Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | insulin increases enzyme activity in hepatocyte cell culture | Rattus norvegicus | |
Phospholipids | the enzyme is tightly associated with phospholipids | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
gene EPHX1 is located on chromosome 1, DNA and amino acid sequence determination and analysis, expression of liver enzyme in Schizosaccharomyces pombe, expression in Cos-1 cells, expression using the baculovirus system | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E404D | mutation of the catalytic triad residue leads to increased activity compared to the wild-type enzyme | Homo sapiens |
additional information | polymorphisms associated with the onset of diseases | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | suppression of enzyme expression by glucocorticoid, interacting with the 5'-flanking sequence, and by dexamethasone, gadolinium chloride, acriflavine, lipopolysaccharide, gem-di-, trans-di-, tri- and tetra-substituted epoxides are either low turnover substrates or inhibitors | Homo sapiens | |
additional information | suppression of enzyme expression by dexamethasone, gadolinium chloride, acriflavine, lipopolysaccharide | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | in neoplastic livers | Homo sapiens | 5829 | - |
microsome | - |
Mus musculus | - |
- |
microsome | - |
Homo sapiens | - |
- |
microsome | - |
Rattus norvegicus | - |
- |
additional information | the enzyme shows strong hydrophobic interaction with the membrane, deletion of the N-terminal membrane anchor does not render the enzyme soluble | Homo sapiens | - |
- |
plasma membrane | in liver, the catalytic site facing the extracellular medium | Homo sapiens | 5886 | - |
smooth endoplasmic reticulum | in liver, the catalytic site facing the cytosol | Homo sapiens | 5790 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, about | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | key enzyme in the metabolism of environmental contaminants being responsible for xenobiotic transformations, regulation of the enzyme occurs at transcriptional, translational, and post-translational level, the enzyme is also involved in cytoprotection and steroid metabolism, as well as in cellular responses to glucose metabolism and in Na+-dependent bile acid transport, the enzyme is part of a multi-transport system at the cell surface | ? | - |
? | |
additional information | Mus musculus | key enzyme in the metabolism of environmental contaminants being responsible for xenobiotic transformations, regulation of the enzyme occurs at transcriptional, translational, and post-translational level. The enzyme is also involved in cytoprotection and steroid metabolism, as well as in cellular responses to glucose metabolism and in Na+-dependent bile acid transport. The enzyme is part of a multi-transport system at the cell surface | ? | - |
? | |
additional information | Rattus norvegicus | the enzyme activity in diabetic and in fasted rats is reduced by 60-71%, key enzyme in the metabolism of environmental contaminants being responsible for xenobiotic transformations, regulation of the enzyme occurs at transcriptional, translational, and post-translational level, the enzyme is also involved in cytoprotection and steroid metabolism, as well as in cellular responses to glucose metabolism and in Na+-dependent bile acid transport, the enzyme is part of a multi-transport system at the cell surface | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | - |
Homo sapiens | - |
adrenal gland | adrenalectomy elevates enzyme levels | Rattus norvegicus | - |
brain | - |
Mus musculus | - |
brain | - |
Homo sapiens | - |
brain | - |
Rattus norvegicus | - |
bronchus | - |
Homo sapiens | - |
cell culture | numerous cancer cell lines and primary cells | Homo sapiens | - |
epithelium | bronchial | Homo sapiens | - |
glial cell | high enzyme content | Mus musculus | - |
glial cell | high enzyme content | Homo sapiens | - |
glial cell | high enzyme content | Rattus norvegicus | - |
heart | - |
Mus musculus | - |
heart | - |
Rattus norvegicus | - |
hepatocyte | - |
Rattus norvegicus | - |
hypothalamus | - |
Rattus norvegicus | - |
liver | - |
Mus musculus | - |
liver | - |
Homo sapiens | - |
liver | the liver enzyme is controlled by the pituitary gland | Rattus norvegicus | - |
lung | - |
Mus musculus | - |
lung | - |
Homo sapiens | - |
lung | - |
Rattus norvegicus | - |
lymphocyte | - |
Homo sapiens | - |
lymphocyte | intra-epithelial, cells show down-regulated enzyme expression | Mus musculus | - |
monocyte | - |
Homo sapiens | - |
additional information | wide tissue distribution in mammalian tissue, enzyme levels vary with environmental exposure, sex, and age | Mus musculus | - |
additional information | wide tissue distribution in mammalian tissue, enzyme levels vary with environmental exposure, sex, and age | Homo sapiens | - |
additional information | wide tissue distribution in mammalian tissue, enzyme levels vary with environmental exposure, sex, and age, growth hormones are involved in sexually dimorphic liver enzyme expression | Rattus norvegicus | - |
neuron | low enzyme content | Mus musculus | - |
neuron | low enzyme content | Homo sapiens | - |
neuron | low enzyme content | Rattus norvegicus | - |
ovary | - |
Mus musculus | - |
pituitary gland | hypophysectomy induces the liver enzyme in females and males | Rattus norvegicus | - |
synovial tissue | - |
Homo sapiens | - |
testis | - |
Mus musculus | - |
testis | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
epoxy-stearic acid + H2O | highly enantiospecific reaction, low activity | Homo sapiens | ? | - |
? | |
additional information | key enzyme in the metabolism of environmental contaminants being responsible for xenobiotic transformations, regulation of the enzyme occurs at transcriptional, translational, and post-translational level, the enzyme is also involved in cytoprotection and steroid metabolism, as well as in cellular responses to glucose metabolism and in Na+-dependent bile acid transport, the enzyme is part of a multi-transport system at the cell surface | Homo sapiens | ? | - |
? | |
additional information | key enzyme in the metabolism of environmental contaminants being responsible for xenobiotic transformations, regulation of the enzyme occurs at transcriptional, translational, and post-translational level. The enzyme is also involved in cytoprotection and steroid metabolism, as well as in cellular responses to glucose metabolism and in Na+-dependent bile acid transport. The enzyme is part of a multi-transport system at the cell surface | Mus musculus | ? | - |
? | |
additional information | the enzyme activity in diabetic and in fasted rats is reduced by 60-71%, key enzyme in the metabolism of environmental contaminants being responsible for xenobiotic transformations, regulation of the enzyme occurs at transcriptional, translational, and post-translational level, the enzyme is also involved in cytoprotection and steroid metabolism, as well as in cellular responses to glucose metabolism and in Na+-dependent bile acid transport, the enzyme is part of a multi-transport system at the cell surface | Rattus norvegicus | ? | - |
? | |
additional information | wide substrate specificity, epoxide-containing glycerol-phospholipids are poor substrates, the enzyme prefers mono- and cis-1,2-disubstituted epoxides as substrates, while gem-di-, trans-di-, tri- and tetra-substituted epoxides are either low turnover substrates or inhibitors, the enzyme has a tamaoxifen binding site | Homo sapiens | ? | - |
? | |
additional information | wide substrate specificity, the enzyme prefers mono- and cis-1,2-disubstituted epoxides as substrates, while gem-di-, trans-di-, tri- and tetra-substituted epoxides are either low turnover substrates or inhibitors | Mus musculus | ? | - |
? | |
additional information | wide substrate specificity, the enzyme prefers mono- and cis-1,2-disubstituted epoxides as substrates, while gem-di-, trans-di-, tri- and tetra-substituted epoxides are either low turnover substrates or inhibitors | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, about | Homo sapiens |
More | the enzyme possesses an N-terminal strong hydrophobic transmembrane anchor | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
EPHX1 | - |
Homo sapiens |
mEH | - |
Mus musculus |
mEH | - |
Homo sapiens |
mEH | - |
Rattus norvegicus |