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Literature summary for 3.3.2.8 extracted from

  • Arand, M.; Hallberg, B.M.; Zou, J.; Bergfors, T.; Oesch, F.; Van Der Werf, M.J.; De Bont, J.A.M.; Jones, T.A.; Mowbray, S.L.
    Structure of Rhodococcus erythropolis limonene-1,2-epoxide hydrolase reveals a novel active site (2003), EMBO J., 22, 2583-2592.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.3.2.8

Crystallization (Commentary)

Crystallization (Comment) Organism
selenomethionine-substituted enzyme Rhodococcus erythropolis

Protein Variants

Protein Variants Comment Organism
D101A catalytically inactive, D101 is the acid catalyst that protonates the epoxide oxygen Rhodococcus erythropolis
D101N catalytically inactive, D101 is the acid catalyst that protonates the epoxide oxygen Rhodococcus erythropolis
N55A little residual activity Rhodococcus erythropolis
N55D catalytically inactive Rhodococcus erythropolis
N55D/D132N catalytically inactive, folding correct Rhodococcus erythropolis
R99A catalytically inactive Rhodococcus erythropolis
R99H catalytically inactive Rhodococcus erythropolis
R99K catalytically inactive Rhodococcus erythropolis
R99Q catalytically inactive Rhodococcus erythropolis
Y53F partially active Rhodococcus erythropolis

Inhibitors

Inhibitors Comment Organism Structure
hexanamide competitive Rhodococcus erythropolis
Hexylamine competitive Rhodococcus erythropolis
valpromide i.e. dipropylacetamide, competitive Rhodococcus erythropolis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4
-
 styrene-7,8-oxide pH 7.4, mutant N55A Rhodococcus erythropolis
1.4
-
 styrene-7,8-oxide pH 7.4, wild-type Rhodococcus erythropolis
3.7
-
 styrene-7,8-oxide pH 7.4, mutant Y53F Rhodococcus erythropolis

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis Q9ZAG3
-
-

Reaction

Reaction Comment Organism Reaction ID
1,2-epoxymenth-8-ene + H2O = menth-8-ene-1,2-diol one-step mechanism Rhodococcus erythropolis
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
styrene-7,8-oxide + H2O
-
 Rhodococcus erythropolis styrene-7,8-diol
-
 ?

Subunits

Subunits Comment Organism
dimer crystallization data Rhodococcus erythropolis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.002
-
 styrene-7,8-oxide pH 7.4, mutant N55A Rhodococcus erythropolis
0.176
-
 styrene-7,8-oxide pH 7.4, mutant Y53F Rhodococcus erythropolis
0.47
-
 styrene-7,8-oxide pH 7.4, wild-type Rhodococcus erythropolis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.035
-
 Hexylamine pH 7.4, 1% ethanol in final assay mixture Rhodococcus erythropolis
0.1
-
 valpromide pH 7.4, 1% ethanol in final assay mixture Rhodococcus erythropolis
2
-
 hexanamide pH 7.4, 1% ethanol in final assay mixture Rhodococcus erythropolis