Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dinitroanisole + H2O | Nocardioides sp. JS1661 | - |
methanol + 2,4-dinitrophenol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nocardioides sp. JS1661 | A0A096ZEC9 AND A0A096ZED0 | subunit alpha and subunit beta, encoded by genes dnhA and dnhB | - |
Purification (Comment) | Organism |
---|---|
native enzyme from strain JS1661 by ammonium sulfate fractionation, hydrophobic interaction chromatography, and ultrafiltration | Nocardioides sp. JS1661 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dinitroanisole + H2O | - |
Nocardioides sp. JS1661 | methanol + 2,4-dinitrophenol | - |
? | |
2,4-dinitroanisole + H2O | stoichiometric formation of 2,4-dinitrophenol | Nocardioides sp. JS1661 | methanol + 2,4-dinitrophenol | - |
? | |
additional information | alkaline hydrolysis of DNAN is associated with considerable C and N isotope fractionation, isotopic analyses by gas chromatography and isotope ratio mass spectrometry (GC/IRMS). Carbon and nitrogen isotope enrichment factors (epsilonC and epsilonN), apparent 13C and 15N kinetic isotope effects, and correlations of C and N isotope fractionation (DELTAN/C) associated with the alkaline and enzymatic hydrolysis of DNAN, overview | Nocardioides sp. JS1661 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNAN O-demethylase | - |
Nocardioides sp. JS1661 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Nocardioides sp. JS1661 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at, intact cells | Nocardioides sp. JS1661 |
7.5 | - |
assay at, partially purified enzyme | Nocardioides sp. JS1661 |
General Information | Comment | Organism |
---|---|---|
additional information | evaluation of the C and N isotope fractionation associated with abiotic and biological 2,4-dinitroanisole (DNAN) hydrolysis through alkaline hydrolysis at high pH as well as enzymatic hydrolysis by Nocardioides sp. JS1661 and partially purified DNAN O-demethylase for decontamination of DNAN. Whereas both reactions generate 2,4-dinitrophenol (DNP), compound-specific isotope analysis (CSIA) of DNAN and DNP reveal that these reactions occur by different mechanisms, thus different mechanisms of alkaline and enzymatic hydrolysis of the insensitive munition component 2,4-dinitroanisole lead to identical products | Nocardioides sp. JS1661 |