-diol | - |
? | |
cis-stilbene oxide + H2O | - |
Rattus norvegicus | (1R,2R)-1,2-diphenylethane-1,2-diol | - |
? | |
cyano(6-methoxy-naphthalen-2-yl)methyl glycidyl carbonate + H2O | synthetic fluorescent substrate | Homo sapiens | ? | - |
? | |
cyano(6-methoxy-naphthalen-2-yl)methyl glycidyl carbonate + H2O | synthetic fluorescent substrate | Rattus norvegicus | ? | - |
? |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0004 | - |
substrate cis-stilbene oxide, pH 9.0, 37°C | Rattus norvegicus | 2-(nonylsulfanyl)propanamide | |
0.0005 | - |
substrate cis-stilbene oxide, pH 9.0, 37°C | Homo sapiens | 2-(nonylsulfanyl)propanamide | |
0.0005 | - |
substrate cis-stilbene oxide, pH 9.0, 37°C | Rattus norvegicus | (9E)-octadec-9-enamide | |
0.001 | - |
substrate cis-stilbene oxide, pH 9.0, 37°C | Homo sapiens | (9E)-octadec-9-enamide | |
0.0011 | - |
substrate cis-stilbene oxide, pH 9.0, 37°C | Rattus norvegicus | 12-hydroxyoctadecanamide | |
0.0026 | - |
substrate cis-stilbene oxide, pH 9.0, 37°C | Homo sapiens | 12-hydroxyoctadecanamide | |
0.0042 | - |
substrate cyano(6-methoxy-naphthalen-2-yl)methyl glycidyl carbonate, pH 9.0, 37°C | Rattus norvegicus | 2-(nonylsulfanyl)propanamide | |
0.006 | - |
substrate cyano(6-methoxy-naphthalen-2-yl)methyl glycidyl carbonate, pH 9.0, 37°C | Homo sapiens | 2-(nonylsulfanyl)propanamide | |
0.063 | - |
substrate cyano(6-methoxy-naphthalen-2-yl)methyl glycidyl carbonate, pH 9.0, 37°C | Rattus norvegicus | (9E)-octadec-9-enamide | |
0.123 | - |
substrate cyano(6-methoxy-naphthalen-2-yl)methyl glycidyl carbonate, pH 9.0, 37°C | Rattus norvegicus | 12-hydroxyoctadecanamide | |
0.14 | - |
substrate cyano(6-methoxy-naphthalen-2-yl)methyl glycidyl carbonate, pH 9.0, 37°C | Homo sapiens | (9E)-octadec-9-enamide | |
0.2 | - |
or above, substrate cyano(6-methoxy-naphthalen-2-yl)methyl glycidyl carbonate, pH 9.0, 37°C | Homo sapiens | 12-hydroxyoctadecanamide |