Literature summary for 3.3.2.9 extracted from

Seidegard, J.; DePierre, J.W.
Microsomal epoxide hydrolase. Properties, regulation and function (1983), Biochim. Biophys. Acta, 695, 251-270.

Activating Compound

Activating Compound	Comment	Organism
1(2-naphthyl)-2-phenylethanedione	stimulates	Rattus norvegicus
benzil	stimulates	Rattus norvegicus
chalcone epoxide	stimulates	Rattus norvegicus
clotrimazole	stimulates	Rattus norvegicus
ellipticine	stimulates	Rattus norvegicus
flavone	stimulates	Rattus norvegicus
Harmane	stimulates	Rattus norvegicus
Isoquinoline	stimulates	Rattus norvegicus
Metyrapone	-	Oryctolagus cuniculus
Metyrapone	stimulates	Rattus norvegicus
nitroanisil	stimulates	Rattus norvegicus
norharmane	stimulates	Rattus norvegicus
p-anisil	stimulates	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
microsome	-	Rattus norvegicus	-	-
microsome	-	Oryctolagus cuniculus	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Rattus norvegicus	enzyme is involved in the metabolism of steroids, the microsomal enzyme plays a central role in both the inactivation of primary mutagenic and carcinogenic metabolites of polycyclic aromatic hydrocarbons, and in activating these metabolites to even more toxic or mutagenic secondary products, enzyme production is induced by 3-methylcholanthrene, phenobarbital, trans-stilbene oxide, 2(3)-tert-butyl-4-hydroxyanisol or 2-acetylaminofluorene	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Oryctolagus cuniculus	-	-	-
Rattus norvegicus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Rattus norvegicus	-
liver	-	Oryctolagus cuniculus	-

Storage Stability

Storage Stability	Organism
0°C, 50 mM sodium phosphate buffer, pH 7.0, 2 months, stable	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
1,2,3,4,9,9-hexachloro-6,7-epoxy-1,4,41,5,6,7,8,8a-octahydro-1,4-methanonaphthalene + H2O	-	Rattus norvegicus	?	-	?
1,2,3,4,9,9-hexachloro-6,7-epoxy-1,4,41,5,6,7,8,8a-octahydro-1,4-methanonaphthalene + H2O	-	Oryctolagus cuniculus	?	-	?
androstene oxide + H2O	-	Rattus norvegicus	?	-	?
benzene oxide + H2O	-	Rattus norvegicus	?	-	?
benzoanthracene 5,6-oxide + H2O	-	Rattus norvegicus	?	-	?
benzopyrene 11,12-oxide + H2O	-	Rattus norvegicus	?	-	?
benzopyrene 4,5-oxide + H2O	-	Rattus norvegicus	(-)benzopyrene 4,5-dihydrodiol	-	?
benzopyrene 4,5-oxide + H2O	-	Oryctolagus cuniculus	(-)benzopyrene 4,5-dihydrodiol	-	?
benzopyrene 7,8-oxide + H2O	-	Rattus norvegicus	?	-	?
benzopyrene 9,10-oxide + H2O	-	Rattus norvegicus	?	-	?
cis-stilbene oxide + H2O	-	Rattus norvegicus	?	-	?
cis-stilbene oxide + H2O	-	Oryctolagus cuniculus	?	-	?
dibenzoanthracene + H2O	-	Rattus norvegicus	?	-	?
estroxide + H2O	-	Rattus norvegicus	?	-	?
additional information	enzyme is involved in the metabolism of steroids, the microsomal enzyme plays a central role in both the inactivation of primary mutagenic and carcinogenic metabolites of polycyclic aromatic hydrocarbons, and in activating these metabolites to even more toxic or mutagenic secondary products, enzyme production is induced by 3-methylcholanthrene, phenobarbital, trans-stilbene oxide, 2(3)-tert-butyl-4-hydroxyanisol or 2-acetylaminofluorene	Rattus norvegicus	?	-	?
naphthalene-1,2-oxide + H2O	-	Rattus norvegicus	?	-	?
octene 1,2-oxide + H2O	-	Rattus norvegicus	?	-	?
phenanthrene-9,10-oxide + H2O	-	Rattus norvegicus	?	-	?
styrene 7,8-oxide + H2O	-	Rattus norvegicus	styrene glycol	-	?
trans-ethyl styrene oxide + H2O	-	Rattus norvegicus	?	-	?
trans-ethyl styrene oxide + H2O	-	Oryctolagus cuniculus	?	-	?

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.9	9.4	hydration of styrene 7,8-oxide	Rattus norvegicus