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Literature summary for 3.3.2.8 extracted from
- Limonene-1,2-epoxide hydrolase from Rhodococcus erythropolis DCL14 belongs to a novel class of epoxide hydrolases (1998), J. Bacteriol., 180, 5052-5057.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 15000 | - |
gel filtration | Rhodococcus erythropolis |
| 17000 | - |
SDS-PAGE | Rhodococcus erythropolis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus erythropolis | - |
enzyme is induced if cells are grown on monoterpenes | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gel filtration, hydroxyapatite, anion exchange | Rhodococcus erythropolis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 85.1 | - |
- |
Rhodococcus erythropolis |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | gel filtration | Rhodococcus erythropolis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Rhodococcus erythropolis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 45 | rapid inactivation above 45°C | Rhodococcus erythropolis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
very broad pH optimum | Rhodococcus erythropolis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10.5 | below pH 5 chemical hydrolysis overtook biological hydrolysis rate | Rhodococcus erythropolis |
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Release 2023.1 (January 2023)
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