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Literature summary for 3.3.2.6 extracted from

  • Bito, H.; Ohishi, N.; Miki, I.; Minami, M.; Tanabe, T.; Shimizu, T.; Seyama, Y.
    Leukotriene A4 hydrolase from guinea pig lung: the presence of two catalytically active forms (1989), J. Biochem., 105, 261-264.
    View publication on PubMed
Search for more literature for 3.3.2.6

Application

Application Comment Organism
medicine the enzyme catalyses the hydrolysis of leukotriene A4 into the proinflammatory substance leukotriene B4 Cavia porcellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 leukotriene A4 pH 7.8, 37°C Cavia porcellus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 gel filtration Cavia porcellus
70000
-
 x * 70000, SDS-PAGE Cavia porcellus

Organism

Organism UniProt Comment Textmining
Cavia porcellus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cavia porcellus

Source Tissue

Source Tissue Comment Organism Textmining
lung
-
 Cavia porcellus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.53
-
-
 Cavia porcellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
leukotriene A4 + H2O
-
 Cavia porcellus leukotriene B4
-
 ?

Subunits

Subunits Comment Organism
? x * 70000, SDS-PAGE Cavia porcellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6 8.9
-
 Cavia porcellus

pI Value

Organism Comment pI Value Maximum pI Value
Cavia porcellus chromatofocusing, isoelectric focusing, in absence of SH reducing reagents
-
 5.4
Cavia porcellus chromatofocusing, isoelectric focusing, in presence of SH reducing reagents
-
 5.7